17 Result(s)
within
Roman Jerala
Science, Humanities and Social Sciences, multidisciplinary
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Article
Open AccessProgrammable de novo designed coiled coil-mediated phase separation in mammalian cells
Membraneless liquid compartments based on phase-separating biopolymers have been observed in diverse cell types and attributed to weak multivalent interactions predominantly based on intrinsically disordered d...
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Article
Open AccessEngineering a scalable and orthogonal platform for synthetic communication in mammalian cells
The rational design and implementation of synthetic mammalian communication systems can unravel fundamental design principles of cell communication circuits and offer a framework for engineering of designer ce...
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Article
Open AccessA multiplexed bacterial two-hybrid for rapid characterization of protein–protein interactions and iterative protein design
Protein-protein interactions (PPIs) are crucial for biological functions and have applications ranging from drug design to synthetic cell circuits. Coiled-coils have been used as a model to study the sequence ...
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Article
Open AccessSegmentation strategy of de novo designed four-helical bundles expands protein oligomerization modalities for cell regulation
Protein–protein interactions govern most biological processes. New protein assemblies can be introduced through the fusion of selected proteins with di/oligomerization domains, which interact specifically with...
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Article
Open AccessCoiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing
The CRISPR/Cas system has emerged as a powerful and versatile genome engineering tool, revolutionizing biological and biomedical sciences, where an improvement of efficiency could have a strong impact. Here we...
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Open AccessRegulation of protein secretion through chemical regulation of endoplasmic reticulum retention signal cleavage
Secreted proteins, such as hormones or cytokines, are key mediators in multicellular organisms. Response of protein secretion based on transcriptional control is rather slow, as it requires transcription, tran...
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Article
Open AccessCoiled-coil heterodimers with increased stability for cellular regulation and sensing SARS-CoV-2 spike protein-mediated cell fusion
Coiled-coil (CC) dimer-forming peptides are attractive designable modules for mediating protein association. Highly stable CCs are desired for biological activity regulation and assay. Here, we report the desi...
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Article
Open AccessAuthor Correction: Self-assembly and regulation of protein cages from pre-organised coiled-coil modules
A Correction to this paper has been published: https://doi.org/10.1038/s41467-021-21969-9
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Article
Open AccessSelf-assembly and regulation of protein cages from pre-organised coiled-coil modules
Coiled-coil protein origami (CCPO) is a modular strategy for the de novo design of polypeptide nanostructures. CCPO folds are defined by the sequential order of concatenated orthogonal coiled-coil (CC) dimer-f...
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Article
Open AccessDesigned folding pathway of modular coiled-coil-based proteins
Natural proteins are characterised by a complex folding pathway defined uniquely for each fold. Designed coiled-coil protein origami (CCPO) cages are distinct from natural compact proteins, since their fold is...
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Article
Open AccessNovel carboxylate-based glycolipids: TLR4 antagonism, MD-2 binding and self-assembly properties
New monosaccharide-based lipid A analogues were rationally designed through MD-2 docking studies. A panel of compounds with two carboxylate groups as phosphates bioisosteres, was synthesized with the same gluc...
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Article
Open AccessNLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway
NLRP3 is a cytosolic sensor triggered by different pathogen- and self-derived signals that plays a central role in a variety of pathological conditions, including sterile inflammation. The leucine-rich repeat ...
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Article
Open AccessPublisher Correction: Phosphodiester backbone of the CpG motif within immunostimulatory oligodeoxynucleotides augments activation of Toll-like receptor 9
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
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Article
Open AccessPublisher Correction: Phosphodiester backbone of the CpG motif within immunostimulatory oligodeoxynucleotides augments activation of Toll-like receptor 9
A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper.
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Article
Open AccessPhosphodiester backbone of the CpG motif within immunostimulatory oligodeoxynucleotides augments activation of Toll-like receptor 9
Toll-like receptor 9 (TLR9) stimulatory CpG-containing oligodeoxynucleotides (ODNs) with phosphorothioate backbones have successfully replaced the naturally occurring agonists of TLR9 in drug development due t...
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Article
Open AccessShort single-stranded DNA degradation products augment the activation of Toll-like receptor 9
Toll-like receptors encounter a diversity of degradation products in endosomes. TLR7 and TLR8 have been shown to be activated by RNA degradation products. Here we show that although TLR9 requires single-strand...
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Article
Open AccessDesign principles for rapid folding of knotted DNA nanostructures
Knots are some of the most remarkable topological features in nature. Self-assembly of knotted polymers without breaking or forming covalent bonds is challenging, as the chain needs to be threaded through prev...
