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  1. Article

    Open Access

    Apparent nosocomial adaptation of Enterococcus faecalis predates the modern hospital era

    Enterococcus faecalis is a commensal and nosocomial pathogen, which is also ubiquitous in animals and insects, representing a classical generalist microorganism. Here, we study E. faecalis isolates ranging from t...

    Anna K. Pöntinen, Janetta Top, Sergio Arredondo-Alonso… in Nature Communications (2021)

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  2. No Access

    Protocol

    Methods to Quantify DNA Transfer in Enterococcus

    DNA uptake in Enterococcus normally occurs by conjugation, a natural process that is replicated in biomedical research to assess the transferability of different mobile genetic elements and chromosomal regions as...

    Carla Novais, Ana R. Freitas, Ricardo León-Sampedro… in Horizontal Gene Transfer (2020)

  3. No Access

    Protocol

    Isolation and Visualization of Plasmids from Gram-Positive Bacteria of Interest in Public Health

    This chapter describes the methods to extract and characterize plasmids of Gram-positive bacterial species of interest in public health (biomedicine, veterinary, and food safety) as Staphylococcus, Streptococcus,...

    Ana R. Freitas, Carla Novais, Luísa Peixe, Teresa M. Coque in Horizontal Gene Transfer (2020)