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    Protocol

    Repurposing Photosensitizer Proteins Through Genetic Code Expansion to Facilitate Photo-Biocatalysis

    Artificial photoenzymes with noncanonical photo-redox cofactors have paved the way for enzyme rational design and the creation of new-to-nature biocatalysts. Genetically encoded photo-redox cofactors endow pho...

    Jiangyun Wang, Yan **a, Xuzhen Guo in Genetically Incorporated Non-Canonical Amino Acids (2023)

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    Protocol

    Human CD4+CD25highCD127low/neg Regulatory T Cells

    CD4+CD25highCD127low/neg regulatory T cells (Tregs) play a critical role in the maintenance of peripheral tolerance and in controlling the development of autoimmune diseases. A combination of surface and intracel...

    Haibin Su, Maria Serena Longhi, Pengyun Wang, Diego Vergani… in Human Cell Culture Protocols (2012)