21 Result(s)
within
Irving L. Weissman
Life Sciences
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Article
Open AccessCell-type-specific aging clocks to quantify aging and rejuvenation in neurogenic regions of the brain
The diversity of cell types is a challenge for quantifying aging and its reversal. Here we develop ‘aging clocks’ based on single-cell transcriptomics to characterize cell-type-specific aging and rejuvenation....
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Article
Open AccessRadiotherapy in combination with CD47 blockade elicits a macrophage-mediated abscopal effect
Radiation therapy is a mainstay of cancer treatment but does not always lead to complete tumor regression. Here we combine radiotherapy with blockade of the ‘don’t-eat-me’ cell-surface molecule CD47 in small c...
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Article
The pleiotropic benefits of statins include the ability to reduce CD47 and amplify the effect of pro-efferocytic therapies in atherosclerosis
The pleiotropic benefits of statins may result from their impact on vascular inflammation. The molecular process underlying this phenomenon is not fully elucidated. In the present study, RNA-sequencing designe...
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Article
De novo mutations in mitochondrial DNA of iPSCs produce immunogenic neoepitopes in mice and humans
The utility of autologous induced pluripotent stem cell (iPSC) therapies for tissue regeneration depends on reliable production of immunologically silent functional iPSC derivatives. However, rejection of auto...
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Article
Regenerating the field of cardiovascular cell therapy
The retraction of >30 falsified studies by Anversa et al. has had a disheartening impact on the cardiac cell therapeutics field. The premise of heart muscle regeneration by the transdifferentiation of bone mar...
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Article
Isolation and functional assessment of mouse skeletal stem cell lineage
A flow cytometry–based approach using eight surface markers is used to distinguish cells of the skeletal stem cell lineage. Renal subcapsular transplantation and in vitro colony-formation assays are also describe...
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Article
Computational correction of index switching in multiplexed sequencing libraries
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Protocol
Immunogenicity of In Vitro Maintained and Matured Populations: Potential Barriers to Engraftment of Human Pluripotent Stem Cell Derivatives
The potential to develop into any cell type makes human pluripotent stem cells (hPSCs) one of the most promising sources for regenerative treatments. Hurdles to their clinical applications include (1) formatio...
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Article
Identification and prospective isolation of a mesothelial precursor lineage giving rise to smooth muscle cells and fibroblasts for mammalian internal organs, and their vasculature
Fibroblasts and smooth muscle cells (FSMCs) are principal cell types of connective and adventitial tissues that participate in the development, physiology and pathology of internal organs, with incompletely de...
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Article
Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells
Drukker and colleagues differentiated human embryonic stem (ES) cells for 3 days and screened the cells for labeling by >400 antibodies. They identified cell-surface markers expressed on four classes of early ...
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Article
Tracking single hematopoietic stem cells in vivo using high-throughput sequencing in conjunction with viral genetic barcoding
Heterogeneity within populations of stem cells, cancer cells or other cell types of interest presents a formidable barrier to analysis. Lu et al. use viral barcoding and high-throughput sequencing to track the di...
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Article
An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells
Using cells derived from human pluripotent stem cells for therapeutic applications carries a risk that rare undifferentiated cells in the transplant will give rise to teratomas. Tang et al. identify a new antigen...
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Article
In vivo evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood
Establishment of robust xenograft models is critical to studying human hematopoiesis in a physiologic setting. Using a recently developed immunodeficient mouse strain, we have established long-term multilineag...
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Article
Hematopoietic stem cells
This review focuses on the genomics of mouse hematopoiesis but also draws parallels to other systems and discusses issues common to the analysis of rare populations such as stem cells. As examples from the mou...
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Article
Construction and Characterization of Large-Insert Genomic Libraries (BAC and Fosmid) from the Ascidian Botryllus schlosseri and Initial Physical Map** of a Histocompatibility Locus
The colonial protochordate Botryllus schlosseri is genetically manipulable and represents a potential model organism for a variety of biological disciplines, including immunology, stem cell biology and developmen...
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Formation and Differentiation of Leukocytes
Inflammatory responses often involve the selective accumulation in tissues of complex mixtures of leukocytes. In order to understand the processes governing migration and accumulation of mature leukocytes, it ...
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Chapter
T-Cell Development from Hematopoietic Stem Cells
The central cells of the immune system include three major populations of lymphocytes with distinct antigen recognition receptors: T-cells, B-cells, and natural killer (NK) cells. All lymphocyte populations, a...
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Chapter
Retreat Growth in the Ascidian Botryllus Schlosseri: A Consequence of Nonself Recognition
In the genus Botryllus fusion between genetically distinct individuals is controlled by a single genetic locus (or haplotype) with multiple codominantly expressed alleles. Colonies which do not share an allele at...
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Chapter
Speculations on the Relationships of Two Botryllus Allo-Recognition Reactions—Colony Specificity and Resorption—To Vertebrate Histocompatibility
The objective of this essay is to consider functions of the vertebrate major histocompatibility complex (MHC), especially in comparison to allorecognition in colonial tunicates. Elsewhere Burnet (1971), and ou...
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Lymphocyte Homing Receptors, Ubiquitin, and Cell Surface Proteins
The immune system, unlike most organ systems that are consolidated in one anatomic location, is dispersed over an entire organism. It exists as circulating elements in the blood, through which it gains access ...
