24 Result(s)
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Article
Open AccessQuantification methods of determining brewer’s and pharmaceutical yeast cell viability: accuracy and impact of nanoparticles
For industrial processes, a fast, precise, and reliable method of determining the physiological state of yeast cells, especially viability, is essential. However, an increasing number of processes use magnetic...
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Article
Author Correction: A multipoint guidance mechanism for β-barrel folding on the SAM complex
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A multipoint guidance mechanism for β-barrel folding on the SAM complex
Mitochondrial β-barrel proteins are essential for the transport of metabolites, ions and proteins. The sorting and assembly machinery (SAM) mediates their folding and membrane insertion. We report the cryo-ele...
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Article
Quality control of the mitochondrial proteome
Mitochondria contain about 1,000–1,500 proteins that fulfil multiple functions. Mitochondrial proteins originate from two genomes: mitochondrial and nuclear. Hence, proper mitochondrial function requires synch...
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Article
Online sensor validation in sensor networks for bioprocess monitoring using swarm intelligence
Sensor faults can impede the functionality of monitoring and control systems for bioprocesses. Hence, suitable systems need to be developed to validate the sensor readings prior to their use in monitoring and ...
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Article
RQT complex dissociates ribosomes collided on endogenous RQC substrate SDD1
Ribosome-associated quality control (RQC) represents a rescue pathway in eukaryotic cells that is triggered upon translational stalling. Collided ribosomes are recognized for subsequent dissociation followed b...
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Article
Structure of the Bcs1 AAA-ATPase suggests an airlock-like translocation mechanism for folded proteins
Some proteins require completion of folding before translocation across a membrane into another cellular compartment. Yet the permeability barrier of the membrane should not be compromised and mechanisms have ...
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Mitochondrial porin links protein biogenesis to metabolism
In this report, we summarize recent findings about a role of the outer membrane metabolite channel VDAC/porin in protein import into mitochondria. Mitochondria fulfill key functions for cellular energy metabol...
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Article
Structure of the 80S ribosome–Xrn1 nuclease complex
Messenger RNA (mRNA) homeostasis represents an essential part of gene expression, in which the generation of mRNA by RNA polymerase is counter-balanced by its degradation by nucleases. The conserved 5′-to-3′ e...
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Ribosome–NatA architecture reveals that rRNA expansion segments coordinate N-terminal acetylation
The majority of eukaryotic proteins are N-terminally α-acetylated by N-terminal acetyltransferases (NATs). Acetylation usually occurs co-translationally and defects have severe consequences. Nevertheless, it i...
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Article
Open AccessThe novel TRAIL-receptor agonist APG350 exerts superior therapeutic activity in pancreatic cancer cells
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has raised attention as a novel anticancer therapeutic as it induces apoptosis preferentially in tumor cells. However, first-generation TRAIL-rec...
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Article
Characterization of polymeric substance classes in cereal-based beverages using asymmetrical flow field-flow fractionation with a multi-detection system
Cereal-based beverages contain a complex mixture of various polymeric macromolecules including polysaccharides, peptides, and polyphenols. The molar mass of polymers and their degradation products affect diffe...
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Article
Structure of the 40S–ABCE1 post-splitting complex in ribosome recycling and translation initiation
Cryo-EM structures of the yeast 40S in complex with ribosome-splitting protein ABCE1, along with functional analyses, reveal that the FeS cluster domain undergoes a 150° rotation to dissociate ribosomal subunits.
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Article
Rapid cultivar identification of barley seeds through disjoint principal component modeling
Classification of barley varieties is a crucial part of the control and assessment of barley seeds especially for the malting and brewing industry. The correct classification of barley is essential in that a m...
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Article
How lipids modulate mitochondrial protein import
Mitochondria have to import the vast majority of their proteins, which are synthesized as precursors on cytosolic ribosomes. The translocase of the outer membrane (TOM complex) forms the general entry gate for...
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Mitochondriale Systeme für den Import von Proteinen
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Structure of the no-go mRNA decay complex Dom34–Hbs1 bound to a stalled 80S ribosome
Messenger RNAs (mRNAs) that induce stalling during translation are degraded by a quality control mechanism known as no-go decay (NGD). The aberrant mRNAs are recognized by two factors, Dom34 and Hbs1. Using cr...
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Article
Cryo-EM structure of the ribosome–SecYE complex in the membrane environment
A cryo-EM structure of the bacterial ribosome–SecYEG complex in a so-called Nanodisc allows for the molecular interpretation of the SecYEG complex in its natural lipid bilayer environment. Molecular dynamics s...
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Article
Common ground for protein translocation: access control for mitochondria and chloroplasts
The vast majority of mitochondrial and chloroplast proteins are cytosolically synthesized and have to be translocated into the organelle.
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Future Aspects of Bioprocess Monitoring
Nature has the impressive ability to efficiently and precisely control biological processes by applying highly evolved principles and using minimal space and relatively simple building blocks. The challenge...
