30 Result(s)
within
Riki Kawaguchi
English
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Protocol
Techniques to Study Specific Cell-Surface Receptor-Mediated Cellular Vitamin A Uptake
STRA6 is a multitransmembrane domain protein that was recently identified as the cell-surface receptor for plasma retinol-binding protein (RBP), the vitamin A carrier protein in the blood. STRA6 binds to RBP w...
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Article
STRA6-Catalyzed Vitamin A Influx, Efflux, and Exchange
Vitamin A has diverse biological functions and is essential for human survival. STRA6 is the high-affinity membrane receptor for plasma retinol binding protein (RBP), the principle and specific carrier of vita...
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Article
Differential and Isomer-Specific Modulation of Vitamin A Transport and the Catalytic Activities of the RBP Receptor by Retinoids
Retinoids are vitamin A derivatives with diverse biological functions. Both natural and artificial retinoids have been used as therapeutic reagents to treat human diseases, but not all retinoid actions are und...
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Article
GDF10 is a signal for axonal sprouting and functional recovery after stroke
Stroke is the leading cause of adult disability. This study shows that the secreted factor GDF10 is a signal for the formation of new brain connections that lead to recovery after stroke and can be manipulated...
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Astrocyte scar formation aids central nervous system axon regeneration
Transected axons fail to regrow in the mature central nervous system. Astrocytic scars are widely regarded as causal in this failure. Here, using three genetically targeted loss-of-function manipulations in ad...
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Article
Astrocytes Can Adopt Endothelial Cell Fates in a p53-Dependent Manner
Astrocytes respond to a variety of CNS injuries by cellular enlargement, process outgrowth, and upregulation of extracellular matrix proteins that function to prevent expansion of the injured region. This astr...
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Article
Open AccessActivity-Dependent Regulation of Alternative Cleavage and Polyadenylation During Hippocampal Long-Term Potentiation
Long-lasting forms of synaptic plasticity that underlie learning and memory require new transcription and translation for their persistence. The remarkable polarity and compartmentalization of neurons raises q...
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Article
Required growth facilitators propel axon regeneration across complete spinal cord injury
Transected axons fail to regrow across anatomically complete spinal cord injuries (SCI) in adults. Diverse molecules can partially facilitate or attenuate axon growth during development or after injury1–3, but ef...
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Article
Open AccessLongitudinal RNA-Seq analysis of acute and chronic neurogenic skeletal muscle atrophy
Skeletal muscle is a highly adaptable tissue capable of changes in size, contractility, and metabolism according to functional demands. Atrophy is a decline in mass and strength caused by pathologic loss of my...
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Astrocyte layers in the mammalian cerebral cortex revealed by a single-cell in situ transcriptomic map
Although the cerebral cortex is organized into six excitatory neuronal layers, it is unclear whether glial cells show distinct layering. In the present study, we developed a high-content pipeline, the large-ar...
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Injured adult neurons regress to an embryonic transcriptional growth state
Grafts of spinal-cord-derived neural progenitor cells (NPCs) enable the robust regeneration of corticospinal axons and restore forelimb function after spinal cord injury1; however, the molecular mechanisms that u...
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Article
Microglia-organized scar-free spinal cord repair in neonatal mice
Spinal cord injury in mammals is thought to trigger scar formation with little regeneration of axons1–4. Here we show that a crush injury to the spinal cord in neonatal mice leads to scar-free healing that permit...
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Open AccessThe effect of Rbfox2 modulation on retinal transcriptome and visual function
Rbfox proteins regulate alternative splicing, mRNA stability and translation. These proteins are involved in neurogenesis and have been associated with various neurological conditions. Here, we analyzed Rbfox2...
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Divergent transcriptional regulation of astrocyte reactivity across disorders
Astrocytes respond to injury and disease in the central nervous system with reactive changes that influence the outcome of the disorder1–4. These changes include differentially expressed genes (DEGs) whose contex...
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Open AccessTranscription factor network analysis identifies REST/NRSF as an intrinsic regulator of CNS regeneration in mice
The inability of neurons to regenerate long axons within the CNS is a major impediment to improving outcome after spinal cord injury, stroke, and other CNS insults. Recent advances have uncovered an intrinsic ...
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Open AccessTranscriptomic architecture of nuclei in the marmoset CNS
To understand the cellular composition and region-specific specialization of white matter — a disease-relevant, glia-rich tissue highly expanded in primates relative to rodents — we profiled transcriptomes of ...
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Open AccessClinically relevant small-molecule promotes nerve repair and visual function recovery
Adult mammalian injured axons regenerate over short-distance in the peripheral nervous system (PNS) while the axons in the central nervous system (CNS) are unable to regrow after injury. Here, we demonstrated ...
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Article
Open AccessP300 promotes tumor recurrence by regulating radiation-induced conversion of glioma stem cells to vascular-like cells
Glioma stem cells (GSC) exhibit plasticity in response to environmental and therapeutic stress leading to tumor recurrence, but the underlying mechanisms remain largely unknown. Here, we employ single-cell and...
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Article
Open AccessElk-1 regulates retinal ganglion cell axon regeneration after injury
Adult central nervous system (CNS) axons fail to regenerate after injury, and master regulators of the regenerative program remain to be identified. We analyzed the transcriptomes of retinal ganglion cells (RG...
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Article
Open AccessBroad transcriptomic dysregulation occurs across the cerebral cortex in ASD
Neuropsychiatric disorders classically lack defining brain pathologies, but recent work has demonstrated dysregulation at the molecular level, characterized by transcriptomic and epigenetic alterations1–3. In aut...
