17 Result(s)
within
Nina Cabezas-Wallscheid
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Article
A call for accessible tools to unlock single-cell immunometabolism research
Recent technological advances permit the profiling of metabolic changes in single cells, which sheds light on how metabolism regulates immune responses. We advocate for accessible and standardized tools to red...
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Open AccessProstaglandin E2 controls the metabolic adaptation of T cells to the intestinal microenvironment
Immune cells must adapt to different environments during the course of an immune response. Here we study the adaptation of CD8+ T cells to the intestinal microenvironment and how this process shapes the establish...
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Mentoring the next generation of cell biologists
As Nature Cell Biology turns 25 years old, we asked cell biologists across the globe to share their thoughts on what a productive mentor–mentee relationship looks like and their views on training the next generat...
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Open AccessVarID2 quantifies gene expression noise dynamics and unveils functional heterogeneity of ageing hematopoietic stem cells
Variability of gene expression due to stochasticity of transcription or variation of extrinsic signals, termed biological noise, is a potential driving force of cellular differentiation. Utilizing single-cell ...
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Open AccessHyaluronic acid–GPRC5C signalling promotes dormancy in haematopoietic stem cells
Bone marrow haematopoietic stem cells (HSCs) are vital for lifelong maintenance of healthy haematopoiesis. In inbred mice housed in gnotobiotic facilities, the top of the haematopoietic hierarchy is occupied b...
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Open AccessTFEB induces mitochondrial itaconate synthesis to suppress bacterial growth in macrophages
Successful elimination of bacteria in phagocytes occurs in the phago-lysosomal system, but also depends on mitochondrial pathways. Yet, how these two organelle systems communicate is largely unknown. Here we i...
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Open AccessGab2 deficiency prevents Flt3-ITD driven acute myeloid leukemia in vivo
Internal tandem duplications (ITD) of the FMS-like tyrosine kinase 3 (FLT3) predict poor prognosis in acute myeloid leukemia (AML) and often co-exist with inactivating DNMT3A mutations. In vitro studies implicate...
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Open AccessP2Y12-dependent activation of hematopoietic stem and progenitor cells promotes emergency hematopoiesis after myocardial infarction
Emergency hematopoiesis is the driving force of the inflammatory response to myocardial infarction (MI). Increased proliferation of hematopoietic stem and progenitor cells (LSK) after MI enhances cell producti...
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Open AccessPublisher Correction: Chemotherapy-induced transposable elements activate MDA5 to enhance haematopoietic regeneration
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Open AccessChemotherapy-induced transposable elements activate MDA5 to enhance haematopoietic regeneration
Haematopoietic stem cells (HSCs) are normally quiescent, but have evolved mechanisms to respond to stress. Here, we evaluate haematopoietic regeneration induced by chemotherapy. We detect robust chromatin reor...
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Open AccessNiche derived netrin-1 regulates hematopoietic stem cell dormancy via its receptor neogenin-1
Haematopoietic stem cells (HSCs) are characterized by their self-renewal potential associated to dormancy. Here we identify the cell surface receptor neogenin-1 as specifically expressed in dormant HSCs. Loss ...
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Characterizing the In Vivo Role of Candidate Leukemia Stem Cell Genes
Acute myeloid leukemia (AML) is a disease caused by multiple distinct genomic events in the hematopoietic stem cell and progenitor compartment. To gain insight into the link between genetic mutations in AML an...
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Haematopoietic stem cells in perisinusoidal niches are protected from ageing
With ageing, intrinsic haematopoietic stem cell (HSC) activity decreases, resulting in impaired tissue homeostasis, reduced engraftment following transplantation and increased susceptibility to diseases. Howev...
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Open AccessThe long non-coding RNA Meg3 is dispensable for hematopoietic stem cells
The long non-coding RNA (lncRNA) Maternally Expressed Gene 3 (Meg3) is encoded within the imprinted Dlk1-Meg3 gene locus and is only maternally expressed. Meg3 has been shown to play an important role in the regu...
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Protocol
Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantation Assays
Healthy hematopoietic stem cells (HSCs) are capable to self-renew and reconstitute the complete hematopoietic system. Upon aging, there is an increased incidence of blood-related diseases. Age-related phenotyp...
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Single-cell characterization of haematopoietic progenitors and their trajectories in homeostasis and perturbed haematopoiesis
The dynamics of haematopoietic stem cell differentiation and the hierarchy of oligopotent stem cells in the bone marrow remain controversial. Here we dissect haematopoietic progenitor populations at single cel...
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Open AccessTetracycline-controlled transgene activation using the ROSA26-iM2-GFP knock-in mouse strain permits GFP monitoring of DOX-regulated transgene-expression
Conditional gene activation is an efficient strategy for studying gene function in genetically modified animals. Among the presently available gene switches, the tetracycline-regulated system has attracted con...
