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Article
CRAT links cholesterol metabolism to innate immune responses in the heart
Chronic inflammation is associated with increased risk and poor prognosis of heart failure; however, the precise mechanism that provokes sustained inflammation in the failing heart remains elusive. Here we rep...
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Article
Untargeted metabolomics analysis of ischemia–reperfusion-injured hearts ex vivo from sedentary and exercise-trained rats
The effects of exercise on the heart and its resistance to disease are well-documented. Recent studies have identified that exercise-induced resistance to arrhythmia is due to the preservation of mitochondrial...
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Article
Diggin′ on U(biquitin): A Novel Method for the Identification of Physiological E3 Ubiquitin Ligase Substrates
The ubiquitin–proteasome system (UPS) plays a central role in maintaining protein homeostasis, emphasized by a myriad of diseases that are associated with altered UPS function such as cancer, muscle-wasting, a...
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Protocol
Reconstitution of CHIP E3 Ubiquitin Ligase Activity
CHIP, the carboxyl-terminus of Hsp70 interacting protein, is both an E3 ubiquitin ligase and an Hsp70 co-chaperone and is implicated in the degradation of cytosolic quality control and numerous disease substra...
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Article
Breast cancer quality control
Tumorigenesis is regulated by several mechanisms including signalling, transcription and DNA replication. Now a cytoplasmic protein quality-control pathway is implicated in the suppression of breast cancer cel...
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Article
Structural basis of nucleotide exchange and client binding by the Hsp70 cochaperone Bag2
Bag2 acts as a nucleotide-exchange factor for Hsp70 and also binds misfolded substrates. Now structural work reveals that Bag2 promotes nucleotide exchange via a mechanism distinct from other Hsp70 nucleotide-...
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Chapter
Up and Down Regulation of the Stress Response by the Co-Chaperone Ubiquitin Ligase CHIP
Following exposure to environmental insults, the cells in most tissues dramatically increase the production of a group of proteins that are collectively known as “heat shock” or stress proteins (Parsell and Lindq...
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Book
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Protocol
Cystic Fibrosis Transmembrane Conductance Regulator as a Model Substrate to Study Endoplasmic Reticulum Protein Quality Control in Mammalian Cells
Components of the ubiquitin-proteasome system function on the surface of the endoplasmic reticulum (ER) to select misfolded proteins for degradation. Herein we describe methods that allow for the study of the ...
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Article
The co-chaperone CHIP regulates protein triage decisions mediated by heat-shock proteins
To maintain quality control in cells, mechanisms distinguish among improperly folded peptides, mature and functional proteins, and proteins to be targeted for degradation. The molecular chaperones, including h...
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Article
The Hsc70 co-chaperone CHIP targets immature CFTR for proteasomal degradation
The folding of both wild-type and mutant forms of the cystic-fibrosis transmembrane-conductance regulator (CFTR), a plasma-membrane chloride-ion channel, is inefficient1,2,3,4. Most nascent CFTR is retained in th...
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Article
Angiotensin II induces gene transcription through cell-type-dependent effects on the nuclear factor-κB (NF-κB) transcription factor
The vasopressor octapeptide, angiotensin II (Ang II), exerts homeostatic responses in cardiovascular tissues, including the heart, blood vessel wall, adrenal cortex and liver (a major source of circulating pla...
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Chapter
Angiotensin II induces gene transcription through cell-type-dependent effects on the nuclear factor-кB (NF-кB) transcription factor
The vasopressor octapeptide, angiotensin II (Ang II), exerts homeostatic responses in cardiovascular tissues, including the heart, blood vessel wall, adrenal cortex and liver (a major source of circulating pla...
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Article
Genomic structure of the human KDR/flk-1 gene