176 Result(s)
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Article
Porous kaolinite bead: A novel but economical solid support material for biomass in fluidised bed technology
Porous kaolinite beads were prepared by mixing kaolinite with various proportions of rice hull ash (RHA) and heating in a furnace at 800 °C for five hours. Those with 10 to 60% (w/w) RHA were very porous and h...
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Chapter
Synthetic Peptides and the Design of Peptide and Protein Recognition Surfaces
Macromolecular recognition is a unifying theme in biology and biotechnology and understanding the mechanisms underlying this phenomenon remain important goals of biochemical study. The structural nature of rec...
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Chapter
Methanogenesis from Propionate in Sludge and Enrichment Systems
The biological formation of methane from organic matter is a complex microbiological process involving many physiologically dependent relationships between and among a diversity of heterotrophic fermentative a...
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Article
Female sex pheromone of oriental tobacco budworm,Helicoverpa assulta (Guenee) (Lepidoptera: Noctuidae): Identification and field testing
Analysis of ovipositor washings from virgin femaleHelicoverpa assulta (Guenée) (Lepidoptere: Noctuidae) from Korea by gas chromatography (GC) linked to electroantennography and GC linked to mass spectrometry resu...
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Chapter
Biochemical and Molecular Biological Characterization of Biotinylated Proteins of Plants
At least six biotinylated polypeptides can be detected in extracts from plants (1). These polypeptides are approximately 240 kDa, 120 kDa, 75 kDa, 60 kDa, 39 kDa and 34 kDa in size. Furthermore, the biotin enz...
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Chapter
Study on the Acrtion Mechanism of Hemorrhagin I from Agkistrodon acutus Venom
Up to now, studies on the receptor of neural toxins and their functional mechanism were extensively reported (Tu, 1977a). Receptors of neural toxins have been proved to be present in the animal’s cells. As to ...
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Chapter and Conference Paper
Analysis of Cell Death by Flow Cytometry
Apoptosis and necrosis denote two distinct modes of cell death. The distinction is based on differences in morphological, biochemical and molecular changes that occur within the dying cell (revie...
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Article
Purification, characterization and gene sequence analysis of a novel cytochrome c co-induced with reductive dechlorination activity in Desulfomonile tiedjei DCB-1
The sulfate-reducing bacterium, Desulfomonile tiedjei DCB-1, conserves energy for growth from reductive dechlorination of 3-chlorobenzoate via halorespiration. To understand this respiratory process better, we ex...
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Chapter
Directional mutational pressure affects the amino acid composition and hydrophobicity of proteins in bacteria
The relationship between change in genomic GC content and protein evolution in bacteria was studied by simple correlational analysis (at the genus level) and by Felsenstein’s (1985) independent contrast test. ...
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Article
Novel Biological Process for L-DOPA Production from L-Tyrosine by P-Hydroxyphenylacetate 3-Hydroxylase
A novel biological method was developed for the production of L-3,4-dihydroxyphenylalanine (L-DOPA) from L-tyrosine by p-hydroxyphenylacetate 3-hydroxylase of Escherichia coli strain W (ATCC 11105). About 48 m...
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Chapter
Characterization and identification of growth hormone receptors in snakeheaded fish (Ophiocephalida argus cantor) liver membrane
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Chapter
Effect of divalent cations and disulfide reducing agents on the specific binding of growth hormone to snakeheaded fish liver membrane
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Article
Structure of the Bcr-Abl oncoprotein oligomerization domain
The Bcr-Abl oncoprotein is responsible for a wide range of human leukemias, including most cases of Philadelphia chromosome-positive chronic myelogenous leukemia. Oligomerization of Bcr-Abl is essential for on...
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Chapter
Functional divergence in protein (family) sequence evolution
As widely used today to infer ‘:function’, the homology search is based on the neutral theory that sites of greatest functional significance are under the strongest selective constraints as well as lowest evol...
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Protocol
Microscale Solution Isoelectrofocusing
Proteomics is a relatively new research discipline that deals with systematic, large-scale analyses of proteins in biological systems (1,2). Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) is curren...
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Article
Cloning and localization of vip3A gene of Bacillus thuringiensis
An insecticidal protein gene, vip3A, was cloned from Bacillus thuringiensis strain WB50. The nucleotide sequence of 2,460 bp (GenBank acc. No. AY295778) showed 99% homology with the known vip3A genes. Using speci...
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Article
Expression, Purification and Characterization of a Recombinant β-Glucosidase from Volvariella Volvacea
For the first time, a β-glucosidase gene from the edible straw mushroom, Volvariella volvacea V1-1, has been over-expressed in E. coli. The gene product was purified by chromatography showing a single band on SDS...
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Article
Enhanced Expression and Purification of Membrane Proteins by SUMO Fusion in Escherichia coli
Severe acute respiratory syndrome coronavirus (SARS-CoV) membrane protein and 5-lipoxygenase-activating protein (FLAP) are among a large number of membrane proteins that are poorly expressed when traditional e...
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Article
Assembly of Plasmid DNA into Liposomes After Condensation by Cationic Lipid in Anionic Detergent Solution
A novel strategy to prepare negatively charged and small DNA-containing liposomes after condensation of plasmid DNA by a cationic lipid in deoxycholate micelle environment is described. The average diameter of...
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Article
Expression of Galactose Permease and Pyruvate Carboxylase in Escherichia coli ptsG Mutant Increases the Growth Rate and Succinate Yield under Anaerobic Conditions
In Escherichia coli, disruption of ptsG, which encodes the glucose-specific permease of the phosphotransferase transport system (PTS) protein EIICBGlc, is crucial for high succinate production. This mutation can,...
