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Article
Evidence for mutations in the structural gene for homocitrate synthase in Saccharomycopsis lipolytica
Eight strains devoid of homocitrate synthase activity were found among lysine requiring mutants of the yeast Saccharomycopsis lipolytica. Genetic analysis of these strains showed that they were all affected at th...
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Article
Replication and recombination of 2-µm DNA in Schizosaccharomyces pombe
Any one of the inverted sequences present on the 2-µm DNA from Saccharomyces cerevisiae can promote replication of chimeric plasmids in Schizosaccharomyces pombe. When however a complete 2-µm molecule is present ...
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Article
LEU2 directed expression of β-galactosidase activity and phleomycin resistance in Yarrowia lipolytica
The nucleotide sequence of a 968 by DNA fragment spanning the promoter and the 5′ upstream sequence of the LEU2 coding sequence of the yeast Yarrowia lipolytica has been determined. A LEU2:lacZ gene fusion has be...
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Article
Cloning of the LYS5 gene encoding saccharopine dehydrogenase from the yeast Yarrowia lipolytica by target integration
A Yarrowia lipolytica yeast gene bank has been constructed in E. coli in an integrative plasmid vector containing the homologous LEU2 gene, and used to transform a leu2 lys5 yeast strain. The LYS5 gene encoding s...
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Article
Expression of invertase activity in Yarrowia lipolytica and its use as a selective marker
Few selective markers are available for the transformation of the industrial yeast Yarrowia lipolytica, and those that are require the use of specialized hosts (e.g., auxotrophs, antibiotic sensitive). To enable ...
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Article
The yeast Yarrowia lipolytica has two, functional, signal recognition particle 7S RNA genes
Cells containing a deletion of either the SCR1 or SCR2 genes, which code for the 7SL RNA component of the signal recognition particle (SRP) homologue, were found to be viable. Two independent approaches demonstra...
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Article
Cloning of the mating-type gene MATA of the yeast Yarrowia lipolytica
The mating type gene MA TA of the dimorphic yeast Yarrowia lipolytica was cloned. The strategy used was based on the presumed function of this gene in the induction of sporulation. A diploid strain homozygous for...
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Article
Hyperthermostable Variants of a Highly Thermostable Alpha-Amylase
Genetic screening at temperatures between 70–80°C far exceeds the range of growth of most bacteria, and is not applicable to isolate easily thermostable protein variants. We describe a temperature shift protoc...
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Article
Chromosomal polymorphism of the yeast Yarrowia lipolytica and related species: electrophoretic karyoty** and hybridization with cloned genes
Significant differences in electrophoretic karyoty** patterns were found among 27 strains of Y. lipolytica. Twenty-one of these strains were classified into four groups of similar karyotypes while six strains s...
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Article
LYC1 is the structural gene for lysine N-6-acetyl transferase in yeast
In the yeastYarrowia lipolytica, theLYC1 locus controls the first step of the lysine degradation pathway which is catalyzed by lysine N-6-acetyl transferase (LAT). This gene was cloned by complementation of thely...
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Article
Multiple-copy integration in the yeast Yarrowia lipolytica
Using an EcoRI-BglII fragment of the G unit of the rDNA of Y. lipolytica and a set of 11 deletions in the URA3 promoter, we have constructed several plasmids to test gene amplification in the rDNA. These plasmids...
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Article
Molecular cloning of Rab-related genes in the yeast Yarrowia lipolytica. Analysis of RYL1, an essential gene encoding a SEC4 homologue
Small GTP-binding proteins of the Rab family are involved in the vesicular traffic inside eukaryotic cells. A gene library from the yeast Yarrowia lipolytica was screened with an oligonucleotide deduced from a hi...
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Chapter
Yarrowia lipolytica
Interest in Candida lipolytica (Harrison) Diddens et Lodder 1942 initially arose from its rather uncommon physiological characteristics. Strains of this species were more often isolated from lipid- or protein-con...
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Article
Cloning the Yarrowia lipolytica homologue of the Saccharomyces cerevisiae SEC62 gene
Yarrowia lipolytica SEC62 cDNA was cloned by functional complementation of a thermo-sensitive sec62 Saccharomyces cerevisiae mutant strain. The Y. lipolytica SEC62 promoter region was amplified by the inverse po...
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Article
Open AccessUse of specific DNA probes for the rapid characterization of yeasts isolated from complex biotopes
We describe a rapid method developed to identify yeast species commonly found during cheese ripening. It is based on the isolation of species-specific sequences and their hybridization with coarse preparations...
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Chapter
Functional genetics of Yarrowia lipolytica
The yeast Yarrowia lipolytica has been used for industrial bioconversions since the late 1960’s, mainly by the food and chemical industries. The most important process is presently the production of citric acid f...
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Article
Chromosomal polymorphism in the yeast species Debaryomyces hansenii
Pulse field gel electrophoresis karyotypes of 41 strains of the genus Debaryomyces, including 35 strains confirmed as D. hansenii species by D1/D2 ribosomal DNA sequence analysis, were performed. Electrophoretic ...
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Article
Chromosomal polymorphism in the yeast species Debaryomyces hansenii
Pulse field gel electrophoresis karyotypes of 41 strains of the genus Debaryomyces, including 35 strains confirmed as D. hansenii species by D1/D2 ribosomal DNA sequence analysis, were performed. Electrophoretic ...
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Article
Genome evolution in yeasts
Identifying the mechanisms of eukaryotic genome evolution by comparative genomics is often complicated by the multiplicity of events that have taken place throughout the history of individual lineages, leaving...
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Article
Functional Analysis of the MATB Mating-Type Idiomorph of the Dimorphic Fungus Yarrowia lipolytica
The whole MATA cassette from Yarrowia lipolytica, a dimorphic fungus, was replaced by the URA3 gene through a double homologous recombination. This MAT-less strain lost its mate capacity with A or B Y. lipolytica
