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  1. Article

    Open Access

    Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation

    CRISPR-based toolkits have dramatically increased the ease of genome and epigenome editing. SpCas9 is the most widely used nuclease. However, the difficulty of delivering SpCas9 and inability to modulate its e...

    Qin **, **aoyi Liu, Zhenpeng Zhuang, Jiayuan Huang, Shixue Gou, Hui Shi… in Genome Biology (2023)

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  2. No Access

    Article

    Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression

    Inducible expression systems are indispensable for precise regulation and in-depth analysis of biological process. Binary Tet-On system has been widely employed to regulate transgenic expression by doxycycline...

    Qin **, **aoyu Yang, Shixue Gou, **aoyi Liu… in Science China Life Sciences (2022)

  3. Article

    Open Access

    ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems

    Many favorable traits of crops and livestock and human genetic diseases arise from multiple single nucleotide polymorphisms or multiple point mutations with heterogeneous base substitutions at the same locus. ...

    **gke **e, **ngyun Huang, **a Wang, Shixue Gou, Yanhui Liang, Fangbing Chen… in BMC Biology (2020)

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