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CE Determination of 2-(9-Carbazole)ethyl Chloroformate-Labeled Oligopeptides

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Abstract

A pre-column derivatization method for sensitive determination of oligopeptides, using the tagging reagent 2-(9-carbazole)ethyl chloroformate (CEOC-Cl) followed by capillary electrophoresis (CE) with diode-array detection, has been developed. Maximum yield close to 100% were observed when a three to fourfold molar excess of reagent was used at pH 9.0–10.0. Excess reagent was extracted with n-hexane–ethyl acetate 9:1–10:1 (v/v); this enabled direct analysis using CE with no significant disturbance from the major fluorescent reagent degradation by-products. The effects on the results of buffer pH and of SDS and organic modifier concentrations were examined. Good baseline resolution in the separation of five CEOC-peptides was achieved with a 48.5-cm total length (effective length 40 cm) 50-μm inner diameter capillary column.

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Acknowledgments

The National Science Foundation under grant #20075016 and the “Wester Light” program of talent cultivation of The Chinese Academy of Sciences (CAS) supported this research.

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Authors and Affiliations

  1. Northwest Plateau Institute of Biology, Chinese Academy of Sciences, **ning Qinghai, 810001, People’s Republic of China

    Chenxu Ding, **anen Zhao, Wenchen Zhao, Yulin Li, Honglun Wang, Yourui Suo & **mao You

  2. College of Chemistry Science, Qufu Normal University, Qufu Shandong, 273165, People’s Republic of China

    Xuejun Sun, Wenchen Zhao & **mao You

  3. Department of Pharmacy, Qinghai Nationalities Institute, **ning Qinghai, 810007, People’s Republic of China

    Chenxu Ding

  4. Graduate School of the Chinese Academy of Sciences, Bei**g, 100039, People’s Republic of China

    Chenxu Ding & **anen Zhao

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  1. Chenxu Ding
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  2. Xuejun Sun
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Correspondence to **mao You.

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Ding, C., Sun, X., Zhao, X. et al. CE Determination of 2-(9-Carbazole)ethyl Chloroformate-Labeled Oligopeptides. Chroma 67, 245–251 (2008). https://doi.org/10.1365/s10337-007-0475-4

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