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    Article

    TaABI5, a wheat homolog of Arabidopsis thaliana ABA insensitive 5, controls seed germination

    Abscisic acid (ABA) response element (ABRE)-binding factors (ABFs) are basic region/leucine zipper motif (bZIP) transcription factors that regulate the expression of ABA-induced genes containing ABRE in their ...

    Shigeko Utsugi, Ikuo Ashikawa, Shingo Nakamura in Journal of Plant Research (2020)

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    Article

    A transgenic approach to controlling wheat seed dormancy level by using Triticeae DOG1-like genes

    Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we use...

    Ikuo Ashikawa, Masahiko Mori, Shingo Nakamura, Fumitaka Abe in Transgenic Research (2014)

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    Article

    A population genetics-based approach reveals candidate gene regions important in the determination of resistance specificity for the Pik family of rice blast resistance genes

    Allelic resistance (R) genes at the Pik locus for rice blast resistance consist of two genes, designated as the first gene and the second gene, which each have a nucleotide-binding site and a leucine-rich repeat....

    Ikuo Ashikawa, Ryuji Ishikawa in Journal of General Plant Pathology (2013)

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    The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast

    We report the isolation of Pi1, a gene conferring broad-spectrum resistance to rice blast (Magnaporthe oryzae). Using loss- and gain-of-function approaches, we demonstrate that Pi1 is an allele at the Pik locus. ...

    Lixia Hua, Jianzhong Wu, Caixia Chen, Weihuai Wu in Theoretical and Applied Genetics (2012)

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    Characterization of the rice blast resistance gene Pik cloned from Kanto51

    To study similar, but distinct, plant disease resistance (R) specificities exhibited by allelic genes at the rice blast resistance locus Pik/Pikm, we cloned the Pik gene from rice cultivar Kanto51 and compared it...

    Ikuo Ashikawa, Nagao Hayashi, Fumitaka Abe, Jianzhong Wu in Molecular Breeding (2012)

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    Article

    Haplotype diversity and molecular evolution of the rice Pikm locus for blast resistance

    Pikm-specific rice blast resistance is conferred by a combination of two genes that have a nucleotide-binding site and a leucine-rich repeat (LRR), Pikm1-TS and Pikm2-TS. To study the evolution of these genes, we...

    Ikuo Ashikawa, Jianzhong Wu, Takashi Matsumoto in Journal of General Plant Pathology (2010)

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    Article

    Visualization of the terminal structure of rice chromosomes 6 and 12 with multicolor FISH to chromosomes and extended DNA fibers

    High-resolution fluorescence in situ hybridization (FISH) on interphase and pachytene nuclei, and extended DNA fibers enabled microscopic distinction of DNA sequences less than a few thousands of base pairs apart...

    Nobuko Ohmido, Kyoko Kijima, Ikuo Ashikawa, J. Hans de Jong in Plant Molecular Biology (2001)

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    Article

    Surveying CpG methylation at 5′-CCGG in the genomes of rice cultivars

    We investigated the CpG methylation status of the sequence CCGG in the rice genome by using methylation-sensitive AFLP and subsequent Southern analyses with the isolated AFLP fragments as probes. CpGs located ...

    Ikuo Ashikawa in Plant Molecular Biology (2001)