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  1. No Access

    Article

    Wheat yellow mosaic virus resistance in wheat cultivar Madsen acts in roots but not in leaves

    Wheat yellow mosaic virus (WYMV), a member of Bymovirus, is a plant pathogenic virus transmitted by the soil-borne plasmodiophorid Polymyxa graminis. The in planta titer of the virus must...

    Cheng Liu, Takako Suzuki, Kohei Mishina… in Journal of General Plant Pathology (2016)

  2. No Access

    Article

    A new sequence variant of wheat spindle streak mosaic virus in Germany

    Wheat spindle streak mosaic virus is a Bymovirus transmitted by the plasmodiophorid Polymyxa graminis that infects small grains such as wheat, rye and triticale. Nucleotide sequences of WSSMV isolates from severa...

    Angelika Ziegler, Ute Kastirr in Journal of Plant Diseases and Protection (2016)

  3. No Access

    Article

    First detection of wheat streak mosaic virus in Germany: molecular and biological characteristics

    Wheat streak mosaic virus is a serious threat in wheat-producing countries. In Germany, the virus was first recorded in 2013 near Hoym. The complete sequence of isolate Hoym was obtained and compared to all ot...

    Jörg Schubert, Angelika Ziegler, Frank Rabenstein in Archives of Virology (2015)

  4. No Access

    Article

    Quantification of Wheat spindle streak mosaic virus and Soil borne cereal mosaic virus in resistance testing of field samples of triticale using real-time RT-PCR

    Triticale, a cross between rye and wheat, is a crop important for animal feed and the production of biogas and ethanol. Soil-borne viruses found in wheat and rye, such as Furoviruses and Bymoviruses, also infe...

    Angelika Ziegler, Karin Klingebeil, Viola Papke… in Journal of Plant Diseases and Protection (2014)

  5. No Access

    Article

    In vivo expression and binding activity of scFv-RWAV, which recognizes the coat protein of tomato leaf curl New Delhi virus (family Geminiviridae)

    Recombinant antibodies expressed in plants have the potential to interrupt virus infections by blocking essential stages of the infection cycle. Here, we show that the expression of a recombinant single-chain ...

    Adel M. Zakri, Angelika Ziegler, Ulrich Commandeur, Rainer Fischer… in Archives of Virology (2012)

  6. No Access

    Article

    Complete sequence of a cryptic virus from hemp (Cannabis sativa)

    Hemp (Cannabis sativa) was found to be a useful propagation host for hop latent virus, a carlavirus. However, when virus preparations were analysed by electron microscopy, along with the expected filamentous part...

    Angelika Ziegler, Jaroslav Matoušek, Gerhard Steger, Jörg Schubert in Archives of Virology (2012)

  7. No Access

    Article

    Generation and characterization of a scFv against recombinant coat protein of the geminivirus tomato leaf curl New Delhi virus

    We report the establishment of a hybridoma cell line secreting the monoclonal antibody (mAb) HAV, which recognizes the coat (AV1) protein of tomato leaf curl New Delhi virus (ToLCNDV), a begomovirus. The cell ...

    Adel M. Zakri, Angelika Ziegler, Lesley Torrance, Rainer Fischer… in Archives of Virology (2010)

  8. No Access

    Article

    Comparative sequence analysis and serological and infectivity studies indicate that cocksfoot mild mosaic virus is a member of the genus Panicovirus

    The complete nucleotide sequence of the Phleum isolate of cocksfoot mild mosaic virus (CMMV-P) and the coat protein sequence of the cocksfoot isolate (CMMV-1) were determined. Comparative sequence analysis reveal...

    Angelika Ziegler, Graham Cowan, Lesley Torrance in Archives of Virology (2009)

  9. No Access

    Protocol

    Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP Fusions

    This chapter describes techniques to investigate the localisation and function of virus-encoded proteins in plants using green fluorescent protein (GFP) transiently expressed from plasmids or infectious cDNA r...

    Sophie Haupt, Angelika Ziegler, Graham Cowan… in Viral Applications of Green Fluorescent Pr… (2009)

  10. No Access

    Protocol

    Localization of Viral Proteins in Plant Cells: Protein Tagging

    This chapter describes techniques for in vivo imaging of fluorescent fusion proteins in living cells by confocal laser scanning microscopy (CLSM). Methods are provided for (i) producing the constructs for tran...

    Sophie Haupt, Angelika Ziegler, Lesley Torrance in Plant Virology Protocols (2008)

  11. No Access

    Article

    Facile assessment of cDNA constructs for expression of functional antibodies in plants using the potato virus X vector

    Antibodies have been expressed in plants to confer novel traits such as virus resistance or altered phenotype. However, not every antibody is suitable for plant expression, and successful intracellular express...

    Angelika Ziegler, Graham H. Cowan, Lesley Torrance, Heather A. Ross… in Molecular Breeding (2000)

  12. No Access

    Article

    Recombinant antibody fragments that detect enoyl acyl carrier protein reductase in Brassica napus

    Purified Brassica napus enoyl acyl carrier protein reductase (ENR) was used to select specific antibodies from a library of antibody fragments, single-chain Fv (scFv), displayed on filamentous phage. Analysis of ...

    Angelika Ziegler, Sybil M. Macintosh, Lesley Torrance, William Simon… in Lipids (1997)