Abstract
Galectin-1 (GAL1) is a pre-B cell receptor (pre-BCR) ligand that induces pre-BCR clustering and leads to efficient pre-B cell proliferation and differentiation in the bone marrow. To study pre-BCR–GAL1 interactions and its functional consequence on the early steps of the B cell development, we combine structural nuclear magnetic resonance (NMR) approaches and B cell biology techniques. NMR is applied to identify the residues involved in pre-BCR–GAL1 interactions by monitoring chemical shift perturbations when the complex is formed. This structural information is then used at the cellular level to target specifically the complex formation during GAL1-induced pre-BCR clustering and lattice formation, using immunofluorescence techniques. Moreover, an in vivo assay was set up to study the consequence of synapse formation on the early steps of B cell development.
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Mancini, S.J.C., Elantak, L., Boned, A., Espéli, M., Guerlesquin, F., Schiff, C. (2015). Examination of Galectin-Induced Lattice Formation on Early B-Cell Development. In: Stowell, S., Cummings, R. (eds) Galectins. Methods in Molecular Biology, vol 1207. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1396-1_11
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DOI: https://doi.org/10.1007/978-1-4939-1396-1_11
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1395-4
Online ISBN: 978-1-4939-1396-1
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