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Article
Disrupting Na+,HCO3–-cotransporter NBCn1 (Slc4a7) delays murine breast cancer development
Increased metabolism and insufficient blood supply cause acidic waste product accumulation in solid cancers. During carcinogenesis, cellular acid extrusion is upregulated but the underlying molecular mechanism...
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Chapter
Tissue Processing: III. Fixation, General Aspects
Fixation is used in this text to denote a chemical treatment leading to immobilization and stabilization of tissue components. Displacement and extraction of native components should be minimal and, ideally, thos...
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Chapter
Enzyme Histochemistry I: General Considerations
Enzymes are proteins or glycoproteins with selective, often specific, catalytic effects. They characteristically increase the rates of reactions by a factor of at least 107.
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Chapter
General Theory for Tissue Staining
Although the specific characteristics of the tissue and the choice of staining procedure are the central issue in demonstrating histological features, the pretreatment and processing following staining also ma...
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Chapter
Reagents
A prerequisite for successful and reproducible staining is the use of the correct reagents. The composition, preparation, and storage qualities should be known. A detailed knowledge of the chemical and physica...
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Article
An investigation of new commercial samples of Methyl Green and Pyronin Y
New commercial samples of Methyl Green (Gurr Certistain), Pyronine G (Gurr Certistain) and Pyronin Y (Polysciences) have been investigated using spectrophotometry, thin layer chromatography and nuclear magneti...
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Article
Nucleic acid staining with the methyl green-pyronin method
We compared the staining obtained using commercially available pyronin Y samples with that obtained using pure pyronin Y in a standardized methyl green-pyronin procedure. In addition, the importance of the dye...
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Article
Standardized Methyl Green—Pyronin Y procedures using pure dyes
Fully standardized Methyl Green—Pyronin methods are presented. Pure Pyronin Y and purified Methyl Green or Ethyl Green are used either simultaneously in one dye bath or are used as a sequence of Pyronin Y and ...
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Article
Preparation and characterization of methyl green tetrafluoroborate
Commercial methyl green dyes were converted to tetrafluoroborate by means of NaBF4-solution, the compounds thus obtained were analytically pure. It was shown to be possible to distinguish between a “methyl” and a...
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Article
A Look at the Stationarity of 14 Quarterly U.S. Economic Time Series
Application of single series time series methods has become very popular, due, in particular, to the short term forecasting abilities of such models. This is particularly true of the Box-Jenkins linear models....
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Article
Induction of Leukaemia by 131I Treatment of Thyroid Carcinoma
The records of 194 patients with thyroid carcinoma treated with 131I, representing all cases thus treated in Denmark from 1948 to 1972, were reviewed. Two cases of myeloid leukaemia were found compared with 0·097...