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    Article

    Dominance and non-complementation among pink-adenineless mutants of Schizophyllum commune involving two discrete genes

    In Schizophyllum commune, adenineless mutations conferring pink color fell into two loosely linked loci, ad-4 and ad-7. The mutations were tested for dominance and complementation in dikaryons, and several of the...

    Yair Parag, Rina Ben-Shaul, Batia Lavie in Molecular and General Genetics MGG (1971)

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    Article

    Heptose-deficient mutants ofEscherichia coli K12 deficient in up to three major outer membrane proteins

    Mutants ofEscherichia coli K12, deficient in up to three major outer membrane proteinsb,c andd have been constructed. Mutants that lack the lipopolysaccharide sugar heptose are deficient in proteinb. All heptose-...

    Wim van Alphen, Ben Lugtenberg, Wim Berendsen in Molecular and General Genetics MGG (1976)

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    Article

    Conjugation deficient E. coli K12 F- mutants with heptose-less lipopolysaccharide

    Two F- mutants deficient in conjugation with F-type donors are isolated and characterized. Phenotypically, these mutants are similar; they have heptose-less lipopolysaccharide and lack some outer membrane protein...

    Louis M. Havekes, Ben J. J. Lugtenberg in Molecular and General Genetics MGG (1976)

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    Article

    Influence of cultural conditions and mutations on the composition of the outer membrane proteins ofEscherichia coli

    VariousEscherichia coli strains differ in the composition of their major outer membrane proteins. However, allE. coli K12 strains tested possess the same major outer membrane proteinsa, b, c andd although quantit...

    Ben Lugtenberg, Roel Peters, Harriet Bernheimer in Molecular and General Genetics MGG (1976)

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    Article

    In vitro transcription of three different ribosomal RNA cistrons of E. coli; heterogeneity of control regions

    Ribosomal RNA synthesis from three different rRNA cistrons of E. coli, located on different phage DNAs was compared and found to have the same characteristics as regards chain length, salt and temperature depende...

    Ben A. Oostra, Albert J. J. van Ooyen, Max Gruber in Molecular and General Genetics MGG (1977)

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    Article

    Genetic map** of the lexC-113 mutation

    A mutation, previously designated lex-113 and suspected to be situated in the lexA locus, has been positioned by transductional studies to a unique site on the chromosome of E. coli B separate from the lexA102 an...

    Ben F. Johnson in Molecular and General Genetics MGG (1977)

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    Article

    Pores in the outer membrane of Escherichia coli K12

    Mutants lacking outer membrane proteins were studied in order to investigate the role of these proteins in the functioning of aqueous pores through the outer membrane. Protein b is involved in the functioning ...

    Wim van Alphen, Nelke van Selm, Ben Lugtenberg in Molecular and General Genetics MGG (1978)

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    Article

    Genetics and biochemistry of the peptidoglycan-associated proteins b and c of Escherichia coli K12

    To collect information on synthesis and regulation of the peptidoglycan-associated pore-forming outer membrane proteins b and c, mutants resistant to phages Mel and TuIa were analyzed. Genetic analysis showed ...

    Cornelis Verhoef, Ben Lugtenberg, Ria van Boxtel in Molecular and General Genetics MGG (1979)

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    Article

    meoA is the structural gene for outer membrane protein c of Escherichia coli K12

    The isolation and characterization of two mutants of Escherichia coli K12 with an altered outer membrane protein c is described. The first mutant, strain CE1151, was isolated as a bacteriophage Mel resistant stra...

    Loek van Alphen, Ben Lugtenberg, Ria van Boxtel in Molecular and General Genetics MGG (1979)

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    Article

    Specific stimulation of ribosomal RNA synthesis in E. coli by a protein factor

    Ribosomal RNA synthesis in a purified system is stimulated by a crude protein fraction prepared from E. coli. The positive effector which is not associated with RNA polymerase, nor is the sigma factor, increases ...

    Ben A. Oostra, AB Geert, Max Gruber in Molecular and General Genetics MGG (1980)

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    Article

    A mutation in the RNA polymerase β′ subunit causing depressed ribosomal RNA synthesis in Escherichia coli

    Macromolecular synthesis in an Escherichia coli mutant with a temperature-sensitive β′ subunit of RNA polymerase was analysed. At the non-permissive temperature ribosomal RNA synthesis is strongly reduced while m...

