Bone Marrow Environment
Methods and Protocols
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Open AccessLymphopenia confers poorer prognosis in Myelodysplastic Syndromes with very low and low IPSS-M
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Article
What role for somatic mutations in systemic inflammatory and autoimmune diseases associated with myelodysplastic neoplasms and chronic myelomonocytic leukemias?
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Article
Open AccessWHIM Syndrome-linked CXCR4 mutations drive osteoporosis
WHIM Syndrome is a rare immunodeficiency caused by gain-of-function CXCR4 mutations. Here we report a decrease in bone mineral density in 25% of WHIM patients and bone defects leading to osteoporosis in a WHIM mo...
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Article
Genomic landscape of MDS/CMML associated with systemic inflammatory and autoimmune disease
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Protocol
Feeder-Free Differentiation Assay for Mouse Hematopoietic Stem and Progenitor Cells
Hematopoietic stem cells (HSCs) are responsible for replenishing immune cells and reside in bone marrow (BM) niches, which provide all cellular and molecular components required for their lifelong maintenance ...
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Protocol
Immunophenoty** of the Medullary B Cell Compartment In Mouse Models
B cell development is a stepwise process encompassing several B cell precursor stages that can be phenotypically distinguished, and that is taking place in the bone marrow in adults. Interestingly, within the ...
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Protocol
Culture, Expansion and Differentiation of Mouse Bone-Derived Mesenchymal Stromal Cells
Mesenchymal stem/stromal cells (MSCs) are multipotent adult cells that are present in several tissues including the bone marrow (BM), in which they can differentiate in a variety of cell types such as osteobla...
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Culture, Expansion and Differentiation of Human Bone Marrow Stromal Cells
Mesenchymal stromal cells (MSC) are a rare, heterogeneous and multipotent population that can be isolated from several tissues. MSC were originally discovered in the bone marrow and studied for their capacity ...
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Article
Open AccessFcγRIIb differentially regulates pre-immune and germinal center B cell tolerance in mouse and human
Several tolerance checkpoints exist throughout B cell development to control autoreactive B cells and prevent the generation of pathogenic autoantibodies. FcγRIIb is an Fc receptor that inhibits B cell activat...
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Protocol
Examination of Galectin-Induced Lattice Formation on Early B-Cell Development
Galectin-1 (GAL1) is a pre-B cell receptor (pre-BCR) ligand that induces pre-BCR clustering and leads to efficient pre-B cell proliferation and differentiation in the bone marrow. To study pre-BCR–GAL1 interac...
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Assessing T Follicular Helper Cell Function In Vivo: Antigen-Specific B Cell Response to Hapten and Affinity Maturation
The mechanisms controlling affinity maturation have been extensively studied over the last 20 years and the central role of T follicular helper cells (Tfh) in this process has now been clearly established. In ...
