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Article
TaABI5, a wheat homolog of Arabidopsis thaliana ABA insensitive 5, controls seed germination
Abscisic acid (ABA) response element (ABRE)-binding factors (ABFs) are basic region/leucine zipper motif (bZIP) transcription factors that regulate the expression of ABA-induced genes containing ABRE in their ...
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Article
A transgenic approach to controlling wheat seed dormancy level by using Triticeae DOG1-like genes
Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we use...
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Article
A population genetics-based approach reveals candidate gene regions important in the determination of resistance specificity for the Pik family of rice blast resistance genes
Allelic resistance (R) genes at the Pik locus for rice blast resistance consist of two genes, designated as the first gene and the second gene, which each have a nucleotide-binding site and a leucine-rich repeat....
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Article
The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast
We report the isolation of Pi1, a gene conferring broad-spectrum resistance to rice blast (Magnaporthe oryzae). Using loss- and gain-of-function approaches, we demonstrate that Pi1 is an allele at the Pik locus. ...
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Article
Characterization of the rice blast resistance gene Pik cloned from Kanto51
To study similar, but distinct, plant disease resistance (R) specificities exhibited by allelic genes at the rice blast resistance locus Pik/Pikm, we cloned the Pik gene from rice cultivar Kanto51 and compared it...
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Article
Haplotype diversity and molecular evolution of the rice Pikm locus for blast resistance
Pikm-specific rice blast resistance is conferred by a combination of two genes that have a nucleotide-binding site and a leucine-rich repeat (LRR), Pikm1-TS and Pikm2-TS. To study the evolution of these genes, we...
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Article
Visualization of the terminal structure of rice chromosomes 6 and 12 with multicolor FISH to chromosomes and extended DNA fibers
High-resolution fluorescence in situ hybridization (FISH) on interphase and pachytene nuclei, and extended DNA fibers enabled microscopic distinction of DNA sequences less than a few thousands of base pairs apart...
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Article
Surveying CpG methylation at 5′-CCGG in the genomes of rice cultivars
We investigated the CpG methylation status of the sequence CCGG in the rice genome by using methylation-sensitive AFLP and subsequent Southern analyses with the isolated AFLP fragments as probes. CpGs located ...