Summary
Commercial methyl green dyes were converted to tetrafluoroborate by means of NaBF4-solution, the compounds thus obtained were analytically pure. It was shown to be possible to distinguish between a “methyl” and an “ethyl” compound by means of NMR spectroscopy. The dyes are stable in buffered aqueous solution, and in crystalline form. A spectrophotometric assay is proposed.
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Jakobsen, P., Andersen, A.P. & Lyon, H. Preparation and characterization of methyl green tetrafluoroborate. Histochemistry 81, 177–179 (1984). https://doi.org/10.1007/BF00490114
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DOI: https://doi.org/10.1007/BF00490114