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Article
Open AccessDesigned allosteric protein logic
The regulation of protein function by external or internal signals is one of the key features of living organisms. The ability to directly control the function of a selected protein would represent a valuable ...
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Protocol
Coiled-Coil Interaction Toolbox for Engineering Mammalian Cells
Protein interactions play a crucial role in a variety of biological processes. Therefore, regulation of these interactions has received considerable attention in terms of synthetic biology tool development. Of...
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Article
Open AccessProgrammable de novo designed coiled coil-mediated phase separation in mammalian cells
Membraneless liquid compartments based on phase-separating biopolymers have been observed in diverse cell types and attributed to weak multivalent interactions predominantly based on intrinsically disordered d...
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Article
Open AccessEngineering a scalable and orthogonal platform for synthetic communication in mammalian cells
The rational design and implementation of synthetic mammalian communication systems can unravel fundamental design principles of cell communication circuits and offer a framework for engineering of designer ce...
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Article
Open AccessA multiplexed bacterial two-hybrid for rapid characterization of protein–protein interactions and iterative protein design
Protein-protein interactions (PPIs) are crucial for biological functions and have applications ranging from drug design to synthetic cell circuits. Coiled-coils have been used as a model to study the sequence ...
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Article
Open AccessSegmentation strategy of de novo designed four-helical bundles expands protein oligomerization modalities for cell regulation
Protein–protein interactions govern most biological processes. New protein assemblies can be introduced through the fusion of selected proteins with di/oligomerization domains, which interact specifically with...
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Protocol
Concatenated Coiled-Coil Tag for Highly Efficient, Small Molecule-Inducible Upregulation of Endogenous Mammalian Genes
Regulation of epigenomic functions requires controlled site-specific alteration of epigenetic information. This can be achieved by using designed DNA-binding domains, associated with effector domains, that fun...
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Article
Chemically inducible split protein regulators for mammalian cells
Chemically inducible systems represent valuable synthetic biology tools that enable the external control of biological processes. However, their translation to therapeutic applications has been limited because...
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Protocol
Coiled-Coil Protein Origami: Design, Isolation, and Characterization
Coiled-coil protein origami (CCPO) is a rationally designed de novo protein fold, constructed by concatenating coiled-coil forming segments into a polypeptide chain, that folds into polyhedral nano-cages. To d...
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Article
Open AccessCoiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing
The CRISPR/Cas system has emerged as a powerful and versatile genome engineering tool, revolutionizing biological and biomedical sciences, where an improvement of efficiency could have a strong impact. Here we...
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Article
Open AccessRegulation of protein secretion through chemical regulation of endoplasmic reticulum retention signal cleavage
Secreted proteins, such as hormones or cytokines, are key mediators in multicellular organisms. Response of protein secretion based on transcriptional control is rather slow, as it requires transcription, tran...
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Article
Open AccessCoiled-coil heterodimers with increased stability for cellular regulation and sensing SARS-CoV-2 spike protein-mediated cell fusion
Coiled-coil (CC) dimer-forming peptides are attractive designable modules for mediating protein association. Highly stable CCs are desired for biological activity regulation and assay. Here, we report the desi...
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Article
Open AccessAuthor Correction: Self-assembly and regulation of protein cages from pre-organised coiled-coil modules
A Correction to this paper has been published: https://doi.org/10.1038/s41467-021-21969-9
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Article
Open AccessSelf-assembly and regulation of protein cages from pre-organised coiled-coil modules
Coiled-coil protein origami (CCPO) is a modular strategy for the de novo design of polypeptide nanostructures. CCPO folds are defined by the sequential order of concatenated orthogonal coiled-coil (CC) dimer-f...
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Article
Open AccessDesigned folding pathway of modular coiled-coil-based proteins
Natural proteins are characterised by a complex folding pathway defined uniquely for each fold. Designed coiled-coil protein origami (CCPO) cages are distinct from natural compact proteins, since their fold is...
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Article
Building an international consortium for tracking coronavirus health status
We call upon the research community to standardize efforts to use daily self-reported data about COVID-19 symptoms in the response to the pandemic and to form a collaborative consortium to maximize global gain...
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Article
Publisher Correction: Building an international consortium for tracking coronavirus health status
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
Open AccessMetabolic enzyme clustering by coiled coils improves the biosynthesis of resveratrol and mevalonate
The clustering of biosynthetic enzymes is used in nature to channel reaction products and increase the yield of compounds produced by multiple reaction steps. The coupling of multiple enzymes has been shown to...
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Article
A tunable orthogonal coiled-coil interaction toolbox for engineering mammalian cells
Protein interactions guide most cellular processes. Orthogonal hetero-specific protein–protein interaction domains may facilitate better control of engineered biological systems. Here, we report a tunable de n...
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Article
Open AccessCRISPRa-mediated FOXP3 gene upregulation in mammalian cells
Forkhead box P3+ (FOXP3+) regulatory T cells (Tregs) are a subset of lymphocytes, critical for the maintenance of immune homeostasis. Loss-of-function mutations of the FOXP3 gene in animal models and humans resul...