    Ben A. Oostra, Klaas Kok, Adri J. Van Vliet, AB Geert in Molecular and General Genetics MGG (1981)

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    Article

    Cloning of ompF, the structural gene for an outer membrane pore protein of E. coli K12: Physical localization and homology with the phoE gene

    The ompF gene of Escherichia coli K12, which is the structural gene for an outer membrane pore protein has been cloned into the multicopy plasmid vector pACYC184. Expression of the cloned ompF gene results in ove...

    Jan Tommassen, Peter van der Ley, Arie van der Ende in Molecular and General Genetics MGG (1982)

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    Article

    Phenotypic expression in vivo and transforming activity in vitro: Two related functions of folded bacterial chromosomes

    Nucleoids prepared by gentle lysis of non-complementing diploid cells resulting from Bacillus subtilis protoplast fusion have been used to transform competent cultures of appropriate recipient strains. The yields...

    J. P. Bohin, K. Ben Khalifa, N. Guillen, P. Schaeffer in Molecular and General Genetics MGG (1982)

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    Article

    Suppression of the lexC (ssbA) mutation of Escherichia coli by a mutant of bacteriophage P1

    A new mutant of bacteriophage P1 designated lxc that suppresses the phenotype of lexC and ssbA mutants of Escherichia coli was isolated and characterized. The properties of lexC mutants suppressed by the lxc muta...

    Ben F. Johnson in Molecular and General Genetics MGG (1982)

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    Article

    Analyse génétique de sacB, gène de structure d'une enzyme secrétée, la lévane-saccharase de Bacillus subtilis Marburg

    The structural gene sacB encoding B. subtilis levansucrase, a secreted enzyme, expresses in E. coli. E. coli hosts of the sacB gene are poisoned by sucrose. This property allowed a powerful selection of mutants a...

    Michel Steinmetz, Dominique Le Coq, Hajer Ben Djemia in Molecular and General Genetics MGG (1983)

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    Article

    Cloning of phoM, a gene involved in regulation of the synthesis of phosphate limitation inducible proteins in Escherichia coli K12

    Like the synthesis of alkaline phosphatase, the synthesis of outer membrane PhoE protein is shown to be dependent on the phoM gene product in phoR mutants of E. coli K12. This phoM gene has been cloned into the m...

    Jan Tommassen, Pieter Hiemstra, Piet Overduin in Molecular and General Genetics MGG (1984)

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    Article

    Gene encoding a hybrid OmpF-PhoE pore protein in the outer membrane of Escherichia coli K12

    To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2–73 of the mature PhoE protein is replaced...

    Jan Tommassen, Anthony P. Pugsley, Jaap Korteland in Molecular and General Genetics MGG (1984)

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    Article

    Integration of vector-containing Bacillus subtilis chromosomal DNA by a Campbell-like mechanism

    Using plasmid pHV60, which contains a chloramphenicol resistance (Cmr) gene that is expressed in Bacillus subtilis, a set of transformation-deficient strains of B. subtilis was isolated by insertional mutagenesis...

    Ben Vosman, Jan Kooistra, Jos Olijve, Gerard Venema in Molecular and General Genetics MGG (1986)

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    Article

    Centromeric DNA from Saccharomyces uvarum is functional in Saccharomyces cerevisiae

    Previous comparisons of centromeric DNA sequences in laboratory strains of Saccharomyces cerevisiae have revealed conserved sequences within 120 base pairs (bp) which appear to be essential for centromere functio...

    Joel A. Huberman, R. David Pridmore, Daniel Jäger, Ben Zonneveld in Chromosoma (1986)

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    Article

    Structural plasmid instability in Bacillus subtilis: Effect of direct and inverted repeats

    Using precise excision as a model system, we have quantified the effect of direct repeats, inverted repeats and the size of the spacer between the repeats in the process of deletion formation in Bacillus subtilis

    Ben P. H. Peeters, Jan H. de Boer, Sierd Bron in Molecular and General Genetics MGG (1988)

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