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Article
Evaluation of a simple protein free medium that supports high levels of monoclonal antibody production
A simple protein free medium was formulated and tested in suspension culture using three hybridoma cell lines. The medium, referred to as CDSS (Chemically Defined Serum Substitutes), consisted of the basal med...
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Chapter
Using Oxygen Uptake Rates to Time Infections in the Baculovirus Expression Vector System
In this article, the problem of optimising insect cell cultures infected with a recombinant Baculovirus is discussed. A model of the infection of insect cells in batch cultures suggests that the maximum achiev...
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Chapter
Enhanced Productivity in Insect Cell Culture by Control of the Chemical Environment
Low cell density, suboptimal nutrient environment and increased down time, and labour of repeated batch fermentations impose major limitations to high product yields in recombinant baculovirus infected insect ...
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Chapter
The Effect of Concentration and Hydraulic Shock Loads on the Performance of a Two-Stage High-Rate Anaerobic Wastewater Treatment System: Prediction and Validation
An improved version of a mechanistic mathematical model of anaerobic degradation processes has been developed. It was incorporated into three types of high-rate reactor configuration; namely, single-stage, two...
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Chapter
Improved Monoclonal Antibody Production via Controlled Feeding Strategies during Fedbatch Cultures of a Hybridoma Cell Line Utilizing Protein Free Media
A quantitative study on the effect of feed concentration and feed rate during a fedbatch cultivation system on the performance of the SPO1 hybridoma cell line using a protein free media is presented. The objec...
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Article
Data analysis of plasmid stability in continuous culture of recombinantSaccharomyces cerevisiae
A simple method using non-linear regression is developed to analyse experimental data from plasmid stability studies of recombinantSaccharomyces cerevisiae grown in continuous cultures with non-selective and sele...
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Article
A packed bed solid-state cultivation system for the production of animal feed: Cultivation, drying and product quality
A standing column packed bed bioreactor was constructed to enable packed bed operation during solid-state cultivation (SSC) of sago starch byR. oligosporus UQM 145F, to be followed byin situ drying of the microbi...
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Article
Avoiding rapid growth at high cell densities: A potentially important optimisation criterion for hybridoma cultures
Inhibition caused by rapid changes in the environment has earlier been observed in hybridoma cultures following deliberate step-changes in the culture environment. This paper presents evidence of similar effec...
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Article
Modelling the growth and protein production by insect cells following infection by a recombinant baculovirus in suspension culture
A mathematical model has been developed to describe the growth and infection of insect cells by recombinant baculoviruses. The model parameters were determined from a series of independent experiments involvin...
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Chapter
Improved Production of Recombinant Proteins by the Baculovirus Expression System
The production of a model recombinant protein (β -galactosidase) by rAcNPV infected Sf-9 (Spodoptera frugiperda) insect cells was enhanced by modification of the medium environment. Improved production by chemica...
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Chapter
Continuous Monoclonal Antibody Production by a Composite Gel Perfusion Bioreactor in a Protein-Free Medium
An alginate/agarose composite gel system for the immobilisation of hybridoma cells, was developed, integrating the simplicity of gelation but instability of calcium alginate and the stability, rigidity and por...
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Article
Cost optimal operation of an industrial two-stage high-rate anaerobic treatment plant
An economic model which incorporates the major costs associated with the operation of an industrial anaerobic reactor was developed. This model was then used to optimise the operation of a two-stage anaerobic ...
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Article
Experience in scale-up of homogeneous perfusion culture for hybridomas
A perfusion system for production of monoclonal antibodies was developed using an externally-mounted, hollow-fibre cartridge. The experimental apparatus was operated for 420 h and demonstrated increased steady...
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Article
Protein enrichment of sago starch by solid-state fermentation with Rhizopus spp.
Protein enrichment of sago starch of three different diameters was investigated both in flask culture and under forced aeration in a packed-bed fermenter using two strains of Rhizopus. Protein production by R. ol...
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Article
Weaning of three hybridoma cell lines to serum free low protein medium
A general weaning procedure is described which allowed a range of hybridomas to be weaned readily off serum without loss of antibody production. Initial work was carried out with one cell line only (SPO1 cells...
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Chapter
Byproducts from Zymomonas mobilis
Z. mobilis is a microorganism that is not only an extremely efficient producer of alcohol, but also is capable of producing other metabolites in high concentrations under the correct culture conditions. The techn...
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Article
Mode of growth ofRhizopus oligosporus on a model substrate in solid-state fermentation
The growth ofRhizopus oligosporus on a model solid substrate consisting of cassava starch and other nutrients embedded in a kappa-carrageenan matrix was followed microscoplcally. There were two distinct growth ph...
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Article
Hybridoma growth limitations: The roles of energy metabolism and ammonia production
Energy metabolism and the production of ammonia in hybridoma cell culture and its inhibitory effects on cell growth are reviewed. The interactive roles of glucose and glutamine metabolism affect the rate of pr...
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Article
Stabilisation of a recombinant yeast plasmid in nonselective medium by cyclic growth rate changes
The 2μm based yeast plasmid, pLG669-z, was stably maintained in aS. cerevisiae strain during continuous culture with cyclic growth rate changes. In the absence of a selective mechanism, the fraction of plasmid co...
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Article
Control of anaerobic digesters using Generic Model Control
This paper describes the improved effluent control of industrial anaerobic digestion treatment systems. Previous research has shown the possibility of applying a model-based adaptive control technique to these...
