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Article
Influence of media selection on NMR based metabolic profiling of human cell lines
Comparative metabolic profiling of different human cancer cell lines can reveal metabolic pathways up-regulated or down-regulated in each cell line, potentially providing insight into distinct metabolism takin...
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Protocol
Applications of NMR-Based PRE and EPR-Based DEER Spectroscopy to Homodimer Chain Exchange Characterization and Structure Determination
The success of homodimer structure determination by conventional solution NMR spectroscopy relies greatly on interchain distance restraints (less than 6 Å) derived from nuclear Overhauser effects (NOEs) obtain...
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Article
Measurement of rate constants for homodimer subunit exchange using double electron–electron resonance and paramagnetic relaxation enhancements
Here, we report novel methods to measure rate constants for homodimer subunit exchange using double electron–electron resonance (DEER) electron paramagnetic resonance spectroscopy measurements and nuclear magn...
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Article
Identification of a plant-specific Zn2+-sensitive ribonuclease activity
Ribonucleases (RNases) play a variety of cellular and biological roles in all three domains of life. In an attempt to perform RNA immuno-precipitation assays of Arabidopsis proteins, we found an EDTA-dependent...
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Article
NMR resonance assignments of the human high mobility group protein HMGA1
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Article
Purification, characterization and gene sequence analysis of a novel cytochrome c co-induced with reductive dechlorination activity in Desulfomonile tiedjei DCB-1
The sulfate-reducing bacterium, Desulfomonile tiedjei DCB-1, conserves energy for growth from reductive dechlorination of 3-chlorobenzoate via halorespiration. To understand this respiratory process better, we ex...
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Article
Human nucleotide excision repair protein XPA: Expression and NMR backbone assignments of the 14.7 kDa minimal damaged DNA binding domain (Met98–Phe219)
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Chapter
Catabolism of Dimethylsulfide and Methane Thiol by Methylotrophic Methanogens
We demonstrated the ability of several methylotrophic methanogens to degrade dimethylsulfide and methane thiol to hydrogen sulfide, methane, and carbon dioxide. This is the first report of the growth of a pure...