SpringerLink
Log in

Use of a microchamber for analysis of thermal variation of the gliding phenomenon of single Navicula pavillardii cells

  • Original Paper
  • Published:
European Biophysics Journal Aims and scope Submit manuscript

Abstract

In this study, we used a microchamber to observe and analyze the gliding phenomenon of Navicula pavillardii diatom cells at different temperatures. The temperature of the culture medium was varied from 17.0 to 30.0 °C to examine the effect of temperature on diatom movement. Movement of each cell at different temperatures was monitored by use of an inverted optical microscope and continuously recorded as video data, from which the velocities of each cell were calculated, by using dedicated software to perform two-dimensional trajectory analysis. The velocities of the same cell at different temperatures were thereby successfully compared. The results showed that the change in cell velocity was insignificant when the temperature was increased from 17.0 to 25.0 °C. When the temperature was increased from 17.0 to 27.5 °C, non-uniformly disrupted cell movement was observed. When the temperature was further increased to 30.0 °C, cell movement was clearly inhibited. By use of single-cell analysis, the effects of the temperature increases on diatom movement were successfully evaluated. Finally, we characterized the experimental data by performing t tests to evaluate the effects of variations of the movement of individual cells on the data analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Apoya-Horton MD, Liang Y, Underwood GJC, Gretz MR (2006) Movement modalities and responses to environmental changes of the mudflat diatom Cylindrotheca closterium (Bacillariophyceae). J Phycol 42:379–390. doi:10.1111/j.1529-8817.2006.00194.x

    Article  CAS  Google Scholar 

  • Armbrust EV (2009) The life of diatoms in the world’s oceans. Nature 459:185–192. doi:10.1038/nature08057

    Article  CAS  PubMed  Google Scholar 

  • Bowler C, Vardi A, Allen AE (2010) Oceanographic and biogeochemical insights from diatom genomes. Ann Rev Mar Sci 2:333–365. doi:10.1146/annurev-marine-120308-081051

    Article  PubMed  Google Scholar 

  • Cartaxana P, Serôdio J (2008) Inhibiting diatom motility: a new tool for the study of the photophysiology of intertidal microphytobenthic biofilms. Limnol Oceanogr Methods 6:466–476. doi:10.4319/lom.2008.6.466

    Article  CAS  Google Scholar 

  • Clarkson N, Davies MS, Dixey R (1999) Diatom motility and low frequency electromagnetic fields: a new technique in the search for independent replication of results. Bioelectromagnetics 20:94–100. doi:10.1002/(SICI)1521-186X(1999)20:2<94::AID-BEM3>3.0.CO;2-Q

    Article  CAS  PubMed  Google Scholar 

  • Cohn S (2001) Photo-stimulated effects on diatom motility. In: Hader DP, Lebert M (eds) Photomovement. Elsevier, Amsterdam, pp 375–401

    Chapter  Google Scholar 

  • Cohn SA, Farrell JF, Munro JD, Ragland RL, Weitzell RE Jr, Wibisono BL (2003) The effect of temperature and mixed species composition on diatom motility and adhesion. Diatom Res 18:225–243

    Article  Google Scholar 

  • Cohn SA, Bahena M, Davis JT, Ragland RL, Rauschenberg CD, Smith BJ (2004) Characterization of the diatom photophobic response to high irradiance. Diatom Res 19:167–179

    Article  Google Scholar 

  • Consalvey M, Paterson DM, Underwood GJC (2004) The ups and downs of life in a benthic biofilm: migration of benthic diatoms. Diatom Res 19:181–202

    Article  Google Scholar 

  • Cooksey B, Cooksey KE (1980) Calcium is necessary for motility in the diatom Amphora coffeaeformis. Plant Physiol 65:129–131. doi:10.1104/pp.65.1.129

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Drum RW, Hopkins JT (1966) Diatom locomotion: an explanation. Protoplasma 62:1–33. doi:10.1007/BF01254629

    Article  Google Scholar 

  • Edgar LA (1979) Diatom locomotion: computer assisted analysis of cine film. Br Phycol J 14:83–101. doi:10.1080/00071617900650111

    Article  Google Scholar 

  • Edgar LA (1982) Diatom locomotion: a consideration of movement in a highly viscous situation. Br Phycol J 17:243–251. doi:10.1080/00071618200650261

    Article  Google Scholar 

  • Edgar LA (1983) Mucilage secretions of moving diatoms. Protoplasma 118:44–48. doi:10.1007/BF01284745

    Article  Google Scholar 

  • Edgar LA, Pickett-Heaps JD (1982) Ultrastructural localization of polysaccharides in the motile diatom Navicula cuspidate. Protoplasma 113:10–22. doi:10.1007/BF01283035

    Article  CAS  Google Scholar 

  • Edgar LA, Pickett-Heaps JD (1983) The mechanism of diatom locomotion. I. An ultrastructural study of the motility apparatus. Proc R Soc Lond B218:331–343. doi:10.1098/rspb.1983.0042

    Article  Google Scholar 

  • Edgar LA, Pickett-Heaps JD (1984) Diatom locomotion. Prog Phycol Res 3:47–88

    Article  Google Scholar 

  • Edgar LA, Zavortink M (1983) The mechanism of diatom locomotion. II: identification of Actin. Proc R Soc Lond B218:345–348. doi:10.1098/rspb.1983.0043

