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  1. Article

    Open Access

    Structural analysis of the regulatory mechanism of MarR protein Rv2887 in M. tuberculosis

    MarR family proteins are transcriptional regulators that control expression of bacterial proteins involved in metabolism, virulence, stress responses and multi-drug resistance, mainly via ligand-mediated atten...

    Yun-Rong Gao, De-Feng Li, Joy Fleming, Ya-Feng Zhou, Ying Liu… in Scientific Reports (2017)

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  2. Article

    Open Access

    Cyclic di-GMP regulates Mycobacterium tuberculosis resistance to ethionamide

    Tuberculosis is still on the top of infectious diseases list on both mobility and mortality, especially due to drug-resistance of Mycobacterium tuberculosis (M.tb). Ethionamide (ETH) is one of effective second li...

    Hai-Nan Zhang, Zhao-Wei Xu, He-Wei Jiang, Fan-Lin Wu, **ang He… in Scientific Reports (2017)

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  3. No Access

    Article

    A Novel T-type Overhangs Improve the Enzyme-Free Cloning of PCR Products

    PCR product cloning is the foundational technology for almost all fields in the life sciences. Numerous innovative methods have been designed during the past few decades. Enzyme-free cloning is the only one th...

    Ren-Bin Yang, Li-Jun Bi, **an-En Zhang in Molecular Biotechnology (2013)

  4. Article

    Crystal structure of L,D-transpeptidase LdtMt2 in complex with meropenem reveals the mechanism of carbapenem against Mycobacterium tuberculosis

    Wen-Juan Li, De-Feng Li, Yong-Lin Hu, **an-En Zhang, Li-Jun Bi… in Cell Research (2013)

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  5. Article

    A simplified method for reconstituting active E. coli DNA polymerase III

    Genome duplication in E. coli is carried out by DNA polymerase III, an enzyme complex consisting of ten subunits. Investigations of the biochemical and structural properties of DNA polymerase III require the expr...

    Shi-Qiang Lin, Li-Jun Bi, **an-En Zhang in Protein & Cell (2011)

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  6. No Access

    Article

    Application of methyl parathion hydrolase (MPH) as a labeling enzyme

    Methyl parathion hydrolase (MPH) is an enzyme that catalyzes the degradation of methyl parathion, generating a yellow product with specific absorption at 405 nm. The application of MPH as a new labeling enzyme...

    Wei Yang, Ya-Feng Zhou, He-** Dai, Li-Jun Bi… in Analytical and Bioanalytical Chemistry (2008)

  7. No Access

    Protocol

    Protein Chip for Detection of DNA Mutations

    A large number of human genetic diseases, bacterial drug resistances, and single-nucleotide polymorphisms are caused by gene mutations. Rapid and high-throughput mutation detection methods are urgently demande...

    **an-En Zhang, Li-Jun Bi in Microarrays (2007)

  8. No Access

    Article

    Reversible immobilization of proteins with streptavidin affinity tags on a surface plasmon resonance biosensor chip

    Dissociation of biotin from streptavidin is very difficult due to their high binding affinity. The re-use of streptavidin-modified surfaces is therefore almost impossible, making devices containing them (e.g. ...

    Yong-** Li, Li-Jun Bi, **an-En Zhang… in Analytical and Bioanalytical Chemistry (2006)