Abstract
The growth ofRhizopus oligosporus on a model solid substrate consisting of cassava starch and other nutrients embedded in a kappa-carrageenan matrix was followed microscoplcally. There were two distinct growth phases: (1) germination of spores and development of mycellum to form a loose network over the whole substrate surface; (2) increase in the density of the mycellum. Although some penetrative hyphae were produced, the biomass was malnly restricted to the surface. Consequently, starch utilization was greatest near the surface. Based on these observations a descriptive model for the growth ofRhizopus ollgosporus on the model substrate was proposed. The five steps are: (1) release of glucoamylase by the mycelium; (2) diffusion of the glucoamylase to the starch; (3) hydrolysis of the starch by the glucoamylase, releasing glucose; (4) diffusion of glucose; (5) absorption of glucose by the mycellum at the surface to produce new blomass.
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Aufeuvre, M.A. &Raimbault, M. 1982 Scanning electron microscope study of development ofAspergillus niger van Tieghem on a solid substrate.Comptes Rendus des Seances de l'Academie des Sciences. Series III. Sciences de la Vie 294, 949–956.
Burnett, J.H. 1976Fundamentals of Mycology. London: Edward Arnold.
Carrizales, V. &Jaffe, W. 1986 Solid state fermentation: an appropriate biotechnology for develo** countries.Interciencia 11, 9–15.
Daubresse, P., Ntibashirwa, S., Gheysen, A. &Meyer, J.A. 1987 A process for protein enrichment of cassava by solid substrate fermentation in rural conditions.Biotechnology and Bioengineering 29, 962–968.
Georgiou, G. &Shuler, M.L. 1986 A computer model for the growth and differentiation of a fungal colony on solid substrate.Biotechnology and Bioengineering 28, 405–416.
Jurnus, A.M. &Sundberg, W.J. 1976 Penetration ofRhizopus oligosporus into soybeans in tempeh.Applied and Environmental Microbiology 32, 284–287.
Kronenberg, H.J. 1984 Reduction of incubation time for tempeh fermentation by use of pregerminated inoculum.Economic Botany 38, 433–438.
Laukevics, J.J., Apsite, A.F., Viesturs, U.S. &Tengerdy, R.P. 1985 Steric hindrance of growth of filamentous fungi in solid substrate fermentation of wheat straw.Biotechnology and Bioengineering 27, 1687–1691.
Medwid, R.D. &Grant, D.W. 1984 Germination ofRhizopus oligosporus sporangiospores.Applied and Environmental Microbiology 48, 1067–1071.
Miranda, M., Siso, I.G., Gonzalez, P., Murdo, M.A. &Miron, J. 1987 Amylolysis in systems with α-amylase and glucoamylase. A comparative study of six procedures of evaluation.Biotechnology Techniques 1, 195–200.
Mitchell, D.A., Doelle, H.W. &Greenfield, P.F. 1989 Suppression of penetrative hyphae ofRhizopus oligosporus by membrane filters in a model solid-state fermentation system.Biotechnology Techniques 3, 45–50.
Mitchell, D.A., Greenfield, P.F. &Doelle, H.W. 1986 A model substrate for solid-state fermentation.Biotechnology Letters 8, 827–832.
Mitchell, D.A., Greenfield, P.F. &Doelle, H.W. 1988 Development of a model solid-state fermentation system.Biotechnology Techniques 2, 1–6.
Moo-Young, M., Moreira, A.R. &Tengerdy, R.P. 1983 Principles of solid-substrate fermentation. InThe Filamentous Fungi, Vol. 4, eds. Smith, J.E., Berry, D.R. & Kristiansen, B., pp. 117–144, London: Edward Arnold.
Oliver, S.G. &Trinci, A.P.J. 1985 Modes of growth of bacteria and fungi. InComprebensive Biotechnology, Vol. 1, ed. Moo-Young, M., pp. 159–187. New York: Pergamon Press.
Pirt, S.J. 1967 A kinetic study of the mode of growth of surface colonies of bacteria and fungi.Journal of General Microbiology 47, 181–197.
Ramos-Valdivia, A., De la Torre, M. &Casas-Campillo, C. 1983 Solid state fermentation of cassava withRhizopus oligosporus NRRL 2710. InProduction and Feeding of Single-Cell Protein, eds. Ferranti, M.P. & Fiechter, A., pp. 104–111. London and New York: Applied Science Publishers.
Trevelyan, W.E. 1974 The enrichment of cassava by moist-solids fermentation.Tropical Science 16, 179–194.
Wang, H.L. &Hesseltine, C.W. 1982 Oriental fermented foods. InPrescott and Dunn's Industrial Microbiology, ed. Reed, G., pp. 492–538. Westport, CT: AVI Publishing.
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Mitchell, D.A., Greenfield, P.F. & Doelle, H.W. Mode of growth ofRhizopus oligosporus on a model substrate in solid-state fermentation. World J Microbiol Biotechnol 6, 201–208 (1990). https://doi.org/10.1007/BF01200942
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DOI: https://doi.org/10.1007/BF01200942