22 Result(s)
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Jian Wen Wang
Biotechnology
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Article
Bamboo polysaccharides elicit hypocrellin A biosynthesis of a bambusicolous fungus Shiraia sp. S9
Hypocrellin A (HA), a fungal perylenequinone from bambusicolous Shiraia species, is a newly developed photosensitizer for photodynamic therapy in cancer and other infectious diseases. The lower yield of HA is an ...
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Article
Open AccessEffects of branched-chain amino acids on Shiraia perylenequinone production in mycelium cultures
Perylenequinones from Shiraia fruiting bodies are excellent photosensitizers and widely used for anti-cancer photodynamic therapy (PDT). The lower yield of Shiraia perylenequinones becomes a significant bottlenec...
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Article
Open AccessContrasting regulation of live Bacillus cereus No.1 and its volatiles on Shiraia perylenequinone production
Fungal perylenequinones (PQs) are a class of photoactivated polyketide mycotoxins produced by plant-associated fungi. Hypocrellins, the effective anticancer photodynamic therapy (PDT) agents are main bioactive...
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Article
Open AccessThe signaling role of extracellular ATP in co-culture of Shiraia sp. S9 and Pseudomonas fulva SB1 for enhancing hypocrellin A production
Adenosine 5′-triphosphate (ATP) plays both a central role as an intracellular energy source, and a crucial extracellular signaling role in diverse physiological processes of animals and plants. However, there ...
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Article
Open AccessNitric oxide donor sodium nitroprusside-induced transcriptional changes and hypocrellin biosynthesis of Shiraia sp. S9
Nitric oxide (NO) is a ubiquitous signaling mediator in various physiological processes. However, there are less reports concerning the effects of NO on fungal secondary metabolites. Hypocrellins are effective...
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Article
Open AccessInducing perylenequinone production from a bambusicolous fungus Shiraia sp. S9 through co-culture with a fruiting body-associated bacterium Pseudomonas fulva SB1
Fungal perylenequinonoid (PQ) pigments from Shiraia fruiting body have been well known as excellent photosensitizers for medical and agricultural uses. The fruiting bodies are colonized by a diverse bacterial com...
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Article
Open AccessEngineering of 3-ketosteroid-∆1-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates
Biosynthesis of steroidal drugs is of great benefit in pharmaceutical manufacturing as the process involves efficient enzymatic catalysis at ambient temperature and atmospheric pressure compared to chemical sy...
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Chapter and Conference Paper
Research Progress of Aldehyde Ketone Reductase for Asymmetric Catalysis of Chiral Compounds
Aldehyde ketone reductase (AKR) belongs to the redox enzyme superfamily. The aldo-keto reductase (AKR) protein superfamily contains over 190 members that fall into 16 families. Its biological catalysis in the ...
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Chapter and Conference Paper
Research Progress of Squalene Synthase on Function and Application
Squalene (SQ) is an unsaturated triterpene which is formed by six linked isoprenes, it is a key intermediate product in the metabolic pathway of cholesterol synthesis. The synthetic process of cholesterol is a...
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Article
Open AccessBiochemical analysis and the preliminary crystallographic characterization of d-tagatose 3-epimerase from Rhodobacter sphaeroides
d-Tagatose 3-epimerase epimerizes d-fructose to yield d-psicose, which is a rare sugar that exists in small quantities in nature and is difficult to synthesize chemically. We aim to ex...
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Article
Transcriptomic responses involved in enhanced production of hypocrellin A by addition of Triton X-100 in submerged cultures of Shiraia bambusicola
The addition of surfactant is a useful strategy to enhance the product yield in submerged fermentation process. In this study, we sought to explore the mechanism for the elicitation of Triton X-100 on producti...
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Article
Transcriptome sequencing and development of novel genic SSR markers for Dendrobium officinale
Dendrobium officinale is a critically endangered and valuable medicinal herb. In this study, two transcriptome libraries were constructed from 2-year-old stems of two cultivated varieties...
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Article
Multienzymatic cascade synthesis of fucosyloligosaccharide via a two-step fermentation strategy in Escherichia coli
To achieve multienzymatic cascade synthesis of fucosyl oligosaccharide from d-mannose by two-step fermentation pathway in Escherichia coli.
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Article
PEGylation of cytochrome c at the level of lysine residues mediated by a microbial transglutaminase
To establish a method for microbial transglutaminase (mTG)-mediated PEGylation of proteins at the level of lysine (Lys) residues.
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Chapter
Effective Elicitors and Process Strategies for Enhancement of Secondary Metabolite Production in Hairy Root Cultures
This chapter reviews the various biotic and abiotic elicitors applied to hairy root cultures and their stimulating effects on the accumulation of secondary metabolites. Elicitors generally refer to the agents ...
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Article
Nitric oxide elicitation for secondary metabolite production in cultured plant cells
Nitric oxide (NO) is an important signal molecule in stress responses. Accumulation of secondary metabolites often occurs in plants subjected to stresses including various elicitors or signal molecules. NO has...
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Article
Tanshinone biosynthesis in Salvia miltiorrhiza and production in plant tissue cultures
Salvia miltiorrhiza Bunge (Lamiaceae) root, generally called Danshen, is an important herb in Chinese medicine widely used for treatment of cardiovascular diseases. Diterpenoid tanshinons are major bioactive cons...
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Article
Stimulation of artemisinin synthesis by combined cerebroside and nitric oxide elicitation in Artemisia annua hairy roots
This work examined the accumulation of artemisinin and related secondary metabolism pathways in hairy root cultures of Artemisia annua L. induced by a fungal-derived cerebroside (2S,2′R,3R,3′E,4E,8E)-1-O-β-d-gluc...
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Article
Involvement of nitric oxide in cerebroside-induced defense responses and taxol production in Taxus yunnanensis suspension cells
This work was to characterize the generation of nitric oxide (NO) in Taxus yunnanensis cells induced by a fungal-derived cerebroside and the signal role of NO in the elicitation of plant defense responses and tax...
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Article
Improved artemisinin accumulation in hairy root cultures of Artemisia annua by (22S, 23S)-homobrassinolide
When (22S, 23S)-homobrassinolide (SSHB) was added at 1 μg l−1 to hairy root cultures of Artemisia annua, the production of artemisinin reached to 14 mg l−1, an increment of 57% over the control. SSHB treatment le...
