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An investigation of new commercial samples of Methyl Green and Pyronin Y

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Summary

New commercial samples of Methyl Green (Gurr Certistain), Pyronine G (Gurr Certistain) and Pyronin Y (Polysciences) have been investigated using spectrophotometry, thin layer chromatography and nuclear magnetic resonance, in addition to standardized simultaneous and sequential staining methods using purified Ethyl Green and pure Pyronin Y as reference dyes.

The Methyl Green was found to be Ethyl Green contaminated with Crystal Violet. It did not have any advantages compared with Ethyl Green supplied by American dye companies. The Pyronine G sample was Pyronin Y with a high dye content that gave good staining results when used with purified Ethyl Green. Pyronin Y from Polysciences was found to be essentially pure Pyronin Y.

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Author notes

  1. P. Jakobsen

    Present address: Ferrosan A/S Research Division, Sydmarken, DK-2860, Søborg, Denmark

Authors and Affiliations

  1. Department of Pathology, Hvidovre Hospital, University of Copenhagen, DK-2650, Hvidovre, Denmark

    H. Lyon

  2. Department of Chemistry, The Panum Institute, DK-2200, Copenhagen, Denmark

    P. Jakobsen

  3. Department of Medical Anatomy A, The Panum Institute, DK-2200, Copenhagen, Denmark

    P. Høyer

  4. School for Hospital Laboratory Technicians in Copenhagen, The Panum Institute, DK-2200, Copenhagen, Denmark

    A. P. Andersen

Authors
  1. H. Lyon
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  2. P. Jakobsen
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  3. P. Høyer
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  4. A. P. Andersen
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Lyon, H., Jakobsen, P., Høyer, P. et al. An investigation of new commercial samples of Methyl Green and Pyronin Y. Histochem J 19, 381–384 (1987). https://doi.org/10.1007/BF01680456

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  • DOI: https://doi.org/10.1007/BF01680456

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