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Article
Open AccessA genome-wide association study reveals novel loci and candidate genes associated with plant height variation in Medicago sativa
Plant height (PH) is an important agronomic trait influenced by a complex genetic network. However, the genetic basis for the variation in PH in Medicago sativa remains largely unknown. In this study, a comprehen...
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Article
Open AccessA genome-wide study of the lipoxygenase gene families in Medicago truncatula and Medicago sativa reveals that MtLOX24 participates in the methyl jasmonate response
Lipoxygenase (LOX) is a multifunctional enzyme that is primarily related to plant organ growth and development, biotic and abiotic stress responses, and production of flavor-associated metabolites. In higher p...
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Article
Genome-wide identification and characterization of filamentation temperature-sensitive H (FtsH) genes and expression analysis in response to multiple stresses in Medicago truncatula
Filamentation temperature-sensitive H (FtsH) is an AAA+ ATP-dependent protease that plays a vital role in plant environmental adaption and tolerance. However, little is known about the function of the FtsH gene f...
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Article
Open AccessOverexpression of the elongation factor MtEF1A1 promotes salt stress tolerance in Arabidopsis thaliana and Medicago truncatula
Elongation factor 1 A (EF1A), an essential regulator for protein synthesis, has been reported to participate in abiotic stress responses and environmental adaption in plants. However, the role of EF1A in abiotic ...
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Article
Open AccessCombining QTL map** and RNA-Seq Unravels candidate genes for Alfalfa (Medicago sativa L.) leaf development
Leaf size affects crop canopy morphology and photosynthetic efficiency, which can influence forage yield and quality. It is of great significance to mine the key genes controlling leaf development for breeding...
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Article
Open AccessMetabolomic changes in crown of alfalfa (Medicago sativa L.) during de-acclimation
Alfalfa is a high-quality forage legume species that is widely cultivated at high latitudes worldwide. However, a decrease in cold tolerance in early spring seriously affects regrowth and persistence of alfalf...
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Article
Open AccessGenome-wide identification, phylogeny and expression analysis of the SPL gene family and its important role in salt stress in Medicago sativa L.
SQUAMOSA promoter-binding protein-like (SPL) transcription factors are widely present in plants and are involved in signal transduction, the stress response and development. The SPL gene family has been character...
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Article
Identification and characterization of stress responsive homeodomain leucine zipper transcription factors in Medicago truncatula
Homeodomain leucine zipper (HD-ZIP) transcription factors play roles in regulating plant development and responses to abiotic stresses; however, how HD-ZIP genes in Medicago truncatula are involved in abiotic str...
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Article
Open AccessDe novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.)
The legume plant alfalfa (Medicago sativa L.) is a widely cultivated perennial forage due to its high protein content, palatability, and strong adaptability to diverse agro-ecological zones. Alfalfa is a self-inc...
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Article
Open AccessiTRAQ-based comparative proteomic analysis of differences in the protein profiles of stems and leaves from two alfalfa genotypes
To explore the molecular regulatory mechanisms of early stem and leaf development, proteomic analysis was performed on leaves and stems of F genotype alfalfa, with thin stems and small leaves, and M genotype a...
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Article
Genome-wide AP2/ERF gene family analysis reveals the classification, structure, expression profiles and potential function in orchardgrass (Dactylis glomerata)
The AP2/ERF transcription factor (TF) family is of great importance in developmental regulation and responses to stress and pathogenic stimuli. Orchardgrass (Dactylis glomerata), a perennial cold-season forage of...
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Article
Open AccessGenetic map** of leaf-related traits in autotetraploid alfalfa (Medicago sativa L.)
Understanding the genetic architecture of leaf-related traits is important for improving alfalfa yield. Leaf size has a great influence on the protein content and yield for alfalfa. In this study, a low-yieldi...
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Article
Proteomic Analysis of Shoot Tips from Two Alfalfa Cultivars with Different Florescence
Flowering is an indispensable biological process for the complete life cycle of angiosperms, crucial to the regeneration of plants and the continuation of species. In this work, a proteomic approach was applie...
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Article
Open AccessHigh-density linkage map construction and map** QTL for yield and yield components in autotetraploid alfalfa using RAD-seq
Alfalfa (Medicago sativa L.) is an important forage crop grown worldwide. Alfalfa is called the “queen of forage crops” due to its high forage yield and nutritional characteristics. The aim of this study was to u...
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Article
Open AccessFull-length transcript sequencing and comparative transcriptomic analysis to evaluate the contribution of osmotic and ionic stress components towards salinity tolerance in the roots of cultivated alfalfa (Medicago sativa L.)
Alfalfa is the most extensively cultivated forage legume. Salinity is a major environmental factor that impacts on alfalfa’s productivity. However, little is known about the molecular mechanisms underlying alf...
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Article
Open AccessQuantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species
Salt stress is one of the primary abiotic stresses responsible for decreasing crop yields worldwide. Germinating seeds can be greatly influenced by saline conditions. In this study, the physiological and pheno...
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Article
Open AccessArabidopsis NUCLEOSTEMIN-LIKE 1 (NSN1) regulates cell cycling potentially by cooperating with nucleosome assembly protein AtNAP1;1
In mammals, nucleostemin (NS), a nucleolar GTPase, is involved in stem cell proliferation, embryogenesis and ribosome biogenesis. Arabidopsis NUCLEOSTEMIN-LIKE 1 (NSN1) has previously been shown to be essential f...
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Article
Open AccessProteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots
Alfalfa (Medicago sativa) is the most extensively cultivated forage legume in the world, and salinity stress is the most problematic environmental factors limiting alfalfa production. To evaluate alfalfa tissue v...
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Article
A Novel miRNA Sponge Form Efficiently Inhibits the Activity of miR393 and Enhances the Salt Tolerance and ABA Insensitivity in Arabidopsis thaliana
MicroRNAs (miRNAs) regulate gene expression at the post-transcriptional level in eukaryotes by directing target mRNAs for degradation or by repressing translation. MicroRNAs are involved in many processes, inc...
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Article
Comparative proteomic analysis of alfalfa revealed new salt and drought stress-related factors involved in seed germination
Salinity and drought are two major environmental factors that limit the growth and yield of many forage crops in semi-arid and arid regions. Alfalfa (Medicago sativa L.) is one of the most important forage crops ...
