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Determination of 30 Free Fatty Acids in Two Famous Tibetan Medicines by HPLC with Fluorescence Detection and Mass Spectrometric Identification

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Abstract

A simple and sensitive high-performance liquid chromatographic (HPLC) method with fluorescence detection and mass spectrometric identification has been developed for analysis of 30 long-chain and short-chain free fatty acids (FFAs). The fatty acids were derivatized to their esters with 1-[2-(p-toluenesulfonate)ethyl]-2-phenylimidazole-[4,5-f]-9,10-phenanthrene (TSPP) in N,N-dimethylformamide (DMF) at 90 °C with anhydrous K2CO3 as catalyst. A mixture of C1–C30 fatty acids was completely separated within 60 min by gradient elution on a reversed-phase C8 column. Qualitative identification of the acids was performed by atmospheric-pressure chemical ionization mass spectrometry (APCI–MS) in positive-ion mode. The fluorescence excitation and emission wavelengths were 260 and 380 nm, respectively. Quantitative determination of the 30 acids in two Tibetan medicines Gentiana straminea and G. dahurica was performed. The results indicated that the medicines contained many FFAs. Linear correlation coefficients for the FFA derivatives were >0.9991. Relative standard deviations (RSDs, n = 6) for the fatty acid derivatives were <3%. Detection limits (at a signal-to-noise ratio of 3:1) were 3.1–38 fmol. When the fatty acid derivatives were determined in the two real samples results were satisfactory and the sensitivity and reproducibility of the method were good.

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References

  1. Chen SH, Chuang YJ (2002) Anal Chim Acta 465:145–155

    Article  CAS  Google Scholar 

  2. Seppänen-Laakso T, Laakso I, Hiltunen R (2002) Anal Chim Acta 465:39–62

    Article  Google Scholar 

  3. Señoráns FJ, Ibañez E (2002) Anal Chim Acta 465:131–144

    Article  Google Scholar 

  4. You JM, Zhang WB, Zhang YK (2001) Anal Chim Acta 436:163–172

    Article  CAS  Google Scholar 

  5. Chen SH, Chen KW, Lien HM (1999) J Chromatogr A 849:357–369

    Article  CAS  Google Scholar 

  6. Li Y, Lander R, Manger W, Lee A (2004) J Chromatogr B 804:353–358

    Article  CAS  Google Scholar 

  7. Bravo B, Chávez G, Piña N, Ysambertt F, Márquez N, Cáceres A (2004) Talanta 64:1329–1334

    Article  CAS  Google Scholar 

  8. Peris-Vicente J, Gimeno-Adelantado JV, Doménech-Carbó MT, Mateo-Castro R, Bosch-Reig F (2005) J Chromatogr A 1076:44–50

