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Article
Open AccessExtraction and analysis of high-quality chloroplast DNA with reduced nuclear DNA for medicinal plants
Obtaining high-quality chloroplast genome sequences requires chloroplast DNA (cpDNA) samples that meet the sequencing requirements. The quality of extracted cpDNA directly impacts the efficiency and accuracy o...
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Article
Transcriptome Analysis of Nitrogen Metabolism, Transcription Factors, and Indigoid Biosynthesis in Isatis indigotica Fort. Response to Nitrogen Availability
Isatis indigotica Fort. is a popular herb in traditional Chinese medicine. It possesses pharmacological activities against various diseases, particularly leukemia. Indigoid alkaloids are its main bioactive ingred...
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Article
Open AccessSelection of reference genes for the quantitative real-time PCR normalization of gene expression in Isatis indigotica fortune
Isatis indigotica, a traditional Chinese medicine, produces a variety of active ingredients. However, little is known about the key genes and corresponding expression profiling involved in the biosynthesis pathwa...