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Article
Chromosomal location and reorganization of the 45S and 5S rDNA in theBrachyscome lineariloba complex (Asteraceae)
The chromosomal locations of the 45S (18S-5.8S-26S) and 5S ribosomal DNA in theBrachyscome lineariloba complex and two related species have been determined by the use of multicolor fluorescencein situ hybridizati...
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Article
Repetitive sequences: cause for variation in genome size and chromosome morphology in the genus Oryza
Large variation in genome size as determined by the nuclear DNA content and the mitotic chromosome size among diploid rice species is revealed using flow cytometry and image analyses. Both the total chromosoma...
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Article
Visual verification of close disposition between a rice A genome-specific DNA sequence (TrsA) and the telomere sequence
A rice A genome-specific tandem repeat sequence (TrsA) and telomeric nucleotide sequences, (TTTAGGG)n, were simultaneously detected by multicolor fluorescence in situ hybridization (McFISH) using rice prometaphas...
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Article
Physical map** of unique nucleotide sequences on identified rice chromosomes
A physical map** method for unique nucleotide sequences on specific chromosomal regions was developed combining objective chromosome identification and highly sensitive fluorescence in situ hybridisation (FI...
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Article
Quantitative chromosome map of the polyploid Saccharum spontaneum by multicolor fluorescence in situ hybridization and imaging methods
Somatic chromosomes of a wild relative of sugarcane (Saccharum spontaneum L.) anther culture-derived clone (AP 85-361, 2n=32) were identified and characterized by computer-aided imaging technology and molecula...
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Article
Bryophyte 5S rDNA was inserted into 45S rDNA repeat units after the divergence from higher land plants
The 5S ribosomal RNA genes (5S rDNA) are located independently from the 45S rDNA repeats containing 18S, 5.8S and 26S ribosomal RNA genes in higher eukaryotes. Southern blot and fluorescence in situ hybridization...
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Chapter
Smallness: gain and loss in plant chromosome research
Once, a good microscope was the pride of a laboratory in the field of cytology and cytogenetics. Now the situation seems to have completely changed. Biochemical and molecular biological equipment replace the p...
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Chapter
Rice Genome Research: An Alternative Approach Based on Molecular Cytology
A genome is a collection of chromosomes and visible chromosomes under a photomicroscope that provide a unique and effective approach to genome analysis. In situ hybridization (ISH) is one of the most effective me...
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Article
Site-specific accumulation of a LINE-like retrotransposon in a sex chromosome of the dioecious plant Cannabis sativa
Male-associated DNA sequences were analysed in hemp (Cannabis sativa L.), a dioecious plant with heteromorphic sex chromosomes. A male-associated DNA sequence in C. sativa (MADC1) and its flanking sequence encode...
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Chapter
Chromosome painting as a tool for rice genetics and breeding
Chromosome painting and genomic in situ hybridization (GISH) are both effective methods for basic genetic research and practical breeding. These methods were applied even in the typically small chromosomes of ric...
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Article
Chromosome painting as a tool for rice genetics and breeding
Chromosome painting and genomic in situ hybridization (GISH) are both effective methods for basic genetic research and practical breeding. These methods were applied even in the typically small chromosomes of ric...
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Article
Visualization of the terminal structure of rice chromosomes 6 and 12 with multicolor FISH to chromosomes and extended DNA fibers
High-resolution fluorescence in situ hybridization (FISH) on interphase and pachytene nuclei, and extended DNA fibers enabled microscopic distinction of DNA sequences less than a few thousands of base pairs apart...
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Article
Trends in site-number change of rDNA loci during polyploid evolution in Sanguisorba (Rosaceae)
To elucidate the evolutionary dynamics of rDNA site number in polyploid plants, we determined 5S and 18S-5.8S-26S rDNA sites for ten species of Sanguisorba (2n=14, 28, 56) and a single species of each of three o...
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Article
Chromosome maps of legumes
Legumes are of immense importance as food and feed, and for sustainable agriculture, due to their ability to fix nitrogen. Here, the chromosome maps of the legumes soybean (Glycine max), Lotus (Lotus japonicus), ...
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Article
Integration of cytogenetic and genetic linkage maps of Lotus japonicus, a model plant for legumes
To construct a high-resolution pachytene chromosome map, we used the chromosome image analyzing system version 3 and fluorescence in situ hybridization. Two ribosomal RNA genes (45S rDNA and 5S rDNA), two major t...
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Article
Open AccessStructural characterization of copia-type retrotransposons leads to insights into the marker development in a biofuel crop, Jatropha curcasL.
Recently, Jatropha curcas L. has attracted worldwide attention for its potential as a source of biodiesel. However, most DNA markers have demonstrated high levels of genetic similarity among and within jatropha p...
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Article
Identification and Characterization of Novel Gypsy-Type Retrotransposons in a Biodiesel Crop, Jatropha curcas L
Gypsy-type retrotransposons comprise a large proportion of the plant genome. Identification and determination of their chromosomal distribution could contribute to a better understanding of the r...
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Protocol
Image Analysis of DNA Fiber and Nucleus in Plants
Advances in cytology have led to the application of a wide range of visualization methods in plant genome studies. Image analysis methods are indispensable tools where morphology, density, and color play impor...
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Chapter
Flowering Genes and Homeotic Floral Gene Analysis in Jatropha
Jatropha, Jatropha curcas L., is a monoecious species, which forms unisexual flowers. The unisexual flowers are produced on the same inflorescence with 10 to 30-folds higher ratio of male flowers to female flower...
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Article
Molecular insights into the non-recombining nature of the spinach male-determining region
Spinach (Spinacia oleracea L.) is a dioecious plant with male heterogametic sex determination and homomorphic sex chromosomes (XY). The dioecism is utilized for producing commercial hybrid seeds, and hence unders...
