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Protocol
Advanced Fusion Strategies for the Production of Functionalized Potato Virus X Virions
Plant virions are ideal for nanotechnology applications because they are structurally diverse and can self-assemble naturally, allowing for large-scale production in plants by molecular farming. Potato virus X...
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Protocol
In Planta Production of Fluorescent Filamentous Plant Virus-Based Nanoparticles
Viral nanoparticles are attractive platforms for biomedical applications and are frequently employed for optical imaging in tissue culture and preclinical animal models as fluorescent probes. Chemical modifica...
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Protocol
Bioinspired Silica Mineralization on Viral Templates
Plant virus capsids are attractive entities for nanotechnological applications because of their variation in shape and natural assembly ability. This chapter describes the production and modification of three ...
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Article
Open AccessHeterologous expression of a Streptomyces cyaneus laccase for biomass modification applications
Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing S...
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Article
Open AccessCellulolytic RoboLector – towards an automated high-throughput screening platform for recombinant cellulase expression
Cellulases are key player in the hydrolyzation of cellulose. Unfortunately, this reaction is slow and a bottleneck in the process chain from biomass to intermediates and biofuels due to low activities of the e...
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Article
Open AccessTo Target or Not to Target: Active vs. Passive Tumor Homing of Filamentous Nanoparticles Based on Potato virus X
Nanoparticles are promising platforms for the diagnosis and treatment of cancer. Diverse classes and shapes of materials have been investigated to establish design principles that achieve the effective partiti...
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Article
Open AccessCell wall modification in tobacco by differential targeting of recombinant endoglucanase from Trichoderma reesei
The development of transgenic plants as a production platform for biomass-degrading enzymes is a promising tool for an economically feasible allocation of enzymes processing lignocellulose. Previous research h...
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Chapter and Conference Paper
The Cellulolytic System of Cyst Nematodes
The growing energy consumption and the dwindling resources of fossil energy carriers make the exploitation of renewable energy from biomass a key technology for the maintenance of modern industrial societies a...
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Chapter and Conference Paper
Co-expression of Cellulases in the Chloroplasts of Nicotiana tabacum
Due to the increasing demand for alternative energy carriers, biomass is in the focus of research and industry as raw material for fuels and base chemicals. This has led to an increased use of feedstock for bi...
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Article
Open AccessChallenges and advances in the heterologous expression of cellulolytic enzymes: a review
Second generation biofuel development is increasingly reliant on the recombinant expression of cellulases. Designing or identifying successful expression systems is thus of preeminent importance to industrial ...
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Protocol
Genetic Engineering and Chemical Conjugation of Potato Virus X
Here we report the genetic engineering and chemical modification of potato virus X (PVX) for the presentation of various peptides, proteins, and fluorescent dyes, or other chemical modifiers. Three different w...
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Article
Open AccessEthanol inducible expression of a mesophilic cellulase avoids adverse effects on plant development
Plant-produced biomass-degrading enzymes are promising tools for the processing of lignocellulose to fermentable sugars. A major limitation of in planta production is that high-level expression of such enzymes co...
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Article
Open AccessHyperthermophilic endoglucanase for in planta lignocellulose conversion
The enzymatic conversion of lignocellulosic plant biomass into fermentable sugars is a crucial step in the sustainable and environmentally friendly production of biofuels. However, a major drawback of enzymes ...
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Article
In vivo expression and binding activity of scFv-RWAV, which recognizes the coat protein of tomato leaf curl New Delhi virus (family Geminiviridae)
Recombinant antibodies expressed in plants have the potential to interrupt virus infections by blocking essential stages of the infection cycle. Here, we show that the expression of a recombinant single-chain ...
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Article
Open AccessHow recombinant swollenin from Kluyveromyces lactisaffects cellulosicsubstrates and accelerates their hydrolysis
In order to generate biofuels, insoluble cellulosic substrates are pretreated andsubsequently hydrolyzed with cellulases. One way to pretreat cellulose in a safeand environmentally friendly manner is to apply,...
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Article
Generation and characterization of a scFv against recombinant coat protein of the geminivirus tomato leaf curl New Delhi virus
We report the establishment of a hybridoma cell line secreting the monoclonal antibody (mAb) HAV, which recognizes the coat (AV1) protein of tomato leaf curl New Delhi virus (ToLCNDV), a begomovirus. The cell ...
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Article
Recombinant-antibody-mediated resistance against Tomato yellow leaf curl virus in Nicotiana benthamiana
Tomato yellow leaf curl virus (TYLCV) is a geminivirus species whose members cause severe crop losses in the tropics and subtropics. We report the expression of a single-chain variable fr...
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Article
The Potential of Plant Virus Vectors for Vaccine Production
Plants viruses are versatile vectors that allow the rapid and convenient production of recombinant proteins in plants. Compared with production systems based on transgenic plants, viral vectors are easier to m...
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Article
Expression of single-chain antibody fragments (scFv) specific for beet necrotic yellow vein virus coat protein or 25 kDa protein in Escherichia coli and Nicotiana benthamiana
The coding sequences for the variable regions of heavy and light chains of monoclonal antibodies (mAbs) to beet necrotic yellow vein virus (BNYVV) coat protein (cp) or the 25 kDa nonstructural protein (P25) we...
