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Article
An improved derivatization method for sensitive determination of fatty acids by high-performance liquid chromatography using 9-(2-hydroxylethyl)-carbazole as derivatization reagent with fluorescence detection
Tagging techniques with reagents used for fluorescent detection for short and long-chain fatty acids using high-performance liquid chromatography are evaluated in terms of the tagging reactions, handing, flexi...
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Article
Determination of biogenic amines by RPHPLC with fluorescent detection after derivatization with 2-(9-carbazole)ethyl chloroformate (CEOC)
The use of 2-(9-carbazole)ethyl chloroformate (CEOC) for pre-column derivatization of biogenic amines (BA) has been tested for the first time. The reagent reacts completely with BA within 3 min at ambient temp...
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Article
Analysis of Five Pharmacologically Active Compounds from Rhodiola for Natural Product Drug Discovery with Capillary Electrophoresis
A rapid capillary electrophoresis method for the separation of five natural pharmacologically active compounds from extracted Rhodiola, namely salidroside, tyrosol, rhodionin, gallic acid and ethyl gallate has be...
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Article
Development of a Sensitive Reagent, 1,2-Benzo-3,4-dihydrocarbazole-9-ethyl-p-toluenesulfonate, for Determination of Bile Acids in Serum by HPLC with Fluorescence Detection, and Identification by Mass Spectrometry with an APCI Source
A pre-column derivatization method with 1,2-benzo-3,4-dihydrocarbazole-9-ethyl-p-toluenesulfonate (BDETS) as labeling reagent followed by high-performance liquid chromatography with fluorescence detection has bee...
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Article
Pre-Column Derivatization of Amines with 1,2-Benzo-3,4-Dihydrocarbazole-9-Isopropyl Chloroformate Followed by LC-Fluorescence and LC-APCI-MS
A pre-column derivatization method for the sensitive determination of amines using the labeling reagent 1,2-benzo-3,4-dihydrocarbazole-9-isopropyl chloroformate (BCIC-Cl) followed by high-performance liquid ch...
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Article
Determination of 30 Free Fatty Acids in Two Famous Tibetan Medicines by HPLC with Fluorescence Detection and Mass Spectrometric Identification
A simple and sensitive high-performance liquid chromatographic (HPLC) method with fluorescence detection and mass spectrometric identification has been developed for analysis of 30 long-chain and short-chain f...
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Article
Mass Spectrometric Identification of Multihydroxy Phenolic Compounds in Tibetan Herbal Medicines
A highly selective and accurate method based on derivatization with dansyl chloride coupled with liquid chromatography–mass spectrometry has been developed for identification of natural pharmacologically activ...
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Article
Determination of Free Fatty Acids in Bryophyte Plants and Soil by HPLC with Fluorescence Detection and Identification by Online MS
A method for the determination of long and short chain free fatty acids (FFAs), using 1-[2-(p-toluenesulfonate)-ethyl]-2-phenylimidazole-[4,5-f]-9,10-phenanthrene (TSPP) as labeling reagent, has been developed. I...
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Article
CE Determination of 2-(9-Carbazole)ethyl Chloroformate-Labeled Oligopeptides
A pre-column derivatization method for sensitive determination of oligopeptides, using the tagging reagent 2-(9-carbazole)ethyl chloroformate (CEOC-Cl) followed by capillary electrophoresis (CE) with diode-arr...
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Article
LC-DAD-ESI-MS Characterization of Carbohydrates Using a New Labeling Reagent
A novel labeling reagent 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) coupling to liquid chromatography with electrospray ionization mass spectrometry for the detection of carbohydrates from the derivatized rape...
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Article
10-Ethyl-acridine-2-sulfonyl Chloride: A New Derivatization Agent for Enhancement of Atmospheric Pressure Chemical Ionization of Estrogens in Urine
The extensive metabolism and treatment of low doses of estrone (E1), estradiol (E2) and estriol (E3) in preclinical animal species necessitates a sensitive analytical method to identify or quantify the estroge...
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Article
Determination and Identification of Primary Aliphatic Amines Using 4-(1H-Phenanthro[9,10-d]Imidazol-2-yl)Benzoic Acid as Novel Pre-Column Labeling Reagent by LC with Fluorescence Detection and Atmospheric Pressure Chemical Ionization Mass Spectroscopy
4-(1H-Phenanthro[9,10-d]imidazol-2-yl)benzoic acid (PIBA), a new amine-reactive fluorescent probe was synthesized and used as a pre-column derivatizing reagent for the determination of aliphatic amines from envir...
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Article
LC–ESI–MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization
Concentrations of 20 free amino aicds (FAAs) in a famous Tibetan medicine Gentiana dahurica was first investigated using 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as the pre-column fluorescence...
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Article
Determination of Free Fatty Acids in Tibet Folk Medicine Potentilla anserina L. Using a New Labeling Reagent by LC with Fluorescence Detection and Identification with Online Atmospheric Chemical Ionization-MS Identification
A sensitive method for the determination of free fatty acids using 1,2-benzo-carbazole-9-ethyl-p-toluenesulfonate (BCETS) as tagging reagent with fluorescence detection has been developed. BCETS could easily and ...
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Article
Comprehensive Comparisons between 1-Phenyl-3-methyl-5-pyrazolones, 1-(4-Methoxyphenyl)-3-methyl-5-pyrazolones and 1-(2-Naphthyl)-3-methyl-5-pyrazolones as Labeling Reagents Used in LC-DAD-ESI-MS-MS Analysis of Neutral Aldoses and Uronic Acids
Comprehensive comparisons between three 1-aryl-3-methyl-5-pyrazolone (AMP) labeling reagents were carried out for the analysis of reductive monosaccharides using reversed phase high performance liquid chromato...
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Article
Determination of Fatty Acids (C1 – C10) from Bryophytes and Pteridophytes
A simple and mild method for the determination of fatty acids (C1 – C10) based on a condensation reaction using 7-aminonaphthalene-1,3-disulfonic acid (ANDSA) as labeling reagent with capillary zone electrophores...
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Article
Determination of Free Fatty Acids from Soil by LC–MS with Fluorescence Detection and Atmospheric Pressure Chemical Ionization Identification
A simple and highly sensitive method for the determination of fatty acids using liquid chromatography with fluorescence detection is described. Fatty acids were derivatized by a new novel pre-column derivatiza...
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Article
LC-Fluorescence Detection Analysis of Amino Acids from Stellera chamaejasme L. Using 2-[2-(Dibenzocarbazole)-ethoxy] Ethyl Chloroformate as Labeling Reagent
A pre-column derivatization method for the sensitive determination of amino acids using the tagging reagent 2-[2-(dibenzocarbazole)-ethoxy] ethyl chloroformate (DBCEC) followed by liquid chromatography with fl...
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Article
LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification
A pre-column derivatization method for the simple, sensitive determination of biogenic amines using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as labeling reagent with fluorescence detection and mass spectro...
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Article
A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)-acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS
A new fluorescence labeling reagent 2-(12-benzo[b]acridin-5(12H)-yl)-acetohydrazide (BAAH) has been designed for fatty acids labeling. Eleven fatty acids containing seven saturated and four unsaturated fatty acid...