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Article
Open AccessDesigned allosteric protein logic
The regulation of protein function by external or internal signals is one of the key features of living organisms. The ability to directly control the function of a selected protein would represent a valuable ...
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Article
Open AccessCoiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing
The CRISPR/Cas system has emerged as a powerful and versatile genome engineering tool, revolutionizing biological and biomedical sciences, where an improvement of efficiency could have a strong impact. Here we...
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Article
Open AccessRegulation of protein secretion through chemical regulation of endoplasmic reticulum retention signal cleavage
Secreted proteins, such as hormones or cytokines, are key mediators in multicellular organisms. Response of protein secretion based on transcriptional control is rather slow, as it requires transcription, tran...
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Article
Open AccessCoiled-coil heterodimers with increased stability for cellular regulation and sensing SARS-CoV-2 spike protein-mediated cell fusion
Coiled-coil (CC) dimer-forming peptides are attractive designable modules for mediating protein association. Highly stable CCs are desired for biological activity regulation and assay. Here, we report the desi...
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Article
Open AccessMetabolic enzyme clustering by coiled coils improves the biosynthesis of resveratrol and mevalonate
The clustering of biosynthetic enzymes is used in nature to channel reaction products and increase the yield of compounds produced by multiple reaction steps. The coupling of multiple enzymes has been shown to...
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Article
Design of fast proteolysis-based signaling and logic circuits in mammalian cells
Cellular signal transduction is predominantly based on protein interactions and their post-translational modifications, which enable a fast response to input signals. Owing to difficulties in designing new uni...
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Article
Open AccessEpCAM homo-oligomerization is not the basis for its role in cell-cell adhesion
Cell-surface tumor marker EpCAM plays a key role in proliferation, differentiation and adhesion processes in stem and epithelial cells. It is established as a cell-cell adhesion molecule, forming intercellular...
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Article
Open AccessPublisher Correction: Phosphodiester backbone of the CpG motif within immunostimulatory oligodeoxynucleotides augments activation of Toll-like receptor 9
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
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Article
Open AccessPublisher Correction: Phosphodiester backbone of the CpG motif within immunostimulatory oligodeoxynucleotides augments activation of Toll-like receptor 9
A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper.
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Article
Open AccessPhosphodiester backbone of the CpG motif within immunostimulatory oligodeoxynucleotides augments activation of Toll-like receptor 9
Toll-like receptor 9 (TLR9) stimulatory CpG-containing oligodeoxynucleotides (ODNs) with phosphorothioate backbones have successfully replaced the naturally occurring agonists of TLR9 in drug development due t...
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Article
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo
Protein origami using coiled-coil building blocks produces self-assembling polyhedral cages for diverse applications.
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Article
Open AccessShort single-stranded DNA degradation products augment the activation of Toll-like receptor 9
Toll-like receptors encounter a diversity of degradation products in endosomes. TLR7 and TLR8 have been shown to be activated by RNA degradation products. Here we show that although TLR9 requires single-strand...
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Erratum
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Gene Expression in Filamentous Fungi: Advantages and Disadvantages Compared to Other Systems
Due to their capacity for producing large amounts of secreted proteins, filamentous fungi have a long history of industrial use for the production of endogenous enzymes. While the genetic engineering tools dev...
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Article
A bistable genetic switch based on designable DNA-binding domains
Bistable switches are fundamental regulatory elements of complex systems, ranging from electronics to living cells. Designed genetic toggle switches have been constructed from pairs of natural transcriptional ...
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Article
Designable DNA-binding domains enable construction of logic circuits in mammalian cells
Synthetic biology requires orthogonal inputs and outputs to avoid undesired crosstalk between genetic constructs. Transcription activator–like effectors (TALEs), which bind diverse DNA sequences and can thus b...
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Article
NLRP3 inflammasome activation in macrophage cell lines by prion protein fibrils as the source of IL-1β and neuronal toxicity
Prion diseases are fatal transmissible neurodegenerative diseases, characterized by aggregation of the pathological form of prion protein, spongiform degeneration, and neuronal loss, and activation of astrocyt...
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Article
Enhancing itaconic acid production by Aspergillus terreus
Aspergillus terreus is successfully used for industrial production of itaconic acid. The acid is formed from cis-aconitate, an intermediate of the tricarboxylic (TCA) cycle, by catalytic action of cis-aconitate d...
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Article
A second binding site for double-stranded RNA in TLR3 and consequences for interferon activation
Toll-like receptor 3 (TLR3) recognizes double-stranded RNA (dsRNA) molecules produced by many viruses and activates an inflammatory response. Synthetic dsRNAs such as small interfering RNAs have been shown to ...
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Protocol
Monolithic Bioreactors for Macromolecules
Enzymes immobilized on solid-phase matrices have found various applications in biotechnology, molecular biology and molecular diagnostics and can serve as industrial catalysts and as specific reagents for anal...