    CAS  Google Scholar 

  9. Czauderna M, Kowalczyk J (2001) J Chromatogr B 760:165–178

    Article  CAS  Google Scholar 

  10. Rosenfeld JM (2001) Anal Chim Acta 465:93–100

    Article  Google Scholar 

  11. Takadate A, Masuda T, Murata C, Haratake C, Isobe A, Irikara M, Goya S (1992) Anal Sci 8:695–698

    CAS  Google Scholar 

  12. Yoshida T, Uetake A, Yamaguchi H, Nimura N, Kinoshita T (1988) Anal Biochem 173:70–74

    Article  CAS  Google Scholar 

  13. Lu CY, Wu HL, Chen SH, Kou HS (2000) Chromatographia 51:315–321

    Article  CAS  Google Scholar 

  14. Ho TN, Liu SW (eds) (2001) A worldwide monograph of Gentiana. Science Press, Bei**g, p 176

  15. Yang YC (ed) (1991) Tibetan medicines. **ning, p 9

  16. Ji LJ, Sun HF, Ding JY, Fan SF, Hu BL (1992) Acta Biol Plateau Sin 11:113–118

    Google Scholar 

  17. Beith M (1995) Healing threads: traditional medicines of the highlands and islands. Edinburgh, Scotland, pp 1–304

    Google Scholar 

  18. Kumarasamy Y, Nahar L, Sarker SD (2003) Fitoterapia 74:151–154

    Article  CAS  Google Scholar 

  19. Guo YJ, Lu YR (1983) Chin J Pharm Anal 3:268–271

    CAS  Google Scholar 

  20. Luo JP, Lou CZ (1985) Chin Tradit Herb Drugs 5:7–10

    CAS  Google Scholar 

  21. Ji LJ, Liao ZX, Sun HF (2002) Acta Biol Plateau Sin 15:243–250

    Google Scholar 

  22. Ji LJ, Ma YH, Chen GC, Zhou GY, Dai DH, Sun HF (2004) Acta Bot Boreal Occident Sin 24:292–295

    CAS  Google Scholar 

  23. Duan YW, He YP, Liu JQ (2005) Acta Oecol 27:225–232

    Article  Google Scholar 

  24. He YP, Liu JQ (2004) Acta Ecol Sin 24:215–220

    Google Scholar 

  25. Shi SB, Han F, Li HY (2001) Acta Phytophysiol Sin 27:123–128

    Google Scholar 

  26. Shi SB, Zhu WY, Li HM, Zhou DW, Han F, Zhao XQ, Tang YH (2004) Environ Exp Bot 51:75–83

    Article  CAS  Google Scholar 

  27. Sun J, Lin CQ, Wang HL, Suo YR, Chen GC, Li TC (2004) Guangdong Weiliang Yuansu Kexue 11:37–39

    CAS  Google Scholar 

  28. Wang HL, Lin CQ, Sun J, Chen GC, Suo YR (2004) Guangdong Weiliang Yuansu Kexue 11:45–47

    Google Scholar 

  29. Zhao XE, Sun J, Suo YR, Liu SJ, Chen GC, Sun XJ, You JM (2006) J Instrum Anal 5:1–6

    Google Scholar 

  30. Zhao XE, Suo YR, Ding CX, Zhu F, Sun J, Zhao WC, Sun XJ, You JM (2006) Chin J Chromatogr 24:456–461

    CAS  Google Scholar 

  31. You J, Shi Y, Ming Y, Yu Z, Yi Y, Liu J (2004) Chromatographia 60:527–535

    Article  CAS  Google Scholar 

  32. Kishikawa N, Wada M, Ohba Y, Nakashima K, Kuroda N (2004) J Chromatogr A 1057:83–88

    Article  CAS  Google Scholar 

  33. Munzuroglu O, Karatas F, Geckil H (2003) Food Chem 83:205–212

    Article  CAS  Google Scholar 

  34. Echeverrigaray S, Fracaro F, Santos ACA, Paroul N, Wasum R, Serafini LA (2003) Biochem Syst Ecol 31:467–475

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was funded by grants from the National Mid-West Fund of China (No. 2001BA901A47) and the Knowledge Innovation Programs of the Chinese Academy of Sciences (No. CXLY-2002-08).

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Authors and Affiliations

  1. Research Center of National Tibetan Medicine, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 59 **guan Street, **ning, 810008, People’s Republic of China

    **g Sun, Guichen Chen, **anen Zhao, Wenhua Xu, Guoying Zhou & Youji Han

  2. Graduate School of the Chinese Academy of Sciences, Bei**g, 100049, People’s Republic of China

    **g Sun, **anen Zhao, Wenhua Xu, Guoying Zhou & Youji Han

  3. College of Chemistry Science, Qufu Normal University, Qufu, 273165, People’s Republic of China

    **mao You

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  1. **g Sun
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  2. Guichen Chen
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  3. **anen Zhao
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  4. Wenhua Xu
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  5. Guoying Zhou
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Correspondence to Guichen Chen.

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Sun, J., Chen, G., Zhao, X. et al. Determination of 30 Free Fatty Acids in Two Famous Tibetan Medicines by HPLC with Fluorescence Detection and Mass Spectrometric Identification. Chroma 65, 469–476 (2007). https://doi.org/10.1365/s10337-007-0171-4

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  • DOI: https://doi.org/10.1365/s10337-007-0171-4

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