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Article
Open AccessN-acetylneuraminic acid links immune exhaustion and accelerated memory deficit in diet-induced obese Alzheimer’s disease mouse model
Systemic immunity supports lifelong brain function. Obesity posits a chronic burden on systemic immunity. Independently, obesity was shown as a risk factor for Alzheimer’s disease (AD). Here we show that high-...
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Article
Blood and immune development in human fetal bone marrow and Down syndrome
Haematopoiesis in the bone marrow (BM) maintains blood and immune cell production throughout postnatal life. Haematopoiesis first emerges in human BM at 11–12 weeks after conception1,2, yet almost nothing is know...
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Article
COVID-19 tissue atlases reveal SARS-CoV-2 pathology and cellular targets
COVID-19, which is caused by SARS-CoV-2, can result in acute respiratory distress syndrome and multiple organ failure1–4, but little is known about its pathophysiology. Here we generated single-cell atlases of 24...
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Article
Skin-resident innate lymphoid cells converge on a pathogenic effector state
Tissue-resident innate lymphoid cells (ILCs) help sustain barrier function and respond to local signals. ILCs are traditionally classified as ILC1, ILC2 or ILC3 on the basis of their expression of specific tra...
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Article
Single-cell meta-analysis of SARS-CoV-2 entry genes across tissues and demographics
Angiotensin-converting enzyme 2 (ACE2) and accessory proteases (TMPRSS2 and CTSL) are needed for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cellular entry, and their expression may shed light...
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Article
snRNA-seq reveals a subpopulation of adipocytes that regulates thermogenesis
Adipose tissue is usually classified on the basis of its function as white, brown or beige (brite)1. It is an important regulator of systemic metabolism, as shown by the fact that dysfunctional adipose tissue in ...
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Article
Open AccessAuthor Correction: A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
A single-cell landscape of high-grade serous ovarian cancer
Malignant abdominal fluid (ascites) frequently develops in women with advanced high-grade serous ovarian cancer (HGSOC) and is associated with drug resistance and a poor prognosis1. To comprehensively characteriz...
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Article
Cumulus provides cloud-based data analysis for large-scale single-cell and single-nucleus RNA-seq
Massively parallel single-cell and single-nucleus RNA sequencing has opened the way to systematic tissue atlases in health and disease, but as the scale of data generation is growing, so is the need for comput...
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Article
Open AccessA single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors
Single-cell genomics is essential to chart tumor ecosystems. Although single-cell RNA-Seq (scRNA-Seq) profiles RNA from cells dissociated from fresh tumors, single-nucleus RNA-Seq (snRNA-Seq) is needed to prof...
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Article
Open AccessSingle cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation
Human iPSC-derived kidney organoids have the potential to revolutionize discovery, but assessing their consistency and reproducibility across iPSC lines, and reducing the generation of off-target cells remain ...
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Article
Decoding human fetal liver haematopoiesis
Definitive haematopoiesis in the fetal liver supports self-renewal and differentiation of haematopoietic stem cells and multipotent progenitors (HSC/MPPs) but remains poorly defined in humans. Here, using sing...
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Article
A revised airway epithelial hierarchy includes CFTR-expressing ionocytes
The airways of the lung are the primary sites of disease in asthma and cystic fibrosis. Here we study the cellular composition and hierarchy of the mouse tracheal epithelium by single-cell RNA-sequencing (scRN...
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Article
Epithelial microRNAs regulate gut mucosal immunity via epithelium–T cell crosstalk
Normal gut–immune system homeostasis requires interactions among epithelial cells, lymphocytes and host microflora. Ben-Neriah and colleagues show that defined microRNA expression in the gut mucosa is also imp...
