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Article
Open AccessChromosome-scale genome assembly of medicinal plant Tinospora sagittata (Oliv.) Gagnep. from the Menispermaceae family
Tinospora sagittata (Oliv.) Gagnep. is an important medicinal tetraploid plant in the Menispermaceae family. Its tuber, Radix Tinosporae, used in traditional Chinese medicine, is rich in diterpenoids and benzylis...
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Article
Effects of MhMYB1 and MhMYB2 transcription factors on the monoterpenoid biosynthesis pathway in l-menthol chemotype of Mentha haplocalyx Briq
Transcription factors MhMYB1 and MhMYB2 correlate with monoterpenoid biosynthesis pathway in l-menthol chemotype of Mentha haplocalyx Briq, which could affect the contents of ( −)-menthol and ( −)-menthone.
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Article
Open AccessExtraction and analysis of high-quality chloroplast DNA with reduced nuclear DNA for medicinal plants
Obtaining high-quality chloroplast genome sequences requires chloroplast DNA (cpDNA) samples that meet the sequencing requirements. The quality of extracted cpDNA directly impacts the efficiency and accuracy o...
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Article
Open AccessMining and functional characterization of NADPH-cytochrome P450 reductases of the DNJ biosynthetic pathway in mulberry leaves
1-Deoxynojirimycin (DNJ), the main active ingredient in mulberry leaves, with wide applications in the medicine and food industries due to its significant functions in lowering blood sugar, and lipids, and com...
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Article
Open AccessDoxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation
CRISPR-based toolkits have dramatically increased the ease of genome and epigenome editing. SpCas9 is the most widely used nuclease. However, the difficulty of delivering SpCas9 and inability to modulate its e...
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Article
Open AccessMultiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors
Cas12a can process multiple sgRNAs from a single transcript of CRISPR array, conferring advantages in multiplexed base editing when incorporated into base editor systems, which is extremely helpful given that ...
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Article
Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression
Inducible expression systems are indispensable for precise regulation and in-depth analysis of biological process. Binary Tet-On system has been widely employed to regulate transgenic expression by doxycycline...
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Article
Rayleigh-Bénard-Marangoni Instability in a Self-rewetting Fluid Layer Overlying a Porous Medium
Rayleigh-Bénard-Marangoni instability in a bilayer system of self-rewetting fluid overlying a porous medium is investigated. The upper surface of the fluid layer is assumed non-deformable and a constant temper...
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Article
Screening, cloning and functional characterization of key methyltransferase genes involved in the methylation step of 1-deoxynojirimycin alkaloids biosynthesis in mulberry leaves
The novel C-methyltransferase, MaMT1, could catalyze the conversion of piperidine to 2-methylpiperidine, which may be involved in the methylation step of DNJ biosynthesis in mulberry leaves.
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Article
Clean and SO2-Free Method for Bismuth Extraction from Bismuthinite by Multiphase Roasting: Thermodynamic Equilibria and Reaction Mechanisms
A clean method for bismuth extraction was investigated by multiphase roasting and beneficiation. Iron oxide and carbon, used as the sulfur-fixing agent and reductant, were roasted with Bi2S3, yielding Bi metal an...
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Article
Open AccessACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems
Many favorable traits of crops and livestock and human genetic diseases arise from multiple single nucleotide polymorphisms or multiple point mutations with heterogeneous base substitutions at the same locus. ...
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Article
Open AccessFrankincense and myrrh and their bioactive compounds ameliorate the multiple myeloma through regulation of metabolome profiling and JAK/STAT signaling pathway based on U266 cells
Frankincense and myrrh are used as traditional anti-inflammatory and analgesic medicines in China. It has been reported that frankincense and myrrh have significant anti-tumor activities. The present study was...
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Article
Removal of Iron from Polluted Tungsten Powder by Acid Leaching for Recovery of Tungsten
Iron-bearing tungsten powder generated during the reduction of tungsten oxide is an important secondary material. The aim of this study is to remove iron from polluted tungsten powder by acid leaching. First, ...
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Article
Clean Recycling Process for Lead Oxide Preparation from Spent Lead–Acid Battery Pastes Using Tartaric Acid–Sodium Tartrate as a Transforming Agent
A clean recycling process for waste lead–acid battery paste was proposed, where tartaric acid-sodium tartrate mixed solution was used as the transforming agent. First, lead tartrate [Pb(C4H4O6)] was prepared by t...
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Article
N6-(2-hydroxyethyl)-adenosine from Cordyceps cicadae attenuates hydrogen peroxide induced oxidative toxicity in PC12 cells
N6-(2-hydroxyethyl)-adenosine (HEA), is one of the active molecule found in Cordyceps cicadae. The protective effect of HEA against H2O2 induced oxidative damage in PC12 cells and the mechanism of action was inve...
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Article
Open AccessEfficient base editing for multiple genes and loci in pigs using base editors
Cytosine base editors (CBEs) enable programmable C-to-T conversion without DNA double-stranded breaks and homology-directed repair in a variety of organisms, which exhibit great potential for agricultural and ...
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Article
Open AccessBMI1 enables interspecies chimerism with human pluripotent stem cells
Human pluripotent stem cells (hPSCs) exhibit very limited contribution to interspecies chimeras. One explanation is that the conventional hPSCs are in a primed state and so unable to form chimeras in pre-impl...
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Article
Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems
CRISPR/Cpf1 features a number of properties that are distinct from CRISPR/Cas9 and provides an excellent alternative to Cas9 for genome editing. To date, genome engineering by CRISPR/Cpf1 has been reported only i...
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Article
Open AccessCharacterization and anti-tumor activity of a polysaccharide isolated from Dendrobium officinale grown in the Huoshan County
Polysaccharides are carbohydrate chains composed of linked monosaccharide units. Accumulating studies report that polysaccharides isolated from Dendrobium officinale have a variety of functions. However, the comp...
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Article
Open AccessExtrahepatic cytochrome P450s play an insignificant role in triptolide-induced toxicity
Triptolide, an active ingredient of Chinese medicine plant Tripterygium wilfordii Hook.f., has been shown to exert anti-tumor, immunosuppressive, anti-inflammatory, and anti-fertility pharmacological effects. How...
