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Article
Open AccessMonovalent metal ion binding promotes the first transesterification reaction in the spliceosome
Cleavage and formation of phosphodiester bonds in nucleic acids is accomplished by large cellular machineries composed of both protein and RNA. Long thought to rely on a two-metal-ion mechanism for catalysis, ...
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Article
Open AccessCoiled-coil heterodimers with increased stability for cellular regulation and sensing SARS-CoV-2 spike protein-mediated cell fusion
Coiled-coil (CC) dimer-forming peptides are attractive designable modules for mediating protein association. Highly stable CCs are desired for biological activity regulation and assay. Here, we report the desi...
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Article
Open AccessAuthor Correction: Self-assembly and regulation of protein cages from pre-organised coiled-coil modules
A Correction to this paper has been published: https://doi.org/10.1038/s41467-021-21969-9
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Article
Open AccessSelf-assembly and regulation of protein cages from pre-organised coiled-coil modules
Coiled-coil protein origami (CCPO) is a modular strategy for the de novo design of polypeptide nanostructures. CCPO folds are defined by the sequential order of concatenated orthogonal coiled-coil (CC) dimer-f...
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Article
Open AccessDesigned folding pathway of modular coiled-coil-based proteins
Natural proteins are characterised by a complex folding pathway defined uniquely for each fold. Designed coiled-coil protein origami (CCPO) cages are distinct from natural compact proteins, since their fold is...
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Article
A tunable orthogonal coiled-coil interaction toolbox for engineering mammalian cells
Protein interactions guide most cellular processes. Orthogonal hetero-specific protein–protein interaction domains may facilitate better control of engineered biological systems. Here, we report a tunable de n...
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Article
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo
Protein origami using coiled-coil building blocks produces self-assembling polyhedral cages for diverse applications.
