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Ion-pair Reversed-phase×Low-pH Reversed-phase Two-dimensional Liquid Chromatography for In-depth Proteomic Profiling
H igh-resolution liquid chromatography separation is essential to in-depth proteomic profiling of complex biological samples. Herein, we established...
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Determination of Polar Veterinary Drugs and Melamine in Feeds: Method Using Ion-Pair Reversed-Phase or Hydrophilic Interaction Liquid Chromatography Coupled to Tandem Mass Spectrometry
This protocol specifies a LC-ESI-MS/MS method for the quantification of the polar veterinary drugs spectinomycin, zilpaterol, halquinol (a...
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Ion-pair solvent-based liquid–liquid microextraction and spectrophotometric determination of E102, E110, E124, E129 and E133 in confectioneries and beverages
In the present study, a new ion-pair solvent including tetra-n-butyl-ammonium iodide and 1-pentanol was prepared for the first time and it was used...
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A high-resolution measurement of nucleotide sugars by using ion-pair reverse chromatography and tandem columns
N-Linked glycosylation is a cellular process transferring sugars from glycosyl donors to proteins or lipids. Biopharmaceutical products widely...
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Gas Chromatography and Mass Spectrometry: The Technique
Combining the techniques of gas chromatography and mass spectrometry provides conjunction with a confirmatory character, which has a great power of...
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Origins and Formation of Ion Chromatography
AbstractThe origins and ways of formation of ion chromatography are shown. Little-known facts and publications are reflected, and a significant...
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Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors
Human immunodeficiency virus (HIV)-1 reverse transcriptase (HIV-1 RT) is responsible for the transcription of viral RNA genomes into DNA genomes and...
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2D Chromatography and 2D Spectroscopy in Analytical Chemistry: an Overview
AbstractThe development of rapid chromatographic procedures is of utmost relevance to analytical laboratories since the modern pharmaceutical...
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Efficient separation of uranium and lanthanides based on high-performance ion exchange chromatography
This study presents an efficient method for the sequential separation of lanthanides and uranium using a high-performance ion exchange...
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Recent Trends in Suppressor Techniques for Ion Chromatography
This review summarizes the progress in suppressor over the period from 1981 to 2022, with an emphasis on the last ten years. Although quite well...
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Adsorbents with a Covalently Bonded Polymer Layer for Hydrophilic Interaction Liquid Chromatography
AbstractStationary phases based on silica modified with polyvinylpyridine and polyethyleneimine of different structures using 1,4-butanediol...
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Deep eutectic solvent self-assembled reverse nanomicelles for transdermal delivery of sparingly soluble drugs
BackgroundTransdermal delivery of sparingly soluble drugs is challenging due to their low solubility and poor permeability. Deep eutectic solvent...
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Liquid chromatography–high-resolution tandem mass spectrometry of anatoxins, including new conjugates and reduction products
Anatoxins (ATXs) are a potent class of cyanobacterial neurotoxins for which only a handful of structural analogues have been well characterized....
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Supercritical Fluid Chromatography–Mass-Spectrometry of Nitrogen-Containing Compounds: Atmospheric Pressure Ionization
AbstractSupercritical fluid chromatography (SFC) in combination with mass spectrometry is a promising alternative to traditional chromatographic...
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Selective Separation of Polar Unsaturated Organics Using a Water Stationary Phase in Gas Chromatography
A water stationary phase is explored as a novel means of selectively separating unsaturated analytes in gas chromatography. Several...
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Purification and Quality Control of Recombinant Proteins Expressed in Mammalian Cells: A Practical Review
In the recent years, there has been a rapid development of new technologies and strategies when it comes to protein purification and quality control...
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Methotrexate Polyglutamates Analysis by Chromatography Methods in Biological Matrices: A Review
Methotrexate (MTX) is used as an immunosuppressant and antineoplastic drug in clinical practice. MTX is a parent drug and converts to MTX...
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A Novel Adsorbent for Hydrophilic Chromatography Based on Silica Modified by the Ugi Reaction
AbstractNew stationary phases for hydrophilic chromatography (HILIC) with functional layers formed by the multicomponent Ugi reaction have been...
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Using solid-phase extraction to facilitate a focused tile-based Fisher ratio analysis of comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry data: comparative analysis of aerospace fuel composition
Tile-based Fisher ratio (F-ratio) analysis of comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC × GC-TOFMS) data...
