-
Article
Open AccessComplete mitochondrial genome of Agrostis stolonifera: insights into structure, Codon usage, repeats, and RNA editing
Plants possess mitochondrial genomes that are large and complex compared to animals. Despite their size, plant mitochondrial genomes do not contain significantly more genes than their animal counterparts. Stud...
-
Article
Genome-wide identification, expression analysis, and transcriptome analysis of the IAA gene family in Zoysia japonica
AUX/IAA is an essential signaling molecule and has great physiological importance in various plants, but its function in Zoysia japonica remains unknown.
-
Article
Open AccessUncovering early transcriptional regulation during adventitious root formation in Medicago sativa
Alfalfa (Medicago sativa L.) as an important legume plant can quickly produce adventitious roots (ARs) to form new plants by cutting. But the regulatory mechanism of AR formation in alfalfa remains unclear.
-
Article
Open AccessMelatonin influences the early growth stage in Zoysia japonica Steud. by regulating plant oxidation and genes of hormones
Zoysia japonica is a commonly used turfgrass species around the world. Seed germination is a crucial stage in the plant life cycle and is particularly important for turf establishment and management. Experiments ...
-
Article
Transcriptome Analysis of the Cytokinin Response in Medicago truncatula
As an important legume plant, Medicago truncatula is a preeminent model for the study of the processes of nitrogen fixation, symbiosis, and legume genomics. The regulatory mechanism of the cytokinin response has ...
-
Article
PacBio single-molecule long-read sequencing shed new light on the transcripts and splice isoforms of the perennial ryegrass
Perennial ryegrass (Lolium perenne), one of the most widely used forage and cool-season turfgrass worldwide, has a breeding history of more than 100 years. However, the current draft genome annotation and transcr...
-
Article
Analysis of transcripts and splice isoforms in Medicago sativa L. by single-molecule long-read sequencing
The full-length transcriptome of alfalfa was analyzed with PacBio single-molecule long-read sequencing technology. The transcriptome data provided full-length sequences and gene isoforms of transcripts in alfa...
-
Article
Open AccessAnalysis of transcripts and splice isoforms in red clover (Trifolium pratense L.) by single-molecule long-read sequencing
Red clover (Trifolium pratense L.) is an important cool-season legume plant, which is the most widely planted forage legume after alfalfa. Although a draft genome sequence was published already, the sequences and...
-
Article
Transcriptome analysis of leaf senescence in red clover (Trifolium pratense L.)
Red clover (Trifolium pratense L.) is an important cool-season legume plant, which is used as forage. Leaf senescence is a critical developmental process that negatively affects plant quality and yield. The regul...
-
Article
Heterologous expression of a novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene, ZjGRP, caused salt sensitivity in Arabidopsis
A novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene was cloned in this study and its overexpression caused salt sensitivity in transge...
-
Article
Molecular cloning and characterization of a gene regulating flowering time from Alfalfa (Medicago sativa L.)
Genes that regulate flowering time play crucial roles in plant development and biomass formation. Based on the cDNA sequence of Medicago truncatula (accession no. AY690425), the LFY gene of alfalfa was cloned. Se...
-
Article
Expression of the alfalfa FRIGIDA-Like Gene, MsFRI-L delays flowering time in transgenic Arabidopsis thaliana
In Arabidopsis, allelic variation at the FRIGIDA (FRI) locus is a major determinant of natural variation in flowering time. We have identified a FRIGIDA like protein gene, MsFRI-L, with unknown function from Medi...
-
Article
Open AccessIsolation and functional characterization of a Medicago sativa L. gene, MsLEA3-1
A full-length cDNA of 1,728 nt, called MsLEA3-1, was cloned from alfalfa by rapid amplification of cDNA ends from an expressed sequence tag homologous to soybean pGmPM10 (accession No. AAA91965.1). MsLEA3-1, enco...
-
Article
Screening of genes induced by salt stress from Alfalfa
An alfalfa cDNA library induced by salt stress was constructed by suppression subtraction hybridization (SSH) technology. Total RNA from 10-day-old seedlings was used as a “driver,” and total RNA from seedling...
-
Article
Molecular cloning and characterization of a novel gene encoding zinc finger protein from Medicago sativa L.
A suppression subtraction hybridization (SSH) cDNA library had been constructed to identify differentially expressed genes. Based on the sequence of an expressed sequence tag (EST) homologous to Pisum sativum zin...