    Article  Google Scholar 

  • Falkowski PG, Raven JA (1997) Aquatic photosynthesis. Blackwell Science, Malden

    Google Scholar 

  • Gordon R, Drum RW (1970) A capillarity mechanism for diatom gliding locomotion. Proc Natl Acad Sci USA 67:338–344. doi:10.1073/pnas.67.1.338

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Harper MA (1977) The biology of diatoms. In: Werner D (Ed.), Blackwell Scientific Publications, Oxford, pp 224–249. doi:10.1016/0014-4827(72)90416-8

  • Iwasa K, Shimizu A (1972) Motility of the diatom, Phaeodactylum tricornutum. Exp Cell Res 74:552–558. doi:10.1016/0014-4827(72)90416-8

    Article  CAS  PubMed  Google Scholar 

  • Lewin JC (1958) The taxonomic position of Phaeodactylum tricornutum. J Gen Microbiol 18:427–432. doi:10.1099/00221287-18-2-427

    Article  CAS  PubMed  Google Scholar 

  • Moroz AL, Ehrman JM, Clair TA, Gordon RJ, Kaczmarska I (1999) The impact of ultraviolet-B radiation on the motility of the freshwater epipelic diatom Nitzschia lineariz. Global Change Biol 5:191–199. doi:10.1046/j.1365-2486.1999.00225.x

    Article  Google Scholar 

  • Murase A, Kubota Y, Hirayama S, Kumashiro Y, Okano T, Mayama S, Umemura K (2011) Two-dimensional trajectory analysis of the diatom Navicula sp. using a micro chamber. J Microbiol Methods 87(3):316–319. doi:10.1016/j.mimet.2011.09.006

    Article  PubMed  Google Scholar 

  • Murase A, Kubota Y, Hori S, Hirayama S, Mayama S (2012) Importance of observation interval in two-dimensional video analysis of individual diatom cells. Eur Biophys J 41(6):545–550. doi:10.1007/s00249-012-0810-z

    Article  PubMed  Google Scholar 

  • Nelson DM, Treguer P, Brzezinski MA, Leynaert A, Queguiner B (1995) Production and dissolution of biogenic silica in the ocean: revised global estimates comparison with regional data and relationship to biogenic sedimentation. Global Biogeochem Cycles 9:359–372. doi:10.1029/95GB01070

    Article  CAS  Google Scholar 

  • Perkins RG, Lavaud J, Serôdio J, Mouget JL, Cartaxana P, Rosa P, Barille L, Brotas V, Jesus BM (2010a) Vertical cell movement is a primary response of intertidal benthic biofilms to increasing light dose. Mar Ecol Progr Ser 416:93–103. doi:10.3354/meps08787

    Article  CAS  Google Scholar 

  • Perkins RG, Kromkamp JC, Serôdio J, Lavaud J, Jesus B, Mouget JL, Lefebvre S, Forster RM (2010) The application of variable chlorophyll fluorescence to microphytobenthic biofilms. In: Suggett DJ et al (eds) Chlorophyll a fluorescence in aquatic sciences: methods and applications, developments in applied phycology 4. doi:10.1007/978-90-481-9268-7_12

  • Pickett-Heaps JD, Hill DRA, Blaze KL (1991) Active gliding motility in an araphid marine diatom, Ardissonea (formerly synedra) crystalline. J Phycol 27:718–725. doi:10.1111/j.0022-3646.1991.00718.x

    Article  Google Scholar 

  • Poulsen NC, Spector I, Spurck TP, Schultz TF, Wetherbee R (1999) Diatom gliding is the result of an actin–myosin motility system. Cell Motil Cytoskelet 44:23–33. doi:10.1002/(SICI)1097-0169(199909)44:1::23:AID-CM2[3.0.CO;2-D

  • Umemura K, Haneda T, Tanabe M, Suzuki A, Kumashiro Y, Itoga K, Okano T, Mayama S (2013) Semi-circular micro grooves to observe active movements of individual Navicula pavillardii cells. J Microbiol Meth 92:349–354. doi:10.1016/j.mimet.2013.01.006

    Article  Google Scholar 

  • Underwood GJC, Kromkamp J (1999) Primary production by phytoplankton and microphytobenthos in estuaries. Adv Ecol Res 29:93–153. doi:10.1016/S0065-2504(08)60192-0

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by RIST TUS Research Center for Green and Safety Sciences.

Conflict of interest

There is no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Physics, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 162-8601, Japan

    Kazuo Umemura, Toru Miyabayashi, Hisao Taira & Akira Suzuki

  2. Institute of Advanced Biomedical Engineering and Science (TWins), Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan

    Yoshikazu Kumashiro & Teruo Okano

  3. Faculty of Education, Tokyo Gakugei University, 4-1-1 Nukui-kita-machi, Koganei, Tokyo, 184-8511, Japan

    Shigeki Mayama

Authors
  1. Kazuo Umemura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Toru Miyabayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hisao Taira
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Akira Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yoshikazu Kumashiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Teruo Okano
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shigeki Mayama
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kazuo Umemura.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Umemura, K., Miyabayashi, T., Taira, H. et al. Use of a microchamber for analysis of thermal variation of the gliding phenomenon of single Navicula pavillardii cells. Eur Biophys J 44, 113–119 (2015). https://doi.org/10.1007/s00249-015-1006-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00249-015-1006-0

Keywords

Search

Navigation