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Feeder-Free Differentiation Assay for Mouse Hematopoietic Stem and Progenitor Cells

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Bone Marrow Environment

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2308))

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Abstract

Hematopoietic stem cells (HSCs) are responsible for replenishing immune cells and reside in bone marrow (BM) niches, which provide all cellular and molecular components required for their lifelong maintenance and differentiation. Although HSCs have been extensively analyzed and characterized, their ex vivo expansion, which constitutes a promising approach for therapeutic development in regenerative medicine, remains challenging. Here, we describe an original in vitro system allowing to quantify by flow cytometry the differentiation of mouse HSCs into lineage-primed multipotent hematopoietic progenitors (MPPs) in a cytokine-supplemented feeder-free medium.

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Acknowledgments

This work was supported by the ANR grant PRC 17-CE14-0019; the INCa agency under the program PRT-K 2017; a PhD fellowship from the Fondation pour la Recherche Médicale, and a fourth year PhD fellowship from the Ligue Nationale Contre le Cancer.

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Authors and Affiliations

  1. Université de Paris, Institut de Recherche Saint-Louis, EMiLy, INSERM U1160, Paris, France

    Vincent Rondeau

  2. Université de Paris, Institut de Recherche Saint-Louis, OPALE Carnot Institute, EMiLy, INSERM U1160, Paris, France

    Marion Espéli & Karl Balabanian

Authors
  1. Vincent Rondeau
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  2. Marion Espéli
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  3. Karl Balabanian
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Corresponding authors

Correspondence to Vincent Rondeau or Karl Balabanian .

Editor information

Editors and Affiliations

  1. Université de Paris, Institut de Recherche Saint Louis, OPALE Carnot Institute, EMiLy, INSERM U1160, Paris, France

    Marion Espéli

  2. Université de Paris, Institut de Recherche Saint Louis, OPALE Carnot Institute, EMiLy, INSERM U1160, Paris, France

    Karl Balabanian

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Rondeau, V., Espéli, M., Balabanian, K. (2021). Feeder-Free Differentiation Assay for Mouse Hematopoietic Stem and Progenitor Cells. In: Espéli, M., Balabanian, K. (eds) Bone Marrow Environment. Methods in Molecular Biology, vol 2308. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1425-9_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1425-9_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1424-2

  • Online ISBN: 978-1-0716-1425-9

  • eBook Packages: Springer Protocols

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