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Article
Open AccessHow Streptococcus suis escapes antibiotic treatments
Streptococcus suis is a zoonotic agent that causes sepsis and meningitis in pigs and humans. S. suis infections are responsible for large economic losses in pig production. The lack of effective vaccines to preve...
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Article
Open AccessFratricide activity of MafB protein of N. meningitidis strain B16B6
Neisseria meningitidis is an inhabitant of the mucosal surfaces of the human nasopharynx. We recently demonstrated that the secreted meningococcal Two-partner secretion protein A (TpsA...
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Article
Open AccessUnearthing the genomes of plant-beneficial Pseudomonas model strains WCS358, WCS374 and WCS417
Plant growth-promoting rhizobacteria (PGPR) can protect plants against pathogenic microbes through a diversity of mechanisms including competition for nutrients, production of antibiotics, and stimulation of t...
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Chapter
Microbial Cell Surfaces and Secretion Systems
Microbial cell surfaces, surface-exposed organelles, and secreted proteins are important for the interaction with the environment, including adhesion to hosts, protection against host defense mechanisms, nutri...
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Protocol
Experimental Methods for Studying the BAM Complex in Neisseria meningitidis
Neisseria meningitidis is a human pathogen. It is intensively studied for host–pathogen interactions and vaccine development. However, its favorable growth properties, genetic accessibility, and small genome siz...
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Article
Open AccessDomain exchange at the 3’ end of the gene encoding the fratricide meningococcal two-partner secretion protein A
Two-partner secretion systems in Gram-negative bacteria consist of an outer membrane protein TpsB that mediates the secretion of a cognate TpsA protein into the extracellular milieu. TpsA proteins have diverse...
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Protocol
Assembly of Bacterial Outer Membrane Proteins
Various methods that are routinely used to study the subcellular localization of membrane proteins in wild-type Gram-negative bacteria fall short in genetic studies addressing the biogenesis of outer membrane ...
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Protocol
TMT Labelling for the Quantitative Analysis of Adaptive Responses in the Meningococcal Proteome
In addition to standard gel-based proteomic approaches, gel-free approaches using isobaric label reagents, such as Tandem Mass Tags (TMT), provide a straightforward method for studying adaptations in microbial...
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Article
Open AccessBiogenesis of β-barrel membrane proteins in bacteria and eukaryotes: evolutionary conservation and divergence
Membrane-embedded β-barrel proteins span the membrane via multiple amphipathic β-strands arranged in a cylindrical shape. These proteins are found in the outer membranes of Gram-negative bacteria, mitochondria...
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Article
Structure of a membrane-based steric chaperone in complex with its lipase substrate
Secretion via the type II secretion pathway in Gram-negative bacteria often relies crucially on steric chaperones in the periplasm. Here, we report the crystal structure of the soluble form of a lipase-specifi...
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Chapter
Protein Secretion Mechanisms in Pseudomonas
The cell envelope of Gram-negative bacteria is composed of two membranes, which are separated by the peptidoglycan-containing periplasm. Whereas the envelope forms an essential barrier aga inst harmful substan...
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Chapter
Biogenesis of Outer Membrane Proteins
The cell envelope of Gram-negative bacteria consists of two membranes, the inner membrane, which is a phospholipid bilayer, and the outer membrane, which is an asymmetrical bilayer with phospholipids and lipop...
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Article
Expression of the pho regulon interferes with induction of the uhpT gene in Escherichia coli K-12
The uptake of hexose 6-phosphates in Escherichia coli is mediated by the transporter UhpT, the synthesis of which is induced by the presence of glucose 6-phosphate (glucose 6-P) in the medium. Since this protein...
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Article
Response of Neisseria meningitidis to iron limitation
In the human body, the concentration of free iron is limiting for bacterial growth, since iron is bound to transport and storage proteins such as transferrin and lactoferrin. When grown under iron starvation, ...
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Chapter and Conference Paper
In vitro assembly of outer membrane protein PhoE of E. coli.
The cell envelope of Gram-negative bacteria, such as Escherichia coli, contains a double membrane. The inner membrane (IM) surrounds the cytoplasm and the outer membrane (OM) protects the cell by forming a barrie...
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Protocol
Chimeric Proteins
Most conventional vaccines consist of killed organisms or purified antigemc components of pathogens. However, this approach of vaccine development has several limitations. Large-scale growth of pathogens may b...
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Article
Effect of mutations in the −10 region of the phoE promoter in Escherichia coli on regulation of gene expression
The phoE promoter region in Escherichia coli contains a −10 region, typical of σ70-dependent promoters and, instead of a normal −35 region, a so-called pho box, to which the transcriptional activator phospho-PhoB...
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Article
Export and assembly of bacterial outer membrane proteins
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Article
Conservation of xcp genes, involved in the two-step protein secretion process, in different Pseudomonas species and other gram-negative bacteria
The two-step protein secretion pathway in Pseudomonas aeruginosa is dependent on the xcp genes. We investigated whether a similar secretion mechanism is present in non-pathogenic Pseudomonas spp. and in other gra...
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Article
Outer membrane protein PhoE as a carrier for the exposure of foreign antigenic determinants at the bacterial cell surface
PhoE protein is an abundant outer membrane protein of theEscherichia coli K-12 outer membrane. This protein can be used as an exposure system to produce foreign antigenic determinants and for their transport to t...
