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Article
Microfluorometric kinetic analysis of cathepsin B activity in single human thyroid follicular epithelial cells using image analysis and continuous monitoring
The activity of a cysteine proteinase, cathepsin B (EC 3.4.22.1), was determined in unfixed single human thyroid follicular epithelial cells at room temperature using an image analysis system. The formation of...
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Article
Elevated GLUT 1 level in crude muscle membranes from diabetic Zucker rats despite a normal GLUT 1 level in perineurial sheaths
Recently, we demonstrated that approximately 60 % of GLUT 1 in a crude membrane fraction of rat skeletal muscle originates from perineurial sheaths. To study the in vivo regulation of GLUT 1 expression in diff...
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Article
Metformin ameliorates diabetes but does not normalize the decreased GLUT 4 content in skeletal muscle of obese (fa/fa) Zucker rats
We studied the expression of the glucose transporter GLUT 4 in the soleus and red gastrocnemius muscles from obese, diabetic (fa/fa) Zucker rats compared to their lean littermates (Fa/-), with and without trea...
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Chapter
Tissue Processing: III. Fixation, General Aspects
Fixation is used in this text to denote a chemical treatment leading to immobilization and stabilization of tissue components. Displacement and extraction of native components should be minimal and, ideally, thos...
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Chapter
Tissue Processing: V. Embedding
Embedding of tissue enables it to withstand sectioning. One simple approach is to freeze the material and section it in a cryostat (Sects.11.2.2 and 11.3.1). The formation of ice has an immobilizing effect and...
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Chapter
Nucleic Acids
Some of the most important methods used for demonstrating nucleic acids are enumerated in Table 20.1, while Table 20.2 outlines a strategy for their identification using the classical histochemical reactions.
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Chapter
Carbohydrates
Histochemically demonstrable carbohydrates comprise homoglycans, heterogly-cans, and oligosaccharides bound to protein (glycoproteins) (cf. Sect.2.1.5). Homoglycans occur as glycogen in mammals and starch (amy...
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Chapter
11 Tissue Processing: II. Freezing
The purpose of freezing tissue immediately after sampling is to immobilize easily diffusible compounds.
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Chapter
Tissue Processing: IV. Applied Fixation
Although reactions with proteins are of overriding importance in tissue fixation (cf. Sect. 12.2), an appreciation of the reactions involving other macromolecules and lipids is also important. A selection of t...
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Chapter
Proteins
Some proteins, such as the fibre proteins elastin and collagen, can be identified directly by their tissue location and their physico-chemical properties. Similarly, actin and myosin can be recognized in stria...
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Chapter
Enzyme Histochemistry I: General Considerations
Enzymes are proteins or glycoproteins with selective, often specific, catalytic effects. They characteristically increase the rates of reactions by a factor of at least 107.
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Chapter
Enzyme Histochemistry II: Hydrolases
Hydrolases or hydrolytic enzymes catalyze the breakdown of substrates (e.g. esters, glycosides, and peptides) using water: $${\rm{AB + }}{{\rm...
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Chapter
Quantitation in Histochemistry
The purpose of this chapter is to provide an introduction to the techniques used in quantitative work and to cite key examples of staining procedures for which quantitation has been attempted. More detailed ac...
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Chapter
Enzyme Histochemistry III: Oxidoreductases
It is expedient to divide these enzymes into three groups:
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Dehydrogenases
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Article
Ultrastructure of germ cells and adjacent somatic cells correlated to initiation of meiosis in the fetal pig
The ultrastructure of female and male germ cells and associated somatic cells were studied in morphologically sex differentiated fetal pig gonads from day 27 to day 95 post insemination. Before meiosis starts ...
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Article
Nucleic acid staining with the methyl green-pyronin method
We compared the staining obtained using commercially available pyronin Y samples with that obtained using pure pyronin Y in a standardized methyl green-pyronin procedure. In addition, the importance of the dye...
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Article
Standardized Methyl Green—Pyronin Y procedures using pure dyes
Fully standardized Methyl Green—Pyronin methods are presented. Pure Pyronin Y and purified Methyl Green or Ethyl Green are used either simultaneously in one dye bath or are used as a sequence of Pyronin Y and ...
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Article
A quantitative cytochemical assay of β-galactosidase in single cultured human skin fibroblasts
A quantitative cytochemical method for the measurement of β-galactosidase activity in cultured human skin fibroblasts has been developed using 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside as the indigogenic s...
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Article
Use of epoxy resin slides for handling unfixed cryostat sections intended for histochemistry at the ultrastructural level
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Chapter
Histoenzymology of the Human Ovary: Dehydrogenases Directly Involved in Steroidogenesis
A close and complex functional relationship exists between the ovary, the hypothalamus and the pituitary gland. In the active reproductive period, this means that most of the different cell types in the human ...
