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Article
Open AccessCRISPR imaging reveals chromatin fluctuation at the centromere region related to cellular senescence
The human genome is spatially and temporally organized in the nucleus as chromatin, and the dynamic structure of chromatin is closely related to genome functions. Cellular senescence characterized by an irreve...
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Article
Open AccessAuthor Correction: Molecular organization of recombinant human-Arabidopsis chromosomes in hybrid cell lines
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Protocol
Chromatin Immunostaining of Plant Nuclei
Recent evidence has demonstrated that specific epigenetic changes are also related to plant growth and development. Immunostaining enables the detection and characterization of chromatin modification, e.g., hi...
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Article
Open AccessMolecular organization of recombinant human-Arabidopsis chromosomes in hybrid cell lines
Although plants and animals are evolutionarily distant, the structure and function of their chromosomes are largely conserved. This allowed the establishment of a human-Arabidopsis hybrid cell line in which a ...
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Article
Surface structures consisting of chromatin fibers in isolated barley (Hordeum vulgare) chromosomes revealed by helium ion microscopy
The chromosome compaction of chromatin fibers results in the formation of the nucleosome, which consists of a DNA unit coiled around a core of histone molecules associated with linker histone. The compaction o...
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Article
Global epigenetic changes of histone modification under environmental stresses in rice root
Abiotic stresses are non-living factors with negative morphological and physiological effects on living organisms. Substantial evidence exists that gene expression changes during plant cell growth are regulate...
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Article
Open AccessCytogenetic and agronomic characterization of intergeneric hybrids between Saccharum spp. hybrid and Erianthus arundinaceus
In sugarcane (Saccharum spp. hybrid) breeding, introgression of useful genes via intergeneric hybridization is a powerful strategy for improving the crop productivity. Erianthus arundinaceus shows great potential...
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Article
Molecular insights into the non-recombining nature of the spinach male-determining region
Spinach (Spinacia oleracea L.) is a dioecious plant with male heterogametic sex determination and homomorphic sex chromosomes (XY). The dioecism is utilized for producing commercial hybrid seeds, and hence unders...
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Chapter
Flowering Genes and Homeotic Floral Gene Analysis in Jatropha
Jatropha, Jatropha curcas L., is a monoecious species, which forms unisexual flowers. The unisexual flowers are produced on the same inflorescence with 10 to 30-folds higher ratio of male flowers to female flower...
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Protocol
Image Analysis of DNA Fiber and Nucleus in Plants
Advances in cytology have led to the application of a wide range of visualization methods in plant genome studies. Image analysis methods are indispensable tools where morphology, density, and color play impor...
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Article
Identification and Characterization of Novel Gypsy-Type Retrotransposons in a Biodiesel Crop, Jatropha curcas L
Gypsy-type retrotransposons comprise a large proportion of the plant genome. Identification and determination of their chromosomal distribution could contribute to a better understanding of the r...
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Article
Open AccessStructural characterization of copia-type retrotransposons leads to insights into the marker development in a biofuel crop, Jatropha curcasL.
Recently, Jatropha curcas L. has attracted worldwide attention for its potential as a source of biodiesel. However, most DNA markers have demonstrated high levels of genetic similarity among and within jatropha p...
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Article
Integration of cytogenetic and genetic linkage maps of Lotus japonicus, a model plant for legumes
To construct a high-resolution pachytene chromosome map, we used the chromosome image analyzing system version 3 and fluorescence in situ hybridization. Two ribosomal RNA genes (45S rDNA and 5S rDNA), two major t...
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Article
Chromosome maps of legumes
Legumes are of immense importance as food and feed, and for sustainable agriculture, due to their ability to fix nitrogen. Here, the chromosome maps of the legumes soybean (Glycine max), Lotus (Lotus japonicus), ...
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Article
Trends in site-number change of rDNA loci during polyploid evolution in Sanguisorba (Rosaceae)
To elucidate the evolutionary dynamics of rDNA site number in polyploid plants, we determined 5S and 18S-5.8S-26S rDNA sites for ten species of Sanguisorba (2n=14, 28, 56) and a single species of each of three o...
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Article
Visualization of the terminal structure of rice chromosomes 6 and 12 with multicolor FISH to chromosomes and extended DNA fibers
High-resolution fluorescence in situ hybridization (FISH) on interphase and pachytene nuclei, and extended DNA fibers enabled microscopic distinction of DNA sequences less than a few thousands of base pairs apart...
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Article
Chromosome painting as a tool for rice genetics and breeding
Chromosome painting and genomic in situ hybridization (GISH) are both effective methods for basic genetic research and practical breeding. These methods were applied even in the typically small chromosomes of ric...
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Chapter
Chromosome painting as a tool for rice genetics and breeding
Chromosome painting and genomic in situ hybridization (GISH) are both effective methods for basic genetic research and practical breeding. These methods were applied even in the typically small chromosomes of ric...
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Article
Site-specific accumulation of a LINE-like retrotransposon in a sex chromosome of the dioecious plant Cannabis sativa
Male-associated DNA sequences were analysed in hemp (Cannabis sativa L.), a dioecious plant with heteromorphic sex chromosomes. A male-associated DNA sequence in C. sativa (MADC1) and its flanking sequence encode...
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Chapter
Smallness: gain and loss in plant chromosome research
Once, a good microscope was the pride of a laboratory in the field of cytology and cytogenetics. Now the situation seems to have completely changed. Biochemical and molecular biological equipment replace the p...
