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Article
Molecular cloning and characterization of a novel gene encoding zinc finger protein from Medicago sativa L.
A suppression subtraction hybridization (SSH) cDNA library had been constructed to identify differentially expressed genes. Based on the sequence of an expressed sequence tag (EST) homologous to Pisum sativum zin...
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Article
Molecular cloning and characterization of three isoprenyl diphosphate synthase genes from alfalfa
Isoprenoid is the precursor for the biosynthesis of saponins, abscisic acid, gibberellins, chlorophylls and many other products in plants. Saponins are an important group of bioactive plant natural products. T...
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Article
Overexpression of a novel salt stress-induced glycine-rich protein gene from alfalfa causes salt and ABA sensitivity in Arabidopsis
We cloned a novel salt stress-induced glycine-rich protein gene ( MsGRP ) from alfalfa. Its overexpression retards seed germination and seedling growth of tr...
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Article
Molecular cloning and functional analysis of a salt-induced gene encoding an RNA-binding protein in alfalfa
Based on the sequence of an expressed sequence tag of alfalfa (Medicago sativa L. cv. Zhongmu-1), a full-length sequence of 1,551 nucleotides was isolated using RACE-PCR techniques. This gene (MsRBP) was predicte...
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Article
Isolation and functional characterization of salt-stress induced RCI2-like genes from Medicago sativa and Medicago truncatula
Salt stress is one of the most significant adverse abiotic factors, causing crop failure worldwide. So far, a number of salt stress-induced genes, and genes improving salt tolerance have been characterized in ...
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Article
Molecular cloning and functional analysis of the drought tolerance gene MsHSP70 from alfalfa (Medicago sativa L.)
Heat shock proteins (HSPs) are a ubiquitously expressed class of protective proteins that play a key role in plant response to stressful conditions. This study aimed to characterize and investigate the functio...
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Article
Comparative proteomic analysis of alfalfa revealed new salt and drought stress-related factors involved in seed germination
Salinity and drought are two major environmental factors that limit the growth and yield of many forage crops in semi-arid and arid regions. Alfalfa (Medicago sativa L.) is one of the most important forage crops ...
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Article
A Novel miRNA Sponge Form Efficiently Inhibits the Activity of miR393 and Enhances the Salt Tolerance and ABA Insensitivity in Arabidopsis thaliana
MicroRNAs (miRNAs) regulate gene expression at the post-transcriptional level in eukaryotes by directing target mRNAs for degradation or by repressing translation. MicroRNAs are involved in many processes, inc...
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Article
Open AccessQuantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species
Salt stress is one of the primary abiotic stresses responsible for decreasing crop yields worldwide. Germinating seeds can be greatly influenced by saline conditions. In this study, the physiological and pheno...
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Article
Open AccessHigh-density linkage map construction and map** QTL for yield and yield components in autotetraploid alfalfa using RAD-seq
Alfalfa (Medicago sativa L.) is an important forage crop grown worldwide. Alfalfa is called the “queen of forage crops” due to its high forage yield and nutritional characteristics. The aim of this study was to u...
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Article
Proteomic Analysis of Shoot Tips from Two Alfalfa Cultivars with Different Florescence
Flowering is an indispensable biological process for the complete life cycle of angiosperms, crucial to the regeneration of plants and the continuation of species. In this work, a proteomic approach was applie...
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Article
Open AccessGenetic map** of leaf-related traits in autotetraploid alfalfa (Medicago sativa L.)
Understanding the genetic architecture of leaf-related traits is important for improving alfalfa yield. Leaf size has a great influence on the protein content and yield for alfalfa. In this study, a low-yieldi...
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Article
Open AccessiTRAQ-based comparative proteomic analysis of differences in the protein profiles of stems and leaves from two alfalfa genotypes
To explore the molecular regulatory mechanisms of early stem and leaf development, proteomic analysis was performed on leaves and stems of F genotype alfalfa, with thin stems and small leaves, and M genotype a...
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Article
Identification and characterization of stress responsive homeodomain leucine zipper transcription factors in Medicago truncatula
Homeodomain leucine zipper (HD-ZIP) transcription factors play roles in regulating plant development and responses to abiotic stresses; however, how HD-ZIP genes in Medicago truncatula are involved in abiotic str...
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Article
Open AccessGenome-wide identification, phylogeny and expression analysis of the SPL gene family and its important role in salt stress in Medicago sativa L.
SQUAMOSA promoter-binding protein-like (SPL) transcription factors are widely present in plants and are involved in signal transduction, the stress response and development. The SPL gene family has been character...
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Article
Open AccessOverexpression of the elongation factor MtEF1A1 promotes salt stress tolerance in Arabidopsis thaliana and Medicago truncatula
Elongation factor 1 A (EF1A), an essential regulator for protein synthesis, has been reported to participate in abiotic stress responses and environmental adaption in plants. However, the role of EF1A in abiotic ...
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Article
Genome-wide identification and characterization of filamentation temperature-sensitive H (FtsH) genes and expression analysis in response to multiple stresses in Medicago truncatula
Filamentation temperature-sensitive H (FtsH) is an AAA+ ATP-dependent protease that plays a vital role in plant environmental adaption and tolerance. However, little is known about the function of the FtsH gene f...
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Article
Open AccessA genome-wide study of the lipoxygenase gene families in Medicago truncatula and Medicago sativa reveals that MtLOX24 participates in the methyl jasmonate response
Lipoxygenase (LOX) is a multifunctional enzyme that is primarily related to plant organ growth and development, biotic and abiotic stress responses, and production of flavor-associated metabolites. In higher p...
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Article
Open AccessA genome-wide association study reveals novel loci and candidate genes associated with plant height variation in Medicago sativa
Plant height (PH) is an important agronomic trait influenced by a complex genetic network. However, the genetic basis for the variation in PH in Medicago sativa remains largely unknown. In this study, a comprehen...
