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145 Result(s)
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Article
Dominance and non-complementation among pink-adenineless mutants of Schizophyllum commune involving two discrete genes
In Schizophyllum commune, adenineless mutations conferring pink color fell into two loosely linked loci, ad-4 and ad-7. The mutations were tested for dominance and complementation in dikaryons, and several of the...
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Article
Heptose-deficient mutants ofEscherichia coli K12 deficient in up to three major outer membrane proteins
Mutants ofEscherichia coli K12, deficient in up to three major outer membrane proteinsb,c andd have been constructed. Mutants that lack the lipopolysaccharide sugar heptose are deficient in proteinb. All heptose-...
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Article
Conjugation deficient E. coli K12 F- mutants with heptose-less lipopolysaccharide
Two F- mutants deficient in conjugation with F-type donors are isolated and characterized. Phenotypically, these mutants are similar; they have heptose-less lipopolysaccharide and lack some outer membrane protein...
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Influence of cultural conditions and mutations on the composition of the outer membrane proteins ofEscherichia coli
VariousEscherichia coli strains differ in the composition of their major outer membrane proteins. However, allE. coli K12 strains tested possess the same major outer membrane proteinsa, b, c andd although quantit...
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Article
In vitro transcription of three different ribosomal RNA cistrons of E. coli; heterogeneity of control regions
Ribosomal RNA synthesis from three different rRNA cistrons of E. coli, located on different phage DNAs was compared and found to have the same characteristics as regards chain length, salt and temperature depende...
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Article
Genetic map** of the lexC-113 mutation
A mutation, previously designated lex-113 and suspected to be situated in the lexA locus, has been positioned by transductional studies to a unique site on the chromosome of E. coli B separate from the lexA102 an...
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Pores in the outer membrane of Escherichia coli K12
Mutants lacking outer membrane proteins were studied in order to investigate the role of these proteins in the functioning of aqueous pores through the outer membrane. Protein b is involved in the functioning ...
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Article
Genetics and biochemistry of the peptidoglycan-associated proteins b and c of Escherichia coli K12
To collect information on synthesis and regulation of the peptidoglycan-associated pore-forming outer membrane proteins b and c, mutants resistant to phages Mel and TuIa were analyzed. Genetic analysis showed ...
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meoA is the structural gene for outer membrane protein c of Escherichia coli K12
The isolation and characterization of two mutants of Escherichia coli K12 with an altered outer membrane protein c is described. The first mutant, strain CE1151, was isolated as a bacteriophage Mel resistant stra...
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Article
Specific stimulation of ribosomal RNA synthesis in E. coli by a protein factor
Ribosomal RNA synthesis in a purified system is stimulated by a crude protein fraction prepared from E. coli. The positive effector which is not associated with RNA polymerase, nor is the sigma factor, increases ...
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Article
A mutation in the RNA polymerase β′ subunit causing depressed ribosomal RNA synthesis in Escherichia coli
Macromolecular synthesis in an Escherichia coli mutant with a temperature-sensitive β′ subunit of RNA polymerase was analysed. At the non-permissive temperature ribosomal RNA synthesis is strongly reduced while m...
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Article
Cloning of ompF, the structural gene for an outer membrane pore protein of E. coli K12: Physical localization and homology with the phoE gene
The ompF gene of Escherichia coli K12, which is the structural gene for an outer membrane pore protein has been cloned into the multicopy plasmid vector pACYC184. Expression of the cloned ompF gene results in ove...
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Article
Phenotypic expression in vivo and transforming activity in vitro: Two related functions of folded bacterial chromosomes
Nucleoids prepared by gentle lysis of non-complementing diploid cells resulting from Bacillus subtilis protoplast fusion have been used to transform competent cultures of appropriate recipient strains. The yields...
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Article
Suppression of the lexC (ssbA) mutation of Escherichia coli by a mutant of bacteriophage P1
A new mutant of bacteriophage P1 designated lxc that suppresses the phenotype of lexC and ssbA mutants of Escherichia coli was isolated and characterized. The properties of lexC mutants suppressed by the lxc muta...
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Article
Thermal activation of hexosaminidase A in a genetic compound with Tay-Sachs disease
Increase in total hexosaminidase activity has been observed during heat treatment of serum and leukocyte specimens from a 1-year-old boy with cherry-red spot and severe and progressive mental and motor deterio...
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Article
Analyse génétique de sacB, gène de structure d'une enzyme secrétée, la lévane-saccharase de Bacillus subtilis Marburg
The structural gene sacB encoding B. subtilis levansucrase, a secreted enzyme, expresses in E. coli. E. coli hosts of the sacB gene are poisoned by sucrose. This property allowed a powerful selection of mutants a...
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Mode of organization of lipid aggregates: A conformational analysis
A computational approach is used to predict the mode of organization of lipid molecules. The calculated aggregate states are fully compatible with the lipid phase preference: bilayers for dipalmitoylphosphatid...
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Article
Cloning of phoM, a gene involved in regulation of the synthesis of phosphate limitation inducible proteins in Escherichia coli K12
Like the synthesis of alkaline phosphatase, the synthesis of outer membrane PhoE protein is shown to be dependent on the phoM gene product in phoR mutants of E. coli K12. This phoM gene has been cloned into the m...
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Gene encoding a hybrid OmpF-PhoE pore protein in the outer membrane of Escherichia coli K12
To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2–73 of the mature PhoE protein is replaced...
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Article
Characteristics of site variation among clones of the 340-base pair, tandemly repeated EcoR1 family of human DNA
Twenty-four clones of EcoR1-restricted, 340-base pair (bp) DNA derived from human DNA have been sequenced and compared to a published consensus sequence for this family. No two clones were found to have identical...