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Article
Open AccessIsolation and light chain shuffling of a Plasmodium falciparum AMA1-specific human monoclonal antibody with growth inhibitory activity
Plasmodium falciparum, the parasite causing malaria, affects populations in many endemic countries threatening mainly individuals with low malaria immunity, especially children. Despite the approval of the first ...
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Chapter and Conference Paper
Sensitivity Analysis for Resilient Safety Design: Application to a Bottleneck Scenario
Microscopic crowd simulations have become an essential tool to devise safety strategies within built environments. Evacuating a building means guiding pedestrians through a series of corridors and doors, that ...
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Chapter
Produkt-Controlling
Zentraler Baustein des Marketing ist die „facettenreiche“ Produktpolitik. Im nachstehenden Beitrag wird zunächst die Einordnung der Produktpolitik in den Zielkatalog des Marketing und des Unternehmens skizzier...
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Chapter
Glyco-Engineering of Plant-Based Expression Systems
Most secreted proteins in eukaryotes are glycosylated, and after a number of common biosynthesis steps the glycan structures mature in a species-dependent manner. Therefore, human therapeutic proteins produced...
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Article
Improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast cell walls
The enzymatic degradation of azo dyes is a promising alternative to ineffective chemical and physical remediation methods. Lignin peroxidase (LiP) from Phanerochaete chrysosporium is a heme-containing lignin-degr...
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Article
Semi-rational design of cellobiose dehydrogenase for increased stability in the presence of peroxide
Cellobiose dehydrogenase (CDH, EC 1.1.99.18) from white rot fungi Phanerochaete chrysosporium can be used for constructing biosensors and biofuel cells, for bleaching cotton in textile industry, and recently, the...
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Article
Open AccessGene expression variability between randomly and targeted transgene integration events in tobacco suspension cell lines
Genome editing tools such as zinc-finger nucleases provide novel strategies for genetic manipulation in plants. Unlike agrobacterium-mediated or direct gene transfer, which introduce genes randomly into the ge...
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Chapter and Conference Paper
Towards Inferring Input Parameters from Measurements: Bayesian Inversion for a Bottleneck Scenario
Microscopic crowd can help to enhance the safety of pedestrians in various situations, provided that the simulation yields realistic results. One crucial step on the way toward realistic simulations is cali...
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Article
Open AccessInvestigation of the methylerythritol 4-phosphate pathway for microbial terpenoid production through metabolic control analysis
Terpenoids are of high interest as chemical building blocks and pharmaceuticals. In microbes, terpenoids can be synthesized via the methylerythritol phosphate (MEP) or mevalonate (MVA) pathways. Although the M...
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Chapter and Conference Paper
Phenomenology and analysis of gas pressures at low-speed pre-ignitions
The phenomenon of low-speed pre-ignition (LSPI) has gained widespread significance with the growing implementation of downsizing concepts in gasoline engines. Failures of piston top land, second land or skirt ...
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Article
Characterization of new anti-IL-6 antibodies revealed high potency candidates for intracellular cytokine detection and specific targeting of IL-6 receptor binding sites
Interleukin-6 (IL-6) expression and secretion, induced by inflammatory processes, stimulate the acute phase response cascade. The overexpression of IL-6 contributes to a variety of inflammatory diseases, e.g. ...
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Protocol
In Planta Production of Fluorescent Filamentous Plant Virus-Based Nanoparticles
Viral nanoparticles are attractive platforms for biomedical applications and are frequently employed for optical imaging in tissue culture and preclinical animal models as fluorescent probes. Chemical modifica...
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Article
Efficient targeting of CD13 on cancer cells by the immunotoxin scFv13–ETA′ and the bispecific scFv [13xds16]
Treatment of cancer using standard chemotherapy still offers a poor prognosis combined with severe side effects. Novel antibody-based therapies have been shown to overcome low efficiency and lack of selectivit...
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Article
Novel PSCA targeting scFv-fusion proteins for diagnosis and immunotherapy of prostate cancer
Despite great progress in the diagnosis and treatment of localized prostate cancer (PCa), there remains a need for new diagnostic markers that can accurately distinguish indolent and aggressive variants. One p...
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Article
Open AccessImprovement of a fermentation process for the production of two PfAMA1-DiCo-based malaria vaccine candidates in Pichia pastoris
Pichia pastoris is a simple and powerful expression platform that has the ability to produce a wide variety of recombinant proteins, ranging from simple peptides to complex membrane prote...
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Article
Open AccessThe digestive and defensive basis of carcass utilization by the burying beetle and its microbiota
Insects that use ephemeral resources must rapidly digest nutrients and simultaneously protect them from competitors. Here we use burying beetles (Nicrophorus vespilloides), which feed their offspring on vertebrat...
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Article
Open AccessHeterologous expression of a Streptomyces cyaneus laccase for biomass modification applications
Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing S...
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Article
Detection and Specific Elimination of EGFR+ Ovarian Cancer Cells Using a Near Infrared Photoimmunotheranostic Approach
Targeted theranostics is an alternative strategy in cancer management that aims to improve cancer detection and treatment simultaneously. This approach combines potent therapeutic and diagnostic agents with th...
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Chapter
Produkt-Controlling
Zentraler Baustein des Marketing ist die „facettenreiche“ Produktpolitik. Im nachstehenden Beitrag wird zunächst die Einordnung der Produktpolitik in den Zielkatalog des Marketing und des Unternehmens skizzier...
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Article
Open AccessCharacterization of a novel inhibitory human monoclonal antibody directed against Plasmodium falciparum Apical Membrane Antigen 1
Malaria remains a major challenge to global health causing extensive morbidity and mortality. Yet, there is no efficient vaccine and the immune response remains incompletely understood. Apical Membrane Antigen...
