Abstract
Chromosome painting and genomic in situ hybridization (GISH) are both effective methods for basic genetic research and practical breeding. These methods were applied even in the typically small chromosomes of rice. This manuscript describes in detail, highly reproducible, complete protocols for chromosome painting and GISH in rice chromosomes. Examples of useful applications of these methods are also presented.
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Abbreviations
- McGISH:
-
Multi-color genomic in situ hybridization
- FISH:
-
Fluorescence in situ hybridization
- RIRE1:
-
Rice retrotransposon 1
- RFLP:
-
Restriction fragment length polymorphism
- TrsA:
-
Tandem repeat sequence A
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© 2001 Springer Science+Business Media Dordrecht
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Shishido, R., Ohmido, N., Fukui, K. (2001). Chromosome painting as a tool for rice genetics and breeding. In: Sharma, A.K., Sharma, A. (eds) Chromosome Painting. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0330-8_12
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DOI: https://doi.org/10.1007/978-94-010-0330-8_12
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3840-9
Online ISBN: 978-94-010-0330-8
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