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Correction to: Evaluation of CHROMagar™‑Serratia agar, a new chromogenic medium for the detection and isolation of Serratia marcescens
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Protocol
PLASmid TAXonomic PCR (PlasTax-PCR), a Multiplex Relaxase MOB Ty** to Assort Plasmids into Taxonomic Units
Plasmids transmissible by conjugation are responsible for disseminating antibiotic-resistance genes, making plasmid detection relevant for pathogen tracking. We describe the use of a multiplex PCR method for t...
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Open AccessEvaluation of CHROMagar™-Serratia agar, a new chromogenic medium for the detection and isolation of Serratia marcescens
A comparative analysis of the performance of the new selective chromogenic CHROMagar™-Serratia culture medium for detection and isolation of Serratia marcescens was undertaken. A total of 134 clinical isolates (9...
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Article
Open AccessThe global dissemination of hospital clones of Enterococcus faecium
The hospital-adapted A1 group of Enterococcus faecium remains an organism of significant concern in the context of drug-resistant hospital-associated infections. How this pathogen evolves and disseminates remains...
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Open AccessApparent nosocomial adaptation of Enterococcus faecalis predates the modern hospital era
Enterococcus faecalis is a commensal and nosocomial pathogen, which is also ubiquitous in animals and insects, representing a classical generalist microorganism. Here, we study E. faecalis isolates ranging from t...
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Statistical Analysis of Accessory Genome
AcCNET (Accessory genome Constellation Network) is a Perl application to determine the variation of content in any genomic unit (e.g., bacterial genomes, plasmids, and transposons) within large datasets. It pr...
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Protocol
Methods to Quantify DNA Transfer in Enterococcus
DNA uptake in Enterococcus normally occurs by conjugation, a natural process that is replicated in biomedical research to assess the transferability of different mobile genetic elements and chromosomal regions as...
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Isolation and Visualization of Plasmids from Gram-Positive Bacteria of Interest in Public Health
This chapter describes the methods to extract and characterize plasmids of Gram-positive bacterial species of interest in public health (biomedicine, veterinary, and food safety) as Staphylococcus, Streptococcus,...
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Article
Defining and combating antibiotic resistance from One Health and Global Health perspectives
Several interconnected human, animal and environmental habitats can contribute to the emergence, evolution and spread of antibiotic resistance, and the health of these contiguous habitats (the focus of the One...
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Article
Prediction of the intestinal resistome by a three-dimensional structure-based method
The intestinal microbiota is considered to be a major reservoir of antibiotic resistance determinants (ARDs) that could potentially be transferred to bacterial pathogens via mobile genetic elements. Yet, this ...
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Open AccessIn-depth resistome analysis by targeted metagenomics
Antimicrobial resistance is a major global health challenge. Metagenomics allows analyzing the presence and dynamics of “resistomes” (the ensemble of genes encoding antimicrobial resistance in a given microbio...
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Article
Open AccessMultiple adaptive routes of Salmonella enterica Typhimurium to biocide and antibiotic exposure
Biocides and antibiotics are used to eradicate or prevent the growth of microbial species on surfaces (occasionally on catheters), or infected sites, either in combination or sequentially, raising concerns abo...
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Open AccessErratum to: Investigating the mobilome in clinically important lineages of Enterococcus faecium and Enterococcus faecalis
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Open AccessA membrane computing simulator of trans-hierarchical antibiotic resistance evolution dynamics in nested ecological compartments (ARES)
Antibiotic resistance is a major biomedical problem upon which public health systems demand solutions to construe the dynamics and epidemiological risk of resistant bacteria in anthropogenically-altered enviro...
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Prioritizing risks of antibiotic resistance genes in all metagenomes
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Open AccessInvestigating the mobilome in clinically important lineages of Enterococcus faecium and Enterococcus faecalis
The success of Enterococcus faecium and E. faecalis evolving as multi-resistant nosocomial pathogens is associated with their ability to acquire and share adaptive traits, including antimicrobial resistance genes...
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Article
What is a resistance gene? Ranking risk in resistomes
In this Opinion article, Baquero and colleagues propose a hierarchical system for estimating the risks associated with genes present in environmental resistomes, by evaluating the likelihood of their introduct...
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Open AccessDiversity and biofilm-production ability among isolates of Escherichia coli phylogroup D belonging to ST69, ST393 and ST405 clonal groups
Phylogenetic group D Escherichia coli clones (ST69, ST393, ST405) are increasingly reported as multidrug resistant strains causing extra-intestinal infections. We aim to characterize inter- and intraclonal divers...
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Protocol
Application of Molecular Techniques to the Study of Nosocomial Infections Caused by Enterococci
Enterococci are components of the normal bowel flora of humans and other animals, and have traditionally been considered to be of relatively low virulence in healthy individuals. However, they are increasingly...
