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Protocol
Using Open Chromatin Enrichment and Network Hi-C (OCEAN-C) to Identify Open Chromatin Interactions
The open chromatin enrichment and network Hi-C (OCEAN-C) was developed not only for identifying large-scale chromatin structures, including topologically associated domains (TADs) and A/B compartments, but als...
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Protocol
Quantification of d- and l-2-Hydroxyglutarate in Drosophila melanogaster Tissue Samples Using Gas Chromatography-Mass Spectrometry
The fruit fly Drosophila melanogaster has emerged as an ideal system in which to study 2-hydroxyglutarate (2HG) metabolism. Unlike many mammalian tissues and cell lines, which primarily accumulate d- or l-2HG as ...
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Protocol
Haplotype Inference
Haplotypes, as they specify linkage patterns between individual nucleotide variants, confer critical information for understanding the genetics of human diseases. However, haplotype information is not directly...
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Protocol
Exploiting Natural Diversity of AAV for the Design of Vectors with Novel Properties
Twelve AAV serotypes have been described so far in human and nonhuman primate (NHP) populations while surprisingly high diversity of AAV sequences is detected in tissue biopsies. The analysis of these novel AA...
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Protocol
Gene Therapy in Skeletal Muscle Mediated by Adeno-Associated Virus Vectors
Adeno-associated virus (AAV) is the most promising gene delivery vehicle for muscle-directed gene therapy. AAV’s natural tropism to muscle cells, long-term persistent transgene expression, multiple serotypes, ...
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Protocol
Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential
Sequences with the potential to form intramolecular G-quadruplexes (G4-structures) are found in highly nonrandom distributions in the genomes of diverse organisms. These sequences are associated with nucleic a...
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Protocol
An Introduction to Mitochondrial Informatics
In this chapter, we review the public resources available for human mitochondrial DNA and protein related bioinformatics, with a special focus on mitochondrial single nucleotide polymorphisms (mtSNPs). We also...
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Protocol
In Vitro and In Vivo Gene Silencing by TransKingdom RNAi (tkRNAi)
RNA interference (RNAi) is a potent and specific mechanism for eliminating the mRNA of specific genes. This gene silencing mechanism occurs naturally and is highly conserved from plants to human cells, holding...
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Protocol
Application of ESTs in Microarray Analysis
Microarray analyses provide information on the relative expression levels of large numbers of gene products (transcripts). As such they have been widely used to examine differences in gene expression across a ...
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Protocol
Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in Vivo
The mammalian two-hybrid system is a very powerful tool to investigate protein-protein interactions in terms of functional domains and identify potential binding ligands and partners of a protein. Compared wit...
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Protocol
Application of siRNA Against SARS in the Rhesus Macaque Model
Containment of the SARS coronavirus (SCV) outbreak was accompanied by the rapid characterization of this new pathogen's genome sequence in 2003, encouraging the development of anti-SCV therapeutics using short...
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Protocol
High-Throughput Genoty** of Single Nucleotide Polymorphisms with High Sensitivity
The ability to analyze a large number of genetic markers consisting of single nucleotide polymorphisms (SNPs) may bring about significant advance in understanding human biology. Recent development of several h...
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Protocol
Protein Chip for Detection of DNA Mutations
A large number of human genetic diseases, bacterial drug resistances, and single-nucleotide polymorphisms are caused by gene mutations. Rapid and high-throughput mutation detection methods are urgently demande...
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Protocol
Designing PCR Primer for DNA Methylation Map**
DNA methylation is an epigenetic mechanism of gene regulation, and aberrant methylation has been associated with various types of diseases, especially cancers. Detection of DNA methylation has thus become an i...
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Protocol
In Silico Prediction of Peptide-MHC Binding Affinity Using SVRMHC
The binding between peptide epitopes and major histocompatibility complex (MHC) proteins is a major event in the cellular immune response. Accurate prediction of the binding between short peptides and class I ...
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Protocol
Nanotechnology
Microarrays are important tools for high-throughput analysis of biomolecules. The use of microarrays for parallel screening of nucleic acid and protein profiles has become an industry standard. A few limitatio...
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Protocol
Signal Processing and the Design of Microarray Time-Series Experiments
Recent findings of a genome-wide oscillation involving the transcriptome of the budding yeast Saccharomyces cerevisiae suggest that the most promising path to an understanding of the cell as a dynamic system will...
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Protocol
Uses of Telomerase Peptides in Anti-Tumor Immune Therapy
Human telomerase reverse transcriptase (hTERT) represents a universal tumor-associated antigen to activate specific immune response in cancer immune therapy. Peptides derived from hTERT are presented by major ...
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Protocol
Knockdown of Telomerase RNA Using Hammerhead Ribozymes and RNA Interference
More than 85% of human cancers and over 70% of immortalized human cell lines have highly elevated telomerase activity. In contrast, telomerase activity is down-regulated in most human adult somatic cells, exce...
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Protocol
Inhibition of Telomerase by Targeting MAP Kinase Signaling
Constitutive activation of the mitogen-activated protein (MAP) kinase signaling pathway by oncogenic stimulation is widespread in human cancers. With the recently demonstrated links between MAP kinase, histone...