124 Result(s)
within
Teruo Okano
English
-
Article
Open AccessHepatocyte Transplantation: Cell Sheet Technology for Liver Cell Transplantation
We will review the recent developments of cell sheet technology as a feasible tissue engineering approach. Specifically, we will focus on the technological advancement for engineering functional liver tissue u...
-
Article
Open AccessEnhanced Therapeutic Effects of Human iPS Cell Derived-Cardiomyocyte by Combined Cell-Sheets with Omental Flap Technique in Porcine Ischemic Cardiomyopathy Model
Transplant of human induced pluripotent stem cell derived cardiomyocytes (hiPS-CMs) cell-sheet is a promising approach for treating ischemic cardiomyopathy (ICM). However, poor blood supply to the transplanted...
-
Article
Open AccessBio-implant as a novel restoration for tooth loss
A dental implant is used to replace a missing tooth. Fixing the implant in its natural position requires the engineering of a substantial amount of conformal bone growth inside the implant socket, osseointegra...
-
Article
Open AccessMiddle ear mucosal regeneration by tissue-engineered cell sheet transplantation
The recurrence of cholesteatoma after surgical treatment often occurs as a result of poor mucosal regeneration in the middle ear cavity and mastoid cavity and changes, such as granulation tissue formation, whi...
-
Article
Open AccessThicker three-dimensional tissue from a “symbiotic recycling system” combining mammalian cells and algae
In this paper, we report an in vitro co-culture system that combines mammalian cells and algae, Chlorococcum littorale, to create a three-dimensional (3-D) tissue. While the C2C12 mouse myoblasts and rat cardiac ...
-
Article
Design of Tetra-arm PEG-crosslinked Thermoresponsive Hydrogel for 3D Cell Culture
We synthesized poly(N-isopropylacrylamide) gel containing cell-adhesive peptides. RGDS pendants, and crosslinked by a hydrophilic polymer. Since gelation occurs by simply mixing the polymers, cells can be simulta...
-
Article
Retraction Note: Reproducible subcutaneous transplantation of cell sheets into recipient mice
Nat. Protoc. 6, 1053–1059 (2011); published online 30 June 2011; retracted 13 January 2016 Masayuki Yamato, Satoshi Tsuneda and Teruo Okano would like to retract this protocol after concerns were raised by the...
-
Article
Open AccessTRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues
The development of a suitable strategy for eliminating remaining undifferentiated cells is indispensable for the use of human-induced pluripotent stem (iPS) cell-derived cells in regenerative medicine. Here, w...
-
Chapter
Cell Sheet Tissue Engineering for Heart Failure
In recent years, regenerative medicine using cells for treating tissue defects has been in the spotlight as a new treatment for severe heart failure. Direct injection of bone marrow-derived cells and isolated ...
-
Article
Cell Sheets for Periodontal Tissue Engineering
Cell Sheet Technology was first utilized in a clinical trial for corneal reconstruction in 2003; since then, several clinical trials have been performed not only in Japan but also in Europe. The key technology...
-
Article
Open AccessHuman mesenchymal stem cell-engineered hepatic cell sheets accelerate liver regeneration in mice
Mesenchymal stem cells (MSCs) are an attractive cell source for cell therapy. Based on our hypothesis that suppression of Wnt/β-catenin signal enhances hepatic differentiation of human MSCs, we developed human...
-
Article
Use of a microchamber for analysis of thermal variation of the gliding phenomenon of single Navicula pavillardii cells
In this study, we used a microchamber to observe and analyze the gliding phenomenon of Navicula pavillardii diatom cells at different temperatures. The temperature of the culture medium was varied from 17.0 to 3...
-
Article
Measurement of the dynamic behavior of thin poly(N-isopropylacrylamide) hydrogels and their phase transition temperatures measured using reflectometric interference spectroscopy
Temperature-responsive cell culture surfaces prepared by modifying tissue-culture polystyrene with nanoscale poly(N-isopropylacrylamide) (PIPAAm) hydrogels are widely used as intelligent surfaces for the fabricat...
-
Article
Control of Cell Adhesion and Detachment on Temperature-Responsive Block Copolymer Langmuir Films
This study used Langmuir-Schaefer (LS) method to produce thermo-responsive poly(N-isopropylacrylamide) (PIPAAm) modified surface. Block copolymer composed of polystyrene (PSt) and PIPAAm was synthesized by RAFT p...
-
Article
Biofunctional Thermo-Responsive Polymeric Surface with Micropatterns for Label Free Cell Separation
Thready stripe-patterned thermo-responsive surfaces were prepared and their surface properties were characterized. Prepared 3 μm wide stripe-patterned surfaces were evaluated by observing the adhesions and det...
-
Article
Open AccessHuman iPS cell-engineered cardiac tissue sheets with cardiomyocytes and vascular cells for cardiac regeneration
To realize cardiac regeneration using human induced pluripotent stem cells (hiPSCs), strategies for cell preparation, tissue engineering and transplantation must be explored. Here we report a new protocol for ...
-
Article
Nov/CCN3 regulates long-term repopulating activity of murine hematopoietic stem cells via integrin αvβ3
Throughout life, hematopoietic stem cells (HSCs) sustain the blood cell supply through their capacities for self-renewal and multilineage differentiation. These processes are regulated within a specialized mic...
-
Protocol
Cell Sheet Technology for Cardiac Tissue Engineering
In this chapter, we describe the methods for the fabrication and transfer/transplantation of 3D tissues by using cell sheet technology for cardiac tissue regeneration. A temperature-responsive culture surface ...
-
Article
Latest status of the clinical and industrial applications of cell sheet engineering and regenerative medicine
Cell sheet engineering, which allows tissue engineering to be realized without the use of biodegradable scaffolds as an original approach, using a temperature-responsive intelligent surface, has been applied i...
-
Article
Open AccessRegenerative medicine of cornea by cell sheet engineering using temperature-responsive culture surfaces
Recently, regenerative medicine has been focused on as next-generation definitive therapies for several diseases or injuries for which there are currently no effective treatments. These therapies have been rap...
